STUDIES ON THE ANTIGENIC STRUCTURE OF HISTOPLASMA CAPSULATUM

1957 ◽  
Vol 3 (7) ◽  
pp. 975-985 ◽  
Author(s):  
N. A. Labzoffsky ◽  
J. B. Fischer ◽  
J. J. Hamvas

Employing physical and chemical methods eight antigenic fractions were isolated from Histoplasma capsulatum as determined by complement fixation technique. Two of the fractions were found to cross-react with coccidioidal antisera, two with coccidioidal and Blastomyces antisera, one with Blastomyces antisera, while the remaining three displayed specificity by reacting with Histoplasma antisera only. Some evidence is presented to indicate that the isolated fractions are antigenically distinct.

1959 ◽  
Vol 5 (4) ◽  
pp. 395-403 ◽  
Author(s):  
N. A. Labzoffsky ◽  
E. Kuitunen ◽  
L. P. Morrissey ◽  
J. J. Hamvas

By means of physical and chemical procedures seven antigenic fractions have been isolated from Trichinella spiralis larvae, as determined by complement fixation. Some evidence is presented to indicate that at least some of the isolated fractions are antigenically distinct and that antibodies in Trichinella-infested rabbit develop at different times for different fractions. Importance of using a properly selected antigen in serodiagnosis of trichinosis is discussed.


1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S13-S30 ◽  
Author(s):  
W. R. Butt

ABSTRACT Several chemical differences between FSH, LH and HCG have been reported: thus LH and HCG are richer in proline than FSH and FSH and HCG contain more N-acetyl neuraminic acid than LH. Sub-units of LH are formed by treatment with urea, guanidine or acid. HCG also may contain two sub-units. The sub-units from LH are biologically inert but retain their immunological activity: biological activity is restored when the sub-units are incubated together. There is much evidence from chemical and enzymic reactions that antigenic groups are distinct from those parts of the molecule essential for biological activity. N-acetyl neuraminic acid and probably other carbohydrates in FSH and HCG are not involved in immunological activity but are necessary for biological activity. Histidine, methionine and possibly cysteine appear to be essential for biological but not immunological activity of FSH, while tryptophan and possibly tyrosine are not essential for either. A few highly specific antisera to gonadotrophins have been prepared in rabbits and guinea pigs to crude antigens: there is no evidence that purified antigens are more likely to produce specific antisera. Differences in the immunological reactivities of urinary compared with pituitary gonadotrophins have been observed both by radioimmunoassay and by the complement fixation technique. The latter may be particularly useful for detecting structural differences in the hormones.


2016 ◽  
Vol 20 (17) ◽  
pp. 1797-1812 ◽  
Author(s):  
Xiaoyue Yu ◽  
Cuie Tang ◽  
Shanbai Xiong ◽  
Qijuan Yuan ◽  
Zhipeng Gu ◽  
...  

Author(s):  
Geetanjali Singh ◽  
Pramod Kumar Sharma ◽  
Rishabha Malviya

Aim/Objective: The author writes the manuscript by reviewing the literatures related to the biomedical application of metallic nanoparticles. The term metal nanoparticles are used to describe the nanosized metals with the dimension within the size range of 1-100 nm. Methods: The preparation of metallic nanoparticles and their application is an influential area for research. Among various physical and chemical methods (viz. chemical reduction, thermal decomposition, etc.) for synthesizing silver nanoparticles, biological methods have been suggested as possible eco-friendly alternatives. The synthesis of metallic nanoparticles is having many problems inclusive of solvent toxicity, the formation of hazardous byproducts and consumption of energy. So it is important to design eco-friendly benign procedures for the synthesis of metallic nanoparticles. Results: From the literature survey, we concluded that metallic nanoparticles have applications in the treatment of different diseases. Metallic nanoparticles are having a great advantage in the detection of cancer, diagnosis, and therapy. And it can also have properties such as antifungal, antibacterial, anti-inflammatory, antiviral and anti-angiogenic. Conclusion: In this review, recent upcoming advancement of biomedical application of nanotechnology and their future challenges has been discussed.


2021 ◽  
Vol 667 (1) ◽  
pp. 012015
Author(s):  
I Sulaiman ◽  
C Annisa ◽  
Y M Lubis ◽  
Z F Rozali ◽  
S Noviasari ◽  
...  

1971 ◽  
Vol 51 (3) ◽  
pp. 579-589 ◽  
Author(s):  
J. M. BAILLARGEON ◽  
J. P. LEMAY ◽  
W. B. HOLTMANN ◽  
L. A. CHARETTE

An experiment was conducted to compare various physical and chemical methods of castration of lambs at 15 and 60 days of age. Their effects on body growth, feed efficiency, dressing percentage and profitability were studied. At birth 100 lambs were divided among the 16 different treatments according to sex and the following breeding groups: Suffolk, North Country Cheviot, two-breed cross and three-breed cross. The different methods of castration were: complete removal of the testicles, Burdizzo, elastrator and partial castration. The sterilization methods consisted of intramuscular, intratesticular and intraveinous injection of cadmium chloride. All lambs were weaned at 30 days of age and placed in individual pens. They were subjected to similar environmental conditions and shipped to slaughter at approximately 45 kg body weight. Significant differences were observed among breeds, sexes and the various castration methods for daily gain, feed efficiency, days required to reach market and profitability. Age at castration did not have any important effect ort the variables measured. Superior gains, feed efficiency and profitability were observed for the partially castrated group, for the lambs that received an intramuscular injection of cadmium chloride and for the non-castrated males. All treatment groups involving males were superior to the females for the characters studied except for dressing percentage.


Author(s):  
Р.С. Решетова ◽  
О.Ю. Бганцева

Разработана технологическая схема получения жидкого сахара из полупродуктов свеклосахарного производства, которая предусматривает получение исходного (концентрированного) сахарного раствора из сиропа после выпарки, некондиционного сахара и желтого сахара последнего продукта с содержанием сухих веществ (СВ) от 68 до 72. Изучено изменение количества микроорганизмов в жидком сахаре в течение 30 сут хранения в закрытых сосудах при температуре окружающей среды без предварительной обработки физическим и химическим способами с целью использования сиропа при производстве пищевых продуктов. Установлено, что в растворе с содержанием СВ 68 72 количество микроорганизмов увеличилось: бактерий с 15 до 60 КОЕ/г, дрожжей и плесневых грибов с 10 до 34 КОЕ/г, что не превышает допустимых уровней СанПиН 2.3.2.1078 01 и ГОСТ Р 53035 2008. Определена длительность хранения жидкого сахара для использования в пищевых производствах не более 15 сут. The technological scheme of obtaining liquid sugar from semiproducts of sugar beet production, which provides for obtaining the initial (concentrated) sugar solution from the syrup after evaporation, substandard sugar and yellow sugar of the last product with a content of dry matters from 68 to 72, has been developed. The change in the number of microorganisms in liquid sugar was studied during 30 days of storage in closed vessels at ambient temperature without preliminary treatment by physical and chemical methods with the aim of using syrup in food production. It was found that in the solution with the content of dry matters 68 72 the number of microorganisms increased: bacteria from 15 to 60 CFU/g, yeast and fungi from 10 to 34 CFU/g, which does not exceed the permissible levels of SanPiN 2.3.2.1078 01 and GOST R 53035 2008. The storage duration of liquid sugar for use in food production was determined no more than 15 days.


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