MICROBIOLOGICAL FORMATION OF VITAMIN B12: II. SYNTHESIS OF VITAMIN B12-ACTIVE SUBSTANCES BY BACTERIA ISOLATED FROM CLAMS

1957 ◽  
Vol 3 (2) ◽  
pp. 195-202 ◽  
Author(s):  
B. A. Southcott ◽  
H. L. A. Tarr
Keyword(s):  
Vitamin B12 ◽  
Phosphate Buffer ◽  
Marine Invertebrates ◽  
Marked Decrease ◽  
Bacterial Flora ◽  
Vitamin B ◽  
Bacterial Cells ◽  
Factor B ◽  
Washed Cell ◽  
Active Substances

Vitamin B122occurred in certain seaweeds and this vitamin and other cobalamins were demonstrated regularly in marine invertebrates. A Bacillus and a Micrococcus isolated from fresh clams were selected for detailed study on the basis of their pronounced ability to form cobalamins, and because they appeared to be typical of the bacterial flora of these invertebrates. The Bacillus formed vitamin B12and other cobalamins, and this activity was stimulated by addition of Factor B and of certain purines or benziminazoles to the medium. With both organisms, 6-nitrobenziminazole proved particularly effective in stimulating formation of both vitamin B12and of other cobalamins, and Factor B stimulated this activity. Formation of cobalamins was associated with a marked decrease in Factor B. The cobalamins were associated with the bacterial cells, and no cobalamin synthesis by washed cell suspensions in phosphate buffer was observed.

The Distribution of Vitamin B12-Active Substances in some Marine Invertebrates of British Columbia

1952 ◽  
Vol 9 (3) ◽  
pp. 164-168 ◽  
Author(s):  
B. E. Maxwell
Keyword(s):  
British Columbia ◽  
Vitamin B12 ◽  
Marine Invertebrates ◽  
Quantitative Estimation ◽  
Microbiological Assay ◽  
Vitamin B ◽  
Its Phylogeny ◽  
Definite Relationship ◽  
Active Substances ◽  
Assay Technique

Quantitative estimation of the amounts of vitamin B12-active substances in some marine invertebrates was made using a microbiological assay technique. In general, species from phylum Mollusca were found to be richer in these substances than were species from phyla Echinodermata and Annelida and class Crustacea. No definite relationship was found to exist between the content of vitamin B12-active substances in an animal and its phylogeny.

scholarly journals Molecularly imprinted membrane for transport of urea, creatinine, and vitamin B12 as a hemodialysis candidate membrane

2021 ◽  
Vol 19 (1) ◽  
pp. 806-817
Author(s):  
Muhammad Cholid Djunaidi ◽  
Nabilah Anindita Febriola ◽  
Abdul Haris
Keyword(s):  
Polyethylene Glycol ◽  
Vitamin B12 ◽  
Kidney Function ◽  
Cross Linking ◽  
Vitamin B ◽  
Molecularly Imprinted ◽  
Selective Transport ◽  
Molecularly Imprinted Membrane ◽  
Better Than ◽  
Imprinted Membrane

Abstract High levels of urea and creatinine in the blood are a sign of decreased kidney function. To remove these substances from the blood, hemodialysis which utilizes membranes could be used. In this study, a molecularly imprinted membrane (MIM) was synthesized for the selective transport of urea. The synthesis is initiated with the polymerization of eugenol into polyeugenol and then into polyeugenoxy acetate (PA). The PA is then contacted with urea and then used as the functional polymer in the synthesis of MIM with polysulfone as the membrane base, and polyethylene glycol as the cross-linking agent. The result was later analyzed with FTIR and SEM-EDX. The membrane is then used in the transport of urea, creatinine, and vitamin B12 and then compared with the non-imprinted membrane (NIM) performance. By using UV-Vis spectrophotometry, the results showed that the membrane with 10 h heating variation is able to transport more urea and is more selective than NIM; this proves that the urea template on the MIM enables it to recognize urea molecules better than creatinine and vitamin B12. The order of transport from the best results is urea > creatinine > vitamin B12.

Röntgenspektroskopische Bestimmung der Wertigkeit des Kobalts im Vitamin B12

1954 ◽  
Vol 9 (8) ◽  
pp. 509-513 ◽  
Author(s):  
G. Boehm ◽  
A. Faessler ◽  
G. Rittmayer
Keyword(s):  
Vitamin B12 ◽  
Vitamin B

Mit einem fokussierenden Spektrographen großer Dispersion wird die Lage der K-Röntgen-absorptionskante des Kobalts in verschiedenen Verbindungen untersucht. Die Ergebnisse zeigen, daß es möglich ist, durch Bestimmung der Kantenlage die Wertigkeit des Kobalts in einer Verbindung zu bestimmen. Die Methode gestattet den eindeutigen Nachweis, daß das Kobalt im Vitamin B12 dreiwertig ist.

scholarly journals Effect of Vitamin-B1 and Vitamin-B12 on the Growth and Carotenoid Content of Haematococcus pluvialis CH-1

2015 ◽  
Vol 7 (9) ◽  
pp. 709-711
Author(s):  
Li-Xin Li ◽  
Zhi-Wei Song ◽  
Shun-Shan Duan ◽  
Ke-Jie Luo ◽  
Ting Li ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Haematococcus Pluvialis ◽  
Carotenoid Content ◽  
Vitamin B

NUTRITION OF A COBALAMIN-REQUIRING SOIL BACTERIUM

1957 ◽  
Vol 3 (2) ◽  
pp. 329-334 ◽  
Author(s):  
Milton K. Goldberg ◽  
S. H. Hutner ◽  
J. E. Ford
Keyword(s):  
Growth Response ◽  
Soil Bacterium ◽  
Intrinsic Activity ◽  
Vitamin B ◽  
Factor B ◽  
Naturally Occurring ◽  
High Concentrations

The pattern of the vitamin B12 requirement of a soil bacterium "Lochhead 38" (provisionally assigned to Arthrobacter) resembled that of the protozoan Ochromonas malhamensis and of higher animals. Of the naturally-occurring B12-vitamins, cyanocobalamin and vitamin B12III are active. Pseudovitamin B12 and Factor A have very little or no intrinsic activity, and when present in relatively high concentrations both compounds depress the rate of the growth response to limiting cyanocobalamin. Factor B, the porphyrin-like nucleus of the vitamin B12 molecule without the nucleotide, is inactive, as are also methionine and deoxyribosides. A disadvantage in the use of Lochhead 38 for assay purposes is that in vitamin-B12-dehcient cultures the organisms flocculate.

Preparation of Cod Liver Residues and Vitamin B12 Concentrates

1954 ◽  
Vol 11 (4) ◽  
pp. 355-361 ◽  
Author(s):  
Beryl Truscott ◽  
D. G. Gage ◽  
P. L. Hoogland
Keyword(s):  
Vitamin B12 ◽  
Nutritive Value ◽  
Vitamin B ◽  
Sodium Bisulphite ◽  
Drum Drying ◽  
Different Types

As the first part of an investigation of the nutritive value of cod liver, two methods of preparation of cod liver residue were studied, as well as the effect of drum drying the residue, with and without the presence of sodium bisulphite, defatting the dried residue and extracting vitamin B12 from the dried residue.It was found that Vandenheuvel's method of preparation of residue produces very good results. The fresh residue could be dried without appreciable loss of vitamin B12 activity on a double drum dryer, operated with steam at 20 p.s.i.g. Sodium bisulphite afforded some protection during the drying. The most satisfactory results in defatting were obtained by extraction with dichloroethylene. Concentrates with vitamin B12 activity equivalent to 2–4 micrograms per milliliter were produced by extraction of dried, defatted residue with water and evaporation in vacuo.Five different types of residue were prepared for further studies.

scholarly journals The study of clinical profile and vitamin B 12 in dengue fever patients

2021 ◽  
Vol 12 (7) ◽  
pp. 47-51
Author(s):  
Mamatha Tittamegalapalya Ramalingaiah ◽  
Jeetendra Kumar Jogihalli Mood ◽  
Satyanarayana Narayanashetty ◽  
Rashmi Madappa Bhuvaneshappa
Keyword(s):  
Vitamin B12 ◽  
Hospital Stay ◽  
Dengue Fever ◽  
Platelet Transfusion ◽  
Clinical Profile ◽  
Study Patient ◽  
Vitamin B ◽  
Severe Thrombocytopenia ◽  
Duration Of Hospital Stay ◽  
Vitamin B 12

Background: Dengue is an acute infectious febrile illness characterised by thrombocytopenia and platelet dysfunction leading to bleeding manifestations. Vitamin B12 is required for platelet production in the bone marrow. So, deficiency of vitamin B12 in dengue patients can have severe thrombocytopenia. Aims and Objective: Study was aimed to know the clinical profile of dengue fever patients and to correlate serum vitamin B12 level with severity of thrombocytopenia, platelet transfusion and duration of hospital stay. Materials and Methods: This observational study was done on dengue patients for period of 3 months January 2020 to March 2020. Confirmed cases of dengue fever with NS 1 Ag positive & Ig M antibody positive were included in the study. Patient with sepsis, underlying malignancy, autoimmune disorder, hematological disorder, drugs causing thrombocytopenia were excluded from the study. Appropriate statistical methods were applied. Results: Total 50 subjects were included. Majority of subjects were in the age group 31 to 40 years. 50% were males and females respectively. Most common clinical feature was fever and bleeding manifestations. Mean Vitamin B12 was significantly lower among those with severe thrombocytopenia and highest among those with no thrombocytopenia., there was significant difference in mean Platelet transfusion and duration of hospital stay with respect to severity of Platelet count. Conclusion: Dengue fever patients with vitamin B 12 deficiency had moderate to severe thrombocytopenia and more bleeding manifestations. Those patients required more platelet transfusion and increased duration of hospital stay.

Vitamin B12 levels in drug-treated bacterial cells

1975 ◽  
Vol 24 (13-14) ◽  
pp. 1359-1361
Author(s):  
Hanna Gajcy
Keyword(s):  
Vitamin B12 ◽  
Bacterial Cells

Increased intake of vitamin B12, folate, and omega-3 fatty acids to improve cognitive performance in offspring born to rats with induced hypertension during pregnancy

2018 ◽  
Vol 9 (7) ◽  
pp. 3872-3883 ◽  
Author(s):  
Nisha Kemse ◽  
Anvita Kale ◽  
Preeti Chavan-Gautam ◽  
Sadhana Joshi
Keyword(s):  
Fatty Acids ◽  
Folic Acid ◽  
Docosahexaenoic Acid ◽  
Vitamin B12 ◽  
Cognitive Performance ◽  
Vitamin B ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Induced Hypertension

Vitamin B12, folic acid, and docosahexaenoic acid levels are reported to be altered in women with preeclampsia.

scholarly journals Bioshell Calcium Oxide-Containing Liquids as a Sanitizer for the Reduction of Histamine Production in Raw Japanese Pilchard, Japanese Horse Mackerel, and Chub Mackerel

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 964
Author(s):  
Sumiyo Hiruma ◽  
Masayuki Ishihara ◽  
Shingo Nakamura ◽  
Yoko Sato ◽  
Haruka Asahina ◽  
...  
Keyword(s):  
Calcium Oxide ◽  
Pure Water ◽  
Bacterial Flora ◽  
Colloidal Dispersion ◽  
Coliform Bacteria ◽  
Horse Mackerel ◽  
Chub Mackerel ◽  
Bacterial Cells ◽  
Histamine Production ◽  
Fish Samples

Recently, there has been an increase in the number of food poisoning cases associated with histamine in food, mainly in relation to histamine in fish. Here, we investigated methods to decrease histamine levels in Japanese pilchard, Japanese horse mackerel, and chub Mackerel, stored at 10 °C using various concentrations of heated scallop bioshell calcium oxide (BiSCaO) suspension, dispersion (BiSCaO + Na2HPO4), colloidal dispersion (BiSCaO + NapolyPO4), scallop shell powder (SSP) Ca(OH)2 in pure water (PW) or saline, and BiSCaO water. BiSCaO in a high alkaline pH solution chemically decomposes histamine poorly, but the partial flocculation/precipitation of histamine was observed with 1 and 0.2 wt.% BiSCaO dispersion and BiSCaO colloidal dispersion, respectively. Cleaning fish samples with BiSCaO suspension, dispersion, colloidal dispersion, or BiSCaO water remarkably reduced histamine levels and normal bacterial flora (coliform bacteria (CF) and total viable bacterial cells (TC)) after storage for four days at 10 °C, while much higher histamine levels were observed after cleaning with saline. These results suggest that cleaning fish with BiSCaO dispersion, colloidal dispersion, or BiSCaO water can significantly reduce histamine levels through their bactericidal activity against histamine-producing bacteria.

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