RFLP analyses and segregation of molecular markers in plants produced by in vitro anther culture, selfing, and reciprocal crosses of two lines of self-incompatible Solanum chacoense

Genome ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 775-783 ◽  
Author(s):  
Sylvain R. Rivard ◽  
Marc K. Saba-El-Leil ◽  
Benoît S. Landry ◽  
Mario Cappadocia
Keyword(s):  
Anther Culture ◽  
Solanum Chacoense ◽  
Rflp Markers ◽  
Self Incompatibility ◽  
Distorted Segregation ◽  
Plant Populations ◽  
S Alleles ◽  
Parental Lines ◽  
Incompatibility Locus

RFLP analyses were used to characterize several plant populations of Solanum chacoense Bitt. developed to investigate the generation of new S alleles at the self-incompatibility locus. The plant material consisted of two diploid parental lines, their anther culture derived (AC) progenies, their selfed progenies, and their reciprocal F1 hybrids. The RFLP analyses on the AC plants (121 individuals in total) permitted unambiguous identification of their origin. In particular, a distinction between plants originated from reduced (n) or unreduced (2n) microspores could be made. All the AC plants produced by gametic embryogenesis showed distinct RFLP patterns, whereas a number of clones (i.e., plants with identical RFLP patterns) were found among those regenerated via callus. The analyses conducted on the selfed progenies (69 plants) and the F1 hybrids (66 plants) showed only one case of accidental outcross. Segregation studies of the RFLP markers revealed significant deviations from expected Mendelian ratios in both AC-derived populations, as well as in the selfed progenies. Such deviations, however, were rare in the reciprocal F1 hybrids. These results are discussed in relation to the possible presence of genetic sieves operating during AC, illegitimate selfing, or during normal fertilization.Key words: anther culture, RFLP markers, distorted segregation, self-incompatibility, Solanum chacoense, wild potato.

scholarly journals Identification and Characterization of a Polymorphic Receptor Kinase Gene Linked to the Self-Incompatibility Locus of Arabidopsis lyrata

Genetics ◽  
2001 ◽  
Vol 158 (1) ◽  
pp. 387-399 ◽  
Author(s):  
Mikkel H Schierup ◽  
Barbara K Mable ◽  
Philip Awadalla ◽  
Deborah Charlesworth
Keyword(s):  
Brassica Species ◽  
Self Incompatibility ◽  
Kinase Gene ◽  
Arabidopsis Lyrata ◽  
The Family ◽  
S Alleles ◽  
Sib Families ◽  
Identification And Characterization ◽  
Incompatibility Locus

Abstract We study the segregation of variants of a putative self-incompatibility gene in Arabidopsis lyrata. This gene encodes a sequence that is homologous to the protein encoded by the SRK gene involved in self-incompatibility in Brassica species. We show by diallel pollinations of plants in several full-sib families that seven different sequences of the gene in A. lyrata are linked to different S-alleles, and segregation analysis in further sibships shows that four other sequences behave as allelic to these. The family data on incompatibility provide evidence for dominance classes among the S-alleles, as expected for a sporophytic SI system. We observe no division into pollen-dominant and pollen-recessive classes of alleles as has been found in Brassica, but our alleles fall into at least three dominance classes in both pollen and stigma expression. The diversity among sequences of the A. lyrata putative S-alleles is greater than among the published Brassica SRK sequences, and, unlike Brassica, the alleles do not cluster into groups with similar dominance.

scholarly journals In vitro Androgenesis of Some Wheat (Triticum aestivum L.) Varieties and their F1, F2 Progenies and Combining Ability of Embryoid Production

2014 ◽  
Vol 18 (2) ◽  
pp. 57-60
Author(s):  
M Hasan ◽  
J Pauk ◽  
Z Kertesz
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Combining Ability ◽  
General Combining Ability ◽  
Polygenic Effect ◽  
Parental Lines ◽  
F2 Generation

Six parental lines along with their F1 and F2 progenies were investigated for general combining ability (GCA) effects on embryo-like-structure (ELS) production in anther culture. Significant differences were found among the parents in respect to the ELS production. Evaluating the combining ability effects, among the female parents Alfold showed the best result in ELS production, while Palma was the best among the male lines. While comparing F1 and F2 generations, in most of the cases (except one) F2 generation was superior in ELS production. Polygenic effect plays a key role in such type of superiority in the F2 generation. The reason for such superiority may be due to the recombination of superior genes.DOI: http://dx.doi.org/10.3329/pa.v18i2.18091 Progress. Agric. 18(2): 57 - 60, 2007

scholarly journals Production of inbred progenies of diploid potatoes using an S-locus inhibitor (Sli) gene, and their characterization

Genome ◽  
2000 ◽  
Vol 43 (3) ◽  
pp. 495-502 ◽  
Author(s):  
R K Birhman ◽  
K Hosaka
Keyword(s):  
Solanum Chacoense ◽  
Chromosome 12 ◽  
Rflp Markers ◽  
S Locus ◽  
Distorted Segregation ◽  
Wild Potato Species ◽  
Potato Genome ◽  
Self Compatibility ◽  
Sli Gene ◽  
S Locus Inhibitor

To develop inbred lines from self-incompatible, cultivated diploid potatoes, an S-locus inhibitor (Sli) gene derived from a self-compatible variant of a wild potato species, Solanum chacoense, was incorporated into various cultivated diploid potatoes. The progeny was selfed twice by the action of the Sli gene to obtain 74 S2 inbred clones belonging to 8 families. More than 40% of them were either non-flowering or pollen sterile. Among the pollen fertile clones, self-compatible clones occurred with a much lower frequency (20.9%) than expected (83.3%). The result demonstrated that self-compatibility was introduced and expressed in the gene pool of cultivated diploid potatoes by an action of the Sli gene, although serious inbreeding depression associated with selfing occurred. The genotypes of S2 inbreds were surveyed using 46 S. chacoense - specific RFLP (restriction fragment length polymorphism) markers covering the whole potato genome. More than half of the markers (67.4%) showed distorted segregation. Particularly, all markers on chromosome 12 were overrepresented in the S2 inbreds. This confirms our earlier finding that the Sli gene locates on chromosome 12 and the alleles linked with this gene are preferentially transmitted because of its essential requirement for selfing.Key words: RFLP markers, inbreds, diploid potato, distorted segregation.

Molecular Genetics and Biology of Self-Incompatibility in Nicotiana alata, an Ornamental Tobacco

1990 ◽  
Vol 17 (3) ◽  
pp. 345 ◽  
Author(s):  
BA Mcclure ◽  
V Haring ◽  
PR Ebert ◽  
MA Anderson ◽  
A Bacic ◽  
...  
Keyword(s):  
Amino Acid ◽  
Molecular Genetics ◽  
Amino Acid Sequences ◽  
Cysteine Residues ◽  
Nicotiana Alata ◽  
In Vitro Growth ◽  
Self Incompatibility ◽  
Glycosylation Sites ◽  
S Alleles

Glycoproteins are present in the styles of several self-incompatible species within the Solanaceae which segregate with particular alleles of the S-(self-incompatibility) gene. The amino acid sequences of style glycoproteins corresponding to different S-alleles of N. alata are homologous in some regions and variable in others. Homologous regions include N-terminal sequences as well as most of the glycosylation sites and cysteine residues. The isolated style S-glycoproteins inhibit in vitro growth of pollen tubes of several S-genotypes, with some specificity in the interaction. The isolated S-glycoproteins have ribonuclease activity which may be involved in their action in arrest of growth of incompatible (self) pollen.

scholarly journals Distorted segregation of RFLP markers in regenerated plants derived from anther culture of an Fl hybrid of rice.

1996 ◽  
Vol 71 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Masumi Yamagishi ◽  
Masahiro Yano ◽  
Yoshimichi Fukuta ◽  
Kiichi Fukui ◽  
Motoyasu Otani ◽  
...  
Keyword(s):  
Anther Culture ◽  
Rflp Markers ◽  
Distorted Segregation ◽  
Regenerated Plants
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