scholarly journals Polycyclic Aromatic Hydrocarbons Degradation by Aquatic Bacteria Isolated from Khazar Sea, the World’s Largest Lake

2020 ◽  
Vol 27 (1) ◽  
pp. 121-130
Author(s):  
Vida Ebrahimi ◽  
Shirin Eyvazi ◽  
Soheila Montazersaheb ◽  
Parivar Yazdani ◽  
Mohammad Amin Hejazi ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Carbon Source ◽  
Aromatic Hydrocarbons ◽  
Sea Water ◽  
Environmental Pollutants ◽  
Bacterial Isolates ◽  
Mazandaran Province ◽  
Degrading Bacteria ◽  
Lung Dysfunction ◽  
Polycyclic Aromatic

Background: Aquatic microorganisms have an important role in the bioremediation of environmental pollutants. Polycyclic Aromatic Hydrocarbons (PAHs) are described as dangerous pollutants that can bind covalently to the nucleic acids, causing mutations. Therefore, they have carcinogenic and toxic properties. Also, are involved in diseases such as asthma, lung dysfunction, and chronic bronchitis. This study aimed to isolate and characterize aquatic bio-degrading bacteria from the world’s largest lake, Khazar, with the ability to use PAHs as only carbon source. Methods: Samples were taken from the estuary of Siah Rud River (Mazandaran province, Iran) and Fereydunkenar beach leading to isolation of twenty-three bacteria on marine agar and sea water media. The isolates were cultured on separate ONR7a medium, each supplemented with only one PAH; as the sole carbon source; including naphthalene, phenanthrene, and anthracene. Results: Eleven bacterial isolates were able to grow on supplemented media: TBZ-E1, TBZ-E2, TBZ-E3, TBZ-S12, TBZ-S16, TBZ-E20, TBZ-SF2, TBZ-F1, TBZ-F2, TBZ-F3 and TBZ2. These isolates belong to Alteromonas, Marivivens, Pseudoalteromonas, Vibrio, Shewanella, Photobacterium, Mycobacterium and Pseudomonas genera. The qualitative analysis showed that the consortium of isolates TBZ-F1, TBZ-F2, TBZ-F3, TBZ-SF2, and TBZ2 displayed the highest degradation rate for phenanthrene and naphthalene. Naphthalene, phenanthrene, and anthracene were potently degraded by TBZ2 and TBZ-SF2 and accordingly were subjected to measure degradation potential of mentioned PAHs. Conclusion: The bacterial isolates of Caspian lake have a critical duty in biodegradation of PAHs. These isolates are representative samples of the bacterial population of this lake, participating in the purification process of this habitat.

scholarly journals Initial microbial degradation of polycyclic aromatic hydrocarbons

2016 ◽  
Vol 22 (3) ◽  
pp. 293-299 ◽  
Author(s):  
Jelena Milic ◽  
Jelena Avdalovic ◽  
Tatjana Solevic-Knudsen ◽  
Gordana Gojgic-Cvijovic ◽  
Tanja Jednak ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Environmental Pollutants ◽  
Bacterial Isolates ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Polycyclic Aromatic ◽  
Almost All ◽  
Rhodococcus Sp

The group of polycyclic aromatic hydrocarbons (PAHs) are very hazardous environmental pollutants because of their mutagenic, carcinogenic and toxic effects on living systems. The aim of this study was to examine and compare the ability and efficiency of selected bacterial isolates obtained from oil-contaminated areas to biodegrade PAHs. The potential of the bacteria to biodegrade various aromatic hydrocarbons was assessed using the 2,6-dichlorophenol-indophenol assay. Further biodegradation of PAHs was monitored by gravimetric and gas-chromatographic analysis. Among the eight bacterial isolates, identified on the basis of 16S rDNA sequences, two isolates, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05, had the ability to grow on and utilize almost all examined hydrocarbons. Those isolates were further examined for biodegradation of phenanthrene and pyrene, as single substrates, and as a mixture, in vitro for ten days. After three days, both isolates degraded a significant amount phenanthrene, which has a simpler chemical structure than pyrene. Planomicrobium sp.RNP01 commenced biodegradation of pyrene in the PAH mixture only after it had almost completly degraded phenanthrene. The isolated and characterized bacteria, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05, have shown high bioremediation potential and are likely candidates to be used for degradation of highly toxic PAHs in contaminated areas.

scholarly journals Isolation and characterization of bacteria with multiple traits: Hydrocarbon degradation, antibiotic-resistant and metal tolerant

2019 ◽  
Vol 10 (4) ◽  
pp. 3789-3795 ◽  
Author(s):  
Neeta Bhagat ◽  
Pranita Roy ◽  
Sohini Singh ◽  
Tanu Allen
Keyword(s):  
Heavy Metals ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Soil Pollution ◽  
Aromatic Hydrocarbons ◽  
Well Being ◽  
Antibiotic Resistant ◽  
Morphological Studies ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic

Increasing soil pollution all over the world has instigated global concerns as enormous quantities of toxic chemicals and heavy metals like cadmium, lead, mercury, petrochemicals, insecticides, polycyclic aromatic hydrocarbons (PAHs) and chlorophenols are finding their way into the environment, affecting the land and soil, causing soil pollution and thus posing a threat and menace to health and well- being of people and ecosystem. The ubiquitous dissemination, low bioavailability, high perseverance of contaminants like poly-hydrocarbon and metals in soil have the potentially destructive effects to human health, envisages to study the biodegradation of PAHs (polycyclic aromatic hydrocarbons) and PACs (polycyclic aromatic compounds). The diversity of micro-organisms that diminish the PAHs/PACs can be utilized in the advancement of bioremediation techniques. The role of metal-tolerant, (PAH)-degrading bacteria helps in the biodegradation of organic compounds at miscellaneous polluted sites. The isolation of (PAHs)-degrading bacteria from contaminated soil samples collected from garages and petrol pumps of Delhi and NCR region was carried out in the present study.  Also, the bacterial samples were tested for the tolerance towards 4 heavy metals- arsenic (As), lead (Pb), cadmium (Cd), and mercury (Hg). Morphological studies and biochemical tests were conducted to find the genera of the bacterial samples. The study indicates that hydrocarbons were degraded by the isolates P1, P2, P4, P5, P5*, G1, G3. These isolates were also found to be tolerant at a high concentration of metals (Arsenic, Cadmium, Mercury, and Lead) as minimum inhibitory concentration (MIC) was also calculated. Antibiotic susceptibility of the isolates was tested against various antibiotics. Thus the study suggests that the isolates identified as Pseudomonas aeruginosa, Acinetobacter baumanii, and Klebsiella pneumoniae are not only PAH-degrading but metal-tolerant and antibiotic-resistant too and are of immense potential for bioremediation of contaminated soils.

Impact of Inoculation Protocols, Salinity, and pH on the Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) and Survival of PAH-Degrading Bacteria Introduced into Soil

1998 ◽  
Vol 64 (1) ◽  
pp. 359-362 ◽  
Author(s):  
Matthias Kästner ◽  
Maren Breuer-Jammali ◽  
Bernd Mahro
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Pore Water ◽  
Aromatic Hydrocarbons ◽  
Soil Bacteria ◽  
Water Salinity ◽  
Sphingomonas Paucimobilis ◽  
Pah Degradation ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic ◽  
Pore Water Salinity

ABSTRACT Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of the introduced bacteria were monitored in native and sterilized soil at different pHs. Introduction with mineral medium inhibited PAH degradation by the autochthonous microflora and by the strains tested. After introduction with water (without increase of the pore water salinity), no inhibition of the autochthonous microflora was observed and both strains exhibited PAH degradation.

scholarly journals Assessment of Polycyclic Aromatic Hydrocarbons in Agricultural Soils of Teskelewu Community and Environs Impacted By Gas Flaring in Delta State, Nigeria

2019 ◽  
Vol 23 (10) ◽  
pp. 1861-1866
Author(s):  
A.A. Enuneku ◽  
B. Kubeyinje
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Agricultural Soils ◽  
Environmental Pollutants ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Gas Flaring ◽  
Flame Ionization ◽  
Polycyclic Aromatic ◽  
Living Organisms

Polycyclic aromatic hydrocarbons (PAHs) are emerging ubiquitous environmental pollutants with reported carcinogenic, mutagenic and teratogenic potentials on living organisms and humans. The objective of this study was to assess the concentrations of PAHs in agricultural soils of Tsekelewu Community and environs (Egbema Kingdom) in Warri North Local Government Area of Delta State, Nigeria impacted by gas flaring using gas chromatograph system Agilent 5890 Series 11 gas chromatograph equipped with Flame ionization detector (GC-FID). The results revealed that station 1 and 2 were fairly contaminated; with mean total PAH concentration of 236.40 μg/kg and 279.75 μg/kg respectively. Stations 3, 4, 6, 7 and 9 having mean total PAH concentration of 692.45μg/kg, 726.22 μg/kg, 739.82 μg/kg, 609.38 μg/kg, and 772.99 μg/kg were moderately contaminated. Heavy contamination was recorded in station 5 and station 8 with mean PAH concentrations of 1231.08μg/kg and 1224.33 μg/kg. The dominant PAHs in soil samples were benzo(a)anthracene, phananthrene and chrysene with benzo(a)anthracene having the highest concentration across all sample stations. The PAH concentration observed in this study is thus a serious cause for concern since soil contamination from gas flaring will likely continue. Measures that will attenuate PAHs concentrations in soil should be adopted as farmers are likely exposed by largely inhalation.Keywords: Hydrocarbons; Contamination; Agricultural soils; Gas flaring 

Characteristics of phenanthrene-degrading bacteria isolated from soils contaminated with polycyclic aromatic hydrocarbons

1998 ◽  
Vol 44 (8) ◽  
pp. 743-752 ◽  
Author(s):  
Michael D Aitken ◽  
William T Stringfellow ◽  
Robert D Nagel ◽  
Chikoma Kazunga ◽  
Shu-Hwa Chen
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soils ◽  
Acid Methyl Ester ◽  
Aqueous Solubility ◽  
Bacterial Strains ◽  
Phenanthrene Degradation ◽  
Acid Methyl ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic

Ten bacterial strains were isolated from seven contaminated soils by enrichment with phenanthrene as the sole carbon source. These isolates and another phenanthrene-degrading strain were examined for various characteristics related to phenanthrene degradation and their ability to metabolize 12 other polycyclic aromatic hydrocarbons (PAH), ranging in size from two to five rings, after growth in the presence of phenanthrene. Fatty acid methyl ester analysis indicated that at least five genera (Agrobacterium, Bacillus, Burkholderia, Pseudomonas, and Sphingomonas) and at least three species of Pseudomonas were represented in this collection. All of the strains oxidized phenanthrene according to Michaelis-Menten kinetics, with half-saturation coefficients well below the aqueous solubility of phenanthrene in all cases. All but one of the strains oxidized 1-hydroxy-2-naphthoate following growth on phenanthrene, and all oxidized at least one downstream intermediate from either or both of the known phenanthrene degradation pathways. All of the isolates could metabolize (oxidize, mineralize, or remove from solution) a broad range of PAH, although the exact range and extent of metabolism for a given substrate were unique to the particular isolate. Benz[a]anthracene, chrysene, and benzo[a]pyrene were each mineralized by eight of the strains, while pyrene was not mineralized by any. Pyrene was, however, removed from solution by all of the isolates, and the presence of at least one significant metabolite from pyrene was observed by radiochromatography for the five strains in which such metabolites were sought. Our results support earlier indications that the mineralization of pyrene by bacteria may require unique metabolic capabilities that do not appear to overlap with the determinants for mineralization of phenanthrene or other high molecular weight PAH.Key words: kinetics, polycyclic aromatic hydrocarbons, phenanthrene, mineralization, benzo[a]pyrene.

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