scholarly journals Distorted segregation of RFLP markers in regenerated plants derived from anther culture of an Fl hybrid of rice.

1996 ◽  
Vol 71 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Masumi Yamagishi ◽  
Masahiro Yano ◽  
Yoshimichi Fukuta ◽  
Kiichi Fukui ◽  
Motoyasu Otani ◽  
...  
Keyword(s):  
Anther Culture ◽  
Rflp Markers ◽  
Distorted Segregation ◽  
Regenerated Plants

RFLP analyses and segregation of molecular markers in plants produced by in vitro anther culture, selfing, and reciprocal crosses of two lines of self-incompatible Solanum chacoense

Genome ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 775-783 ◽  
Author(s):  
Sylvain R. Rivard ◽  
Marc K. Saba-El-Leil ◽  
Benoît S. Landry ◽  
Mario Cappadocia
Keyword(s):  
Anther Culture ◽  
Solanum Chacoense ◽  
Rflp Markers ◽  
Self Incompatibility ◽  
Distorted Segregation ◽  
Plant Populations ◽  
S Alleles ◽  
Parental Lines ◽  
Incompatibility Locus

RFLP analyses were used to characterize several plant populations of Solanum chacoense Bitt. developed to investigate the generation of new S alleles at the self-incompatibility locus. The plant material consisted of two diploid parental lines, their anther culture derived (AC) progenies, their selfed progenies, and their reciprocal F1 hybrids. The RFLP analyses on the AC plants (121 individuals in total) permitted unambiguous identification of their origin. In particular, a distinction between plants originated from reduced (n) or unreduced (2n) microspores could be made. All the AC plants produced by gametic embryogenesis showed distinct RFLP patterns, whereas a number of clones (i.e., plants with identical RFLP patterns) were found among those regenerated via callus. The analyses conducted on the selfed progenies (69 plants) and the F1 hybrids (66 plants) showed only one case of accidental outcross. Segregation studies of the RFLP markers revealed significant deviations from expected Mendelian ratios in both AC-derived populations, as well as in the selfed progenies. Such deviations, however, were rare in the reciprocal F1 hybrids. These results are discussed in relation to the possible presence of genetic sieves operating during AC, illegitimate selfing, or during normal fertilization.Key words: anther culture, RFLP markers, distorted segregation, self-incompatibility, Solanum chacoense, wild potato.

Anther culture of a 2n pollen producing clone of Solanum phureja Juz. &Buk.

1985 ◽  
Vol 27 (5) ◽  
pp. 559-564 ◽  
Author(s):  
R. E. Veilleux ◽  
J. Booze-Daniels ◽  
E. Pehu
Keyword(s):  
Anther Culture ◽  
Polyacrylamide Gel Electrophoresis ◽  
Pollen Grains ◽  
Unreduced Gametes ◽  
Solanum Phureja ◽  
2N Pollen ◽  
Regenerated Plants ◽  
Series Of Experiments ◽  
Pollen Formation ◽  
First And Second Generation

In a series of experiments, a total of 95 plants were regenerated from culture of 1416 anthers of a single genotype (PP5) of Solanum phureja that expressed a variable frequency of 2n pollen by the genetic equivalent of first division restitution. The regenerated plants included 29 monoploids (2n = x = 12), 58 diploids (2n = 2x = 24) and 8 tetraploids (2n = 4x = 48). Monoploids carrying the potential for 2n pollen formation are central to the development of a breeding scheme to construct highly heterozygous diplandroids. Segregation of tuber flesh color and tuber protein bands revealed by polyacrylamide gel electrophoresis in first and second generation anther-derived diploid plants provided evidence for both embryogenesis of 2n pollen as well as doubling of monoploid genomes as sources of anther-derived diploids. Because of variation for the same genetic markers in anther-derived tetraploids, sources other than embryogenesis of doubly restituted (4n) pollen grains were implied. An enhanced response to anther culture was noted in some anther-derived dihaploids.Key words: unreduced gametes, potato, anther culture, diplandroid, monoploid.

scholarly journals Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry

2014 ◽  
Vol 117 (3) ◽  
pp. 311-322 ◽  
Author(s):  
Edgardo Carloni ◽  
Andrea Ribotta ◽  
Eliana López Colomba ◽  
Sabrina Griffa ◽  
Mariana Quiroga ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Somatic Embryogenesis ◽  
Anther Culture ◽  
Buffel Grass ◽  
Regenerated Plants

Microsatellite marker analysis of an anther-derived potato family: skewed segregation and gene–centromere mapping

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 236-242 ◽  
Author(s):  
Eduard Chani ◽  
Varda Ashkenazi ◽  
Jossi Hillel ◽  
Richard E Veilleux
Keyword(s):  
Anther Culture ◽  
Segregation Distortion ◽  
Interspecific Hybrid ◽  
Genomic Library ◽  
Unreduced Gametes ◽  
Distorted Segregation ◽  
Backcross Population ◽  
Skewed Segregation ◽  
Ssr Loci ◽  
Simple Sequence

Segregation patterns of polymorphic simple sequence repeat (SSR) primer pairs were investigated in monoploid potato families derived from anther culture. A total of 14 primers developed from the sequences in the database, as well as from a genomic library of potato, was used. Distorted segregation was observed for seven (50%) polymorphic loci among monoploids derived from an interspecific hybrid. Similar distortion was observed for only one of five loci that could be contrasted between the two monoploid families. Segregation distortion was less common in the sexually derived backcross population between the interspecific hybrid and either of its parents. One locus could be putatively linked to a lethal allele because it showed distorted segregation in both monoploid families, a group of 70 heterozygous diploids derived from unreduced gametes through anther culture, and a backcross population. These diploids were used to map the polymorphic SSR markers with respect to the centromeres using half-tetrad analysis. The majority of the SSR loci mapped more than 33 cM from the centromere, suggesting the occurrence of a single crossover per chromosome arm.Key words: androgenesis, segregation distortion, simple sequence repeats (SSRs), Solanum phureja, unreduced gametes.

scholarly journals Production of New Regenerated Plants by Anther Culture of the Hybrids of Italian ryegrass X Tall fescue

2002 ◽  
Vol 22 (4) ◽  
pp. 273-278 ◽  
Author(s):  
K.Y. Kim ◽  
K.M. Kang ◽  
K.J. Choi ◽  
Y.S. Jang ◽  
Y.C. Lim ◽  
...  
Keyword(s):  
Anther Culture ◽  
Tall Fescue ◽  
Italian Ryegrass ◽  
Regenerated Plants

scholarly journals Production of inbred progenies of diploid potatoes using an S-locus inhibitor (Sli) gene, and their characterization

Genome ◽  
2000 ◽  
Vol 43 (3) ◽  
pp. 495-502 ◽  
Author(s):  
R K Birhman ◽  
K Hosaka
Keyword(s):  
Solanum Chacoense ◽  
Chromosome 12 ◽  
Rflp Markers ◽  
S Locus ◽  
Distorted Segregation ◽  
Wild Potato Species ◽  
Potato Genome ◽  
Self Compatibility ◽  
Sli Gene ◽  
S Locus Inhibitor

To develop inbred lines from self-incompatible, cultivated diploid potatoes, an S-locus inhibitor (Sli) gene derived from a self-compatible variant of a wild potato species, Solanum chacoense, was incorporated into various cultivated diploid potatoes. The progeny was selfed twice by the action of the Sli gene to obtain 74 S2 inbred clones belonging to 8 families. More than 40% of them were either non-flowering or pollen sterile. Among the pollen fertile clones, self-compatible clones occurred with a much lower frequency (20.9%) than expected (83.3%). The result demonstrated that self-compatibility was introduced and expressed in the gene pool of cultivated diploid potatoes by an action of the Sli gene, although serious inbreeding depression associated with selfing occurred. The genotypes of S2 inbreds were surveyed using 46 S. chacoense - specific RFLP (restriction fragment length polymorphism) markers covering the whole potato genome. More than half of the markers (67.4%) showed distorted segregation. Particularly, all markers on chromosome 12 were overrepresented in the S2 inbreds. This confirms our earlier finding that the Sli gene locates on chromosome 12 and the alleles linked with this gene are preferentially transmitted because of its essential requirement for selfing.Key words: RFLP markers, inbreds, diploid potato, distorted segregation.

scholarly journals Identification of Ploidy Level of the Regenerated Plants by Anther Culture in Rice.

1994 ◽  
Vol 44 (1) ◽  
pp. 19-22
Author(s):  
Kazuhiro Nakamura ◽  
Hiroaki Suzuki ◽  
Kazumi Hattori ◽  
Yuzo Futsuhara
Keyword(s):  
Anther Culture ◽  
Ploidy Level ◽  
Regenerated Plants

scholarly journals Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1064
Author(s):  
Piotr T. Bednarek ◽  
Renata Orłowska
Keyword(s):  
Anther Culture ◽  
De Novo ◽  
Silver Ions ◽  
Dna Demethylation ◽  
Induction Medium ◽  
Green Plants ◽  
Ion Concentrations ◽  
Regenerated Plants ◽  
Short Time

Plant anther culture allows for the regeneration of uniform and homozygous double haploids. However, off-type regenerants may appear as a result of so-called tissue culture-induced variation (TCIV). In addition, the presence of Cu2+ and Ag+ ions in the culture medium might influence the number of green plants. The regenerants were obtained via anther cultures of barley under varying Cu2+ and Ag+ ion concentrations in the induction medium during distinct time conditions. DArTseqMet markers were evaluated based on regenerants and donor plants and delivering data on DNA demethylation (DM) and de novo methylation (DNM) and changes in methylation (Delta). The number of green regenerated plants per 100 anthers (GPs) was evaluated. The Cu2+ and Ag+ ion concentrations moderated relationships between Delta and the number of green plants conditional on time of tissue cultures. Depending on the ions, moderated moderation is valid within the different time of anther culture. When the highest concentration of copper is analyzed, plant regeneration is possible under short ‘Time’ (21 days) of anther culture wherein Delta is negative or under elongated Time when Delta is positive. Under 21 days of culture, the highest concentration of silver ions and when Delta is negative, some regenerants could be evaluated. However, under high Ag+ concentration when Time of culture is long and Delta positive, the highest number of green plants could be obtained.

scholarly journals Successful Regeneration and Characterization of Anther Derived Rice Hybrid Plants from O. Sativa L. X O. Rufipogon Griff.

1970 ◽  
Vol 5 (5) ◽  
pp. 14-18 ◽  
Author(s):  
Bindeshwar Prasad Sah ◽  
Raj Kumar Niroula
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Albino Plants ◽  
Large Size ◽  
Hybrid Plants ◽  
Regenerated Plants ◽  
Scientific World ◽  
Haploid Population ◽  
Doubled Haploid Plants

Doubled haploid regenerated through anther culture of wide cross rice hybrids is one of the potential tools to produce various useful genetic and cytogenetic stocks for molecular studies. In this study, an interspecific hybrid derived from Oryza sativa and O. rufipogon was subjected to anther culture with the aim to regenerate doubled haploid population for their characterization. A total of 21 green and 31 albino plants were obtained from 1904 anthers of O. sativa cv. Himali x O. rufipogon. Six types of androclonal variants were identified based on morphology and panicle characteristics of regenerated plants. Among 21 plants, only six were found diploid with partially fertility. Despite the sterility and preponderance of wild traits, some of the plants showed useful traits such as increased in panicle and spikelet length, reduced shattering and awning, and large size anther as in O. rufipogon. Two of the regenerants were superior for panicle related traits over either parent. The genotyping study using SSR markers also revealed the substantial variation among regenerants. At least three allelic variants were recorded among individual plants based on our preliminary study. The regenerated doubled haploid plants showed large amount of useful variation both at phenotype and molecular level and they can be exploited to enrich the current rice gene pool. Key words: Anther culturability; Albino; Androclon; Calli; Meiosis; SSR marker. DOI: 10.3126/sw.v5i5.2649 Scientific World, Vol. 5, No. 5, July 2007 14-18

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