Restriction fragment length polymorphism (RFLP) analyses of plants produced by in vitro anther culture of Solanum chacoense Bitt

1989 ◽  
Vol 78 (1) ◽  
pp. 49-56 ◽  
Author(s):  
S. R. Rivard ◽  
M. Cappadocia ◽  
G. Vincent ◽  
N. Brisson ◽  
B. S. Landry
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Anther Culture ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Solanum Chacoense ◽  
Restriction Fragment Length

scholarly journals Restriction Fragment Length Polymorphism Analysis of Anther-culture-derived Solanum chacoense

HortScience ◽  
1994 ◽  
Vol 29 (3) ◽  
pp. 206-208 ◽  
Author(s):  
Ram K. Birhman ◽  
Sylvain R. Rivard ◽  
Mario Cappadocia
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Anther Culture ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Solanum Chacoense ◽  
Polymorphism Analysis ◽  
Restriction Fragment Length

Using restriction fragment length polymorphism (RFLP) analysis, the genetic architecture of some anther-culture-derived S. chacoense Bitt. plants was studied, and their origins were elucidated. Our RFLP analyses showed that 1) several plants, even of different ploidy but otherwise genetically identical (clones), can be regenerated from callus originating from a single microspore and, conversely, that 2) some plants regenerated from single callus can have different genetic constitutions and, therefore, must have originated from two different microspore. These findings imply that previous anther culture efficiency estimates might have to be reconsidered.

scholarly journals Selection of Enzymes for Terminal Restriction Fragment Length Polymorphism Analysis of Fungal Internally Transcribed Spacer Sequences

2009 ◽  
Vol 75 (14) ◽  
pp. 4747-4752 ◽  
Author(s):  
Pablo Alvarado ◽  
Jose L. Manjón
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Fragment Size ◽  
Polymorphism Analysis ◽  
Internally Transcribed Spacer ◽  
Restriction Fragment Length

ABSTRACT Terminal restriction fragment length polymorphism (TRFLP) profiling of the internally transcribed spacer (ITS) ribosomal DNA of unknown fungal communities is currently unsupported by a broad-range enzyme-choosing rationale. An in silico study of terminal fragment size distribution was therefore performed following virtual digestion (by use of a set of commercially available 135 type IIP restriction endonucleases) of all published fungal ITS sequences putatively annealing to primers ITS1 and ITS4. Different diversity measurements were used to rank primer-enzyme pairs according to the richness and evenness that they showed. Top-performing pairs were hierarchically clustered to test for data dependency. The enzyme set composed of MaeII, BfaI, and BstNI returned much better results than randomly chosen enzyme sets in computer simulations and is therefore recommended for in vitro TRFLP profiling of fungal ITSs.

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