A CHEMICALLY DEFINED MEDIUM FOR GROWTH OF PASTEURELLA MULTOCIDA

1966 ◽  
Vol 12 (5) ◽  
pp. 933-937 ◽  
Author(s):  
Laurence P. Watko
Keyword(s):  
Pasteurella Multocida ◽  
Defined Medium ◽  
Good Growth ◽  
Chemically Defined Medium ◽  
Biochemical Characteristics ◽  
Metallic Salts

The chemically defined medium of McKenzie et al. was modified by increasing dextrose and buffer and adding metallic salts. This modified medium supported growth of nine different isolates of P. multocida through 10 serial transfers. One isolate (strain X-73) was successfully subcultured through more than 100 transfers in the modified medium without change of its biochemical characteristics. Viable organisms were recovered from static cultures after 45 days" incubation at 37 C. A 1/10-ml inoculum containing approximately two or three organisms was sufficient to produce good growth within 24–48 h.

scholarly journals The Growth Of Rhizobium in Synthetic Media

1961 ◽  
Vol 14 (3) ◽  
pp. 349 ◽  
Author(s):  
FJ Bergersen
Keyword(s):  
Amino Acids ◽  
Nitrogen Source ◽  
Exponential Growth ◽  
Defined Medium ◽  
Complete Medium ◽  
Good Growth ◽  
Chemically Defined Medium ◽  
Sodium Glutamate ◽  
Synthetic Media

A chemically defined medium for the growth of Rhizobium is described in which populations of up to 5 x 109 cells/ml were obtained. For the six strains of bacteria studied the complete medium supported exponential growth for two to five generations. The concentrations of biotin giving best growth varied ith strain between 125 and 250 f'g/l when the nitrogen source was sodium glutamate. NHt, NOs, and other amino acids, singly or in combination, did not upport as good growth as did sodium glutamate.

A chemically defined medium for the production of staphylococcal beta hemolysin

1973 ◽  
Vol 19 (10) ◽  
pp. 1319-1323 ◽  
Author(s):  
Brahma S. Sharma ◽  
Riaz-ul Haque
Keyword(s):  
Carbon Dioxide ◽  
Staphylococcus Aureus ◽  
Folic Acid ◽  
Sodium Citrate ◽  
Synthetic Medium ◽  
Defined Medium ◽  
Cell Yield ◽  
Good Growth ◽  
Chemically Defined Medium ◽  
Toxin Formation

A synthetic medium, containing L-proline, glycine, L-arginine, L-cystine, L-glutamine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, nicotinamide, thiamine, glucose, sodium citrate, Na2HPO4, KH2PO4, (NH4)2SO4, MgSO4∙7H2O, FeSO4∙7H2O, and CaCl2∙2H2O, was developed. It supported growth and beta hemolysin production by the 681C strain of Staphylococcus aureus when the culture was incubated under carbon dioxide. L-Threonine, L-tyrosine, i-inositol, folic acid, riboflavin, pyridoxal HCl, choline Cl, and D-Ca pantothenate were added for obtaining good growth and toxin formation under air. L-Proline, L-glutamine, and L-cystine were the absolute requirements for the production of beta hemolysin under carbon dioxide. Carbon dioxide stimulated the production of beta hemolysin, but invariably resulted in lower cell yield.

scholarly journals Transcriptional Response of Pasteurella multocida to Defined Iron Sources

2002 ◽  
Vol 184 (23) ◽  
pp. 6714-6720 ◽  
Author(s):  
Michael L. Paustian ◽  
Barbara J. May ◽  
Dongwei Cao ◽  
Daniel Boley ◽  
Vivek Kapur
Keyword(s):  
Gene Expression ◽  
Dna Microarrays ◽  
Pasteurella Multocida ◽  
Transcriptional Response ◽  
Global Gene Expression ◽  
Defined Medium ◽  
Ferric Citrate ◽  
Hypothetical Proteins ◽  
Chemically Defined Medium ◽  
Iron Source

ABSTRACT Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. This resulted in a set of data containing over 338,000 gene expression observations. On average, 12% of P. multocida genes were differentially expressed under any single condition. A majority of these genes encoded P. multocida proteins that were involved in either transport and binding or were annotated as hypothetical proteins. Several trends are evident when the data from different iron sources are compared. In general, only two genes (ptsN and sapD) were expressed at elevated levels under all of the conditions tested. The results also show that genes with increased expression in the presence of hemoglobin did not respond to transferrin or ferritin as an iron source. Correspondingly, genes with increased expression in the transferrin and ferritin experiments were expressed at reduced levels when hemoglobin was supplied as the sole iron source. Finally, the data show that genes that were most responsive to the presence of ferric citrate did not follow a trend similar to that of the other iron sources, suggesting that different pathways respond to inorganic or organic sources of iron in P. multocida. Taken together, our results demonstrate that unique subsets of P. multocida genes are expressed in response to different iron sources and that many of these genes have yet to be functionally characterized.

Physiology of two monocentric chytrids: comparative nutritional studies of Entophlyctis sp. and Entophlyctis aureus (Chytridiales)

2000 ◽  
Vol 78 (1) ◽  
pp. 105-109 ◽  
Author(s):  
Mozaffar W Hassan ◽  
Edward J Catapane
Keyword(s):  
Amino Acids ◽  
Nitrate Nitrogen ◽  
Defined Medium ◽  
Physiological Characteristics ◽  
Good Growth ◽  
Chemically Defined Medium ◽  
Vitamin Requirements ◽  
Nutritional Studies ◽  
Vitamin Mixture ◽  
Nutrient Solutions

This paper describes physiological characteristics of Entophlyctis sp. and Entophlyctis aureus Fisher. The two chytrids grew best at 20-25°C in a chemically defined medium, and at 20-30°C in nutrient solutions containing bactotryptone and glucose. The range of pH that supported good growth was 6.5-8.5. Both organisms utilized ammonium and nitrate nitrogen, several amino acids, and glucose, fructose, mannose, maltose, and raffinose. They were prototrophic with respect to vitamin requirements, and vitamin mixture at a concentration of 10 µg/mL inhibited growth. They are physiologically similar to Entophlyctis confervae-glomeratae (Cienkowski) Sparrow.Key words: Entophlyctis sp., Entophlyctis aureus, Entophlyctis confervae-glomeratae.

CHEMICALLY DEFINED MEDIUM FOR GROWTH OF MICROCOCCUS LYSODEIKTICUS

1961 ◽  
Vol 7 (1) ◽  
pp. 27-32 ◽  
Author(s):  
E. A. Grula ◽  
Shing-kei Luk ◽  
Yung-chieh Chu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Defined Medium ◽  
Good Growth ◽  
Chemically Defined Medium ◽  
Free Base ◽  
Specific Amino Acid ◽  
Absolute Requirement ◽  
Adenylic Acid ◽  
Amino Acid Requirements

A chemically defined medium for growth of M. lysodeikticus is presented. The organism possesses a relatively nonspecific but absolute purine requirement that can best be satisfied by the free base hypoxanthine although adenine also allows some growth. A substitution for hypoxanthine, however, can be made by inosine, adenosine, or adenylic acid, but not by guanosine or guanylic acid. Although biotin stimulates growth, equally good growth occurs using biocytin or biotiu-d-sulphoxide. Less stimulation is apparent using desthiobiotin, dl-oxybiotin, or biotirt-l-sulphoxide. Although amino acids are necessary for growth, no absolute requirement for a specific amino acid can be demonstrated. The amino acid requirements need to be defined in terms of those amino acids which support good growth in the presence or absence of glutamic acid.

Improved cryopreservation of domestic cat sperm in a chemically defined medium

2012 ◽  
Vol 78 (9) ◽  
pp. 2120-2128 ◽  
Author(s):  
M.M. Vick ◽  
H.L. Bateman ◽  
C.A. Lambo ◽  
W.F. Swanson
Keyword(s):  
Defined Medium ◽  
Domestic Cat ◽  
Chemically Defined Medium
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