Leptin Is an Important Endocrine Player That Directly Activates Gonadotropic Cells in Teleost Fish, Chub Mackerel

Leptin, secreted by adipocytes, directly influences the onset of puberty in mammals. Our previous study showed that leptin stimulation could promote the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from pituitary cells in primary culture and ovarian development in chub mackerel. This study aimed to elucidate the detailed mechanism of leptin-induced effects on gonadotropin hormone-producing cells. We produced recombinant leptin using silkworm pupae and investigated the effects of leptin on FSH and LH secretion and gene expression in the primary culture of pituitary cells from chub mackerel. The presence or absence of co-expression of lepr mRNA, FSH and LH b-subunit mRNA in gonadotropic cells was examined by double-labeled in situ hybridization. The addition of leptin significantly increased the secretion and gene expression of FSH and LH from male and female pituitary cells in primary culture. In contrast, gonadotropin-releasing hormone 1 affected neither FSH secretion in cells from females nor fshb and lhb expression in cells from males and females. The expression of lepr was observed in FSH- and LH-producing cells of both males and females. The results indicate that leptin directly regulates gonadotropin synthesis and secretion and plays an important role in the induction of puberty in teleost fish.

A genome assembly of the Atlantic chub mackerel (Scomber colias): a valuable teleost fishing resource

The Atlantic chub mackerel, Scomber colias Gmelin, 1789, is a medium-size pelagic fish with substantial importance in the fisheries of the Atlantic Ocean and the Mediterranean Sea. Over the past decade, this species has gained special relevance being one of the main targets of pelagic fisheries in the NE Atlantic. Here, we sequenced and annotated the first high-quality draft genome assembly of S. colias, produced with Pacbio HiFi long reads and Illumina Paired-End short reads. The estimated genome size is 814 Mb distributed into 2,028 scaffolds and 2,093 contigs with an N50 length of 4,19 and 3,34 Mb, respectively. We annotated 27,675 protein-coding genes and the BUSCO analyses indicated high completeness, with 97.3 % of the single-copy orthologs in the Actinopterygii library profile. The present genome assembly represents a valuable resource to address the biology and management of this relevant fishery. Finally, this is the fourth high-quality genome assembly within the Order Scombriformes and the first in the genus Scomber.

Distribution, Feeding Habits, and Growth of Chub Mackerel, Scomber japonicus, Larvae During a High-Stock Period in the Northern Satsunan Area, Southern Japan

To evaluate the importance of the northern Satsunan area in southern Japan as a spawning and nursery ground for chub mackerel (Scomber japonicus), we investigated the early life history characteristics (e.g., larval distribution, feeding habits, and growth) of S. japonicus over five successive years. This area is considered the main habitat and spawning ground of the congeneric species, S. australasicus. Using polymerase chain reaction-restriction fragment length polymorphism analysis, we first confirmed that S. japonicus larvae were abundant in the northern Satsunan area, potentially representing a major spawning and nursery ground in the Japanese Pacific coastal area. The number of recorded larvae started to increase in 2016, corresponding to the population dynamics of the Pacific stock of the species, which has shown increasing trends in recent years. Morphological and DNA metabarcoding analyses of gut contents and stable isotope analysis showed that, in addition to copepods, the larvae fed substantially on appendicularians. The trophic pathway involving appendicularians might support the feeding habits of S. japonicus, promoting its coexistence with other dominant species. Both the instantaneous growth rate and daily specific growth rate were comparable to those in the southern East China Sea, which is the main spawning and nursery ground of the species. Our data strongly suggest that the northern Satsunan area has favorable conditions for sustaining high larval population densities, even during phases with high population numbers. Our results provide insights for the fisheries management for S. japonicus in the Japanese Pacific coastal area, especially during high-stock periods.

Positive Effects of Fast Growth on Locomotor Performance in Pelagic Fish Juveniles: In Contrast to Existing Evidence

Many laboratory experiments on aquatic vertebrates that inhabit closed water or coastal areas have highlighted negative effects of fast growth on swimming performance. Nonetheless, field studies on pelagic fishes have provided evidence of survival advantages of faster growing individuals. To reconcile this contradiction, we examined the relationship between growth rate and swimming performance as a continuous function for juveniles of chub mackerel (scomber japonicus) using 3D tracking analysis. For experiments, 20, 24, 27 and 30 days-post-hatch individuals within the size range of 14.5–25.3 mm were used. We found that the growth–swimming (burst speed) relationship in chub mackerel was substantially positive and it was suggested to be supported by morphological traits such as muscle area, which also positively correlated with growth rate. This finding is consistent with field observations showing selective survival of fast-growing individuals of this species, reconciling the current contradiction between laboratory experiments and field observations. Growth was suggested to trade off with swimming performance, as reported in many previous studies, when it was extremely fast. Therefore, a dome-shaped quadratic curve described the relationship between growth rate and burst speed better than a linear or generalized linear model. These results, obtained from the rarely tested offshore species, strongly suggests the importance of experimental verification using animals that inhabit various types of habitats in understanding the principles underlying the evolution of growth–locomotor relationship.