Microbial Diversity and Community Structure of Postdisturbance Forest Soils as Determined by Sole-Carbon-Source Utilization Patterns

1997 ◽  
Vol 34 (2) ◽  
pp. 125-130 ◽  
Author(s):  
W.J. Staddon *, † , L.C. Duchesne ◽  
J.T. Trevors
Keyword(s):  
Community Structure ◽  
Carbon Source ◽  
Microbial Diversity ◽  
Forest Soils ◽  
Sole Carbon Source ◽  
Carbon Source Utilization ◽  
Utilization Patterns ◽  
Sole Carbon Source Utilization

General (Biolog GN) versus site-relevant (pollutant-dependent) sole-carbon-source utilization patterns as a means to approaching community functioning

1998 ◽  
Vol 44 (10) ◽  
pp. 913-919 ◽  
Author(s):  
Petra M Becker ◽  
Ulrich Stottmeister
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Carbon Source Utilization ◽  
Community Functioning ◽  
Biolog System ◽  
Utilization Patterns ◽  
Biolog Gn ◽  
The Individual ◽  
Sole Carbon Source Utilization

Biolog community-level sole-carbon-source utilization patterns are widely applied to distinguish between the microbiota of different habitats or reveal disturbances in microbial ecosystems. Our objective was to examine whether the metabolic diversity measured in the Biolog system could be related to community functioning, thus providing more information than being merely discriminative for communities. To answer this question, we compared the percentages of specific pollutant utilizers with the percentages of the degraders of distinct general (Biolog) substrates in arbitrary samples of isolates, employing in both cases the isolate sample assay (ISA). Samples for arbitrarily selected isolates were taken from in situ enclosures situated in the anaerobic lignite-carbonization effluent Lake Schwelvollert (district of Weißenfels, Saxony-Anhalt, Germany). A different aerobic-carbonization wastewater treatment was carried out in each of the in situ enclosures. It was shown that distinct arbitrarily compiled isolate samples, which displayed similar functional patterns in terms of the degradation of pollutant-related compounds, exhibited significantly different general metabolic capacities as measured using Biolog kits. The similar pollutant-degradation potentials of the arbitrary isolates indicated that the site's substrate supply plays an important role in deciding which bacteria can become indigenous, seemingly leaving room for the otherwise varying properties of the individual community members.Key words: lignite-carbonization wastewater, ISA (isolate sample assay), sole-carbon-source utilization, Biolog, community functioning.

scholarly journals Analysis of the Dynamics of Bacterial Communities in the Rhizosphere of the Chrysanthemum via Denaturing Gradient Gel Electrophoresis and Substrate Utilization Patterns

1998 ◽  
Vol 64 (12) ◽  
pp. 4950-4957 ◽  
Author(s):  
Bernadette M. Duineveld ◽  
Alexandre S. Rosado ◽  
Jan Dirk van Elsas ◽  
Johannes A. van Veen
Keyword(s):  
Carbon Source ◽  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
Metabolic Profile ◽  
Sole Carbon Source ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Root Tip ◽  
Carbon Source Utilization ◽  
Gradient Gel Electrophoresis ◽  
Sole Carbon Source Utilization

ABSTRACT In order to gain a better understanding of the spatial and temporal dynamics of bacterial communities of the rhizosphere of the chrysanthemum, two complementary methods were used: a molecular bacterial community profiling method, i.e., 16S rRNA gene-based PCR followed by denaturing gradient gel electrophoresis (DGGE), and an agar plate method in which 11 sole-carbon-source utilization tests were used. The DGGE patterns showed that the bacterial communities as determined from direct rhizosphere DNA extracts were largely stable along developing roots of the chrysanthemum, with very little change over time or between root parts of different ages. The patterns were also similar to those produced with DNA extracts obtained from bulk soil samples. The DGGE patterns obtained by using microbial colonies from dilution plates as the source of target DNA were different from those found with the direct DNA extracts. Moreover, these patterns showed differences among plant replicates but also among replicate plates. Results obtained with the sole-carbon-source utilization tests indicated that the metabolic profile of the bacterial communities in the rhizosphere of the root tip did not change substantially during plant growth. This suggests selective development of specific bacterial populations by the presence of a root tip. On the other hand, the metabolic profile of bacterial communities in the rhizosphere of the root base changed during plant growth. With eight sole-carbon-source utilization tests, a significant effect of the development stage of the plant on the number of bacteria which were able to grow on these carbon sources was observed.

Analysis of fungal communities by sole carbon source utilization profiles

2001 ◽  
Vol 45 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Jeffrey S Buyer ◽  
Daniel P Roberts ◽  
Patricia Millner ◽  
Estelle Russek-Cohen
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Fungal Communities ◽  
Carbon Source Utilization ◽  
Sole Carbon Source Utilization

Assessment of self-organizing maps to analyze sole-carbon source utilization profiles

2005 ◽  
Vol 62 (1) ◽  
pp. 89-102 ◽  
Author(s):  
Joséphine Leflaive ◽  
Régis Céréghino ◽  
Michaël Danger ◽  
Gérard Lacroix ◽  
Loïc Ten-Hage
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Carbon Source Utilization ◽  
Self Organizing Maps ◽  
Sole Carbon Source Utilization ◽  
Self Organizing
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