scholarly journals Production of inbred progenies of diploid potatoes using an S-locus inhibitor (Sli) gene, and their characterization

Genome ◽  
2000 ◽  
Vol 43 (3) ◽  
pp. 495-502 ◽  
Author(s):  
R K Birhman ◽  
K Hosaka
Keyword(s):  
Solanum Chacoense ◽  
Chromosome 12 ◽  
Rflp Markers ◽  
S Locus ◽  
Distorted Segregation ◽  
Wild Potato Species ◽  
Potato Genome ◽  
Self Compatibility ◽  
Sli Gene ◽  
S Locus Inhibitor

To develop inbred lines from self-incompatible, cultivated diploid potatoes, an S-locus inhibitor (Sli) gene derived from a self-compatible variant of a wild potato species, Solanum chacoense, was incorporated into various cultivated diploid potatoes. The progeny was selfed twice by the action of the Sli gene to obtain 74 S2 inbred clones belonging to 8 families. More than 40% of them were either non-flowering or pollen sterile. Among the pollen fertile clones, self-compatible clones occurred with a much lower frequency (20.9%) than expected (83.3%). The result demonstrated that self-compatibility was introduced and expressed in the gene pool of cultivated diploid potatoes by an action of the Sli gene, although serious inbreeding depression associated with selfing occurred. The genotypes of S2 inbreds were surveyed using 46 S. chacoense - specific RFLP (restriction fragment length polymorphism) markers covering the whole potato genome. More than half of the markers (67.4%) showed distorted segregation. Particularly, all markers on chromosome 12 were overrepresented in the S2 inbreds. This confirms our earlier finding that the Sli gene locates on chromosome 12 and the alleles linked with this gene are preferentially transmitted because of its essential requirement for selfing.Key words: RFLP markers, inbreds, diploid potato, distorted segregation.

RFLP analyses and segregation of molecular markers in plants produced by in vitro anther culture, selfing, and reciprocal crosses of two lines of self-incompatible Solanum chacoense

Genome ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 775-783 ◽  
Author(s):  
Sylvain R. Rivard ◽  
Marc K. Saba-El-Leil ◽  
Benoît S. Landry ◽  
Mario Cappadocia
Keyword(s):  
Anther Culture ◽  
Solanum Chacoense ◽  
Rflp Markers ◽  
Self Incompatibility ◽  
Distorted Segregation ◽  
Plant Populations ◽  
S Alleles ◽  
Parental Lines ◽  
Incompatibility Locus

RFLP analyses were used to characterize several plant populations of Solanum chacoense Bitt. developed to investigate the generation of new S alleles at the self-incompatibility locus. The plant material consisted of two diploid parental lines, their anther culture derived (AC) progenies, their selfed progenies, and their reciprocal F1 hybrids. The RFLP analyses on the AC plants (121 individuals in total) permitted unambiguous identification of their origin. In particular, a distinction between plants originated from reduced (n) or unreduced (2n) microspores could be made. All the AC plants produced by gametic embryogenesis showed distinct RFLP patterns, whereas a number of clones (i.e., plants with identical RFLP patterns) were found among those regenerated via callus. The analyses conducted on the selfed progenies (69 plants) and the F1 hybrids (66 plants) showed only one case of accidental outcross. Segregation studies of the RFLP markers revealed significant deviations from expected Mendelian ratios in both AC-derived populations, as well as in the selfed progenies. Such deviations, however, were rare in the reciprocal F1 hybrids. These results are discussed in relation to the possible presence of genetic sieves operating during AC, illegitimate selfing, or during normal fertilization.Key words: anther culture, RFLP markers, distorted segregation, self-incompatibility, Solanum chacoense, wild potato.

scholarly journals Ubiquitination of S4-RNase by S-LOCUS F-BOX LIKE2 Contributes to Self-Compatibility of Sweet Cherry ‘Lapins’

2020 ◽  
Vol 184 (4) ◽  
pp. 1702-1716
Author(s):  
Yang Li ◽  
Xuwei Duan ◽  
Chuanbao Wu ◽  
Jie Yu ◽  
Chunsheng Liu ◽  
...  
Keyword(s):  
Sweet Cherry ◽  
S Locus ◽  
Self Compatibility

scholarly journals An S-Locus Independent Pollen Factor Confers Self-Compatibility in ‘Katy’ Apricot

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53947 ◽  
Author(s):  
Elena Zuriaga ◽  
Juan V. Muñoz-Sanz ◽  
Laura Molina ◽  
Ana D. Gisbert ◽  
María L. Badenes ◽  
...  
Keyword(s):  
S Locus ◽  
Self Compatibility

Breakdown of a hybridization barrier between Solanum pinnatisectum Dunal and potato using the S locus inhibitor gene (Sli)

Euphytica ◽  
2014 ◽  
Vol 197 (1) ◽  
pp. 119-132 ◽  
Author(s):  
Rena Sanetomo ◽  
Seishi Akino ◽  
Nobuyuki Suzuki ◽  
Kazuyoshi Hosaka
Keyword(s):  
S Locus ◽  
Inhibitor Gene ◽  
S Locus Inhibitor

scholarly journals How does the S-locus determining self-incompatibility in stone fruits work in self-compatible peach?

2005 ◽  
pp. 93-100
Author(s):  
Attila Hegedűs ◽  
Júlia Halász ◽  
Zoltán Szabó ◽  
József Nyéki ◽  
Andrzej Pedryc
Keyword(s):  
Prunus Persica ◽  
Fruit Trees ◽  
S Locus ◽  
Recognition Mechanism ◽  
Specific Pcr ◽  
Fruit Species ◽  
Self Fertilization ◽  
Self Compatibility ◽  
S Allele ◽  
Available Information

The majority of stone fruit species are self-incompatible, a feature that is determined by a specific recognition mechanism between the S-ribonuclease enzymes residing in the pistils and the F-box proteins expressed in the pollen tubes. Failure in the function of any component of this bipartite system resulted in self-compatibility (SC) in many cultivars of Prunus species. Peach (Prunus persica (L.) Batsch.) is the only species in the Prunoideae subfamily that is traditionally known to be self-compatible, but its molecular background is completely unknown. Isoelectric focusing and S-gene specific PCR revealed that SC is not due to functional inability of pistil ribonucleases. We hypothesize that SC may be a consequence of a kind of pollen-part mutation or the action of one or more currently unknown modifier gene(s). Only two S-alleles were identified in a set of peach genotypes of various origin and phenotypes in contrast to the 17–30 alleles described in self-incompatible fruit trees. Most important commercial cultivars carry the same S-allele and are in a homozygote state. This indicates the common origin of these cultivars and also the consequence of self-fertilization. According to the available information, this is the first report to elucidate the role of S-locus in the fertilization process of peach. 

scholarly journals Characterization of S haplotype in a new self-compatible Brassica rapa cultivar Dahuangyoucai

2013 ◽  
Vol 49 (No. 4) ◽  
pp. 157-163 ◽  
Author(s):  
X. Zhang ◽  
C. Ma ◽  
D. Yin ◽  
W. Zhu ◽  
C. Gao ◽  
...  
Keyword(s):  
Brassica Species ◽  
Functional Class ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility ◽  
Female Determinant ◽  
First Time ◽  
S Locus Glycoprotein ◽  
Signalling Cascade

The most important Brassica species, B. rapa, is naturally self-incompatible. Self-compatible mutants would be useful for dissecting the molecular mechanism of self-incompatibility (SI), a process that promotes outcrossing by recognizing and refusing self-pollens. The S haplotype in a new self-compatible B. rapa cultivar, Dahuangyoucai, was characterized for the first time in this study. Sequence analysis of the S-locus genes, SLG (S-locus glycoprotein), SRK (S-locus receptor kinase) and SCR (S-locus cysteine-rich protein) revealed that Dahuangyoucai contained S haplotype highly similar to S-f2, a non-functional class I S haplotype identified in another self-compatible B. rapa cultivar, Yellow Sarson. Mutations of MLPK (M-locus protein kinase) and non-transcription of the male determinant, SCR, were observed in this cultivar, which is similar to the situation reported in Yellow Sarson. With respect to the female determinant, SRK, no transcript was detected in Yellow Sarson but two fragments were detected in Dahuangyoucai. One fragment was highly similar to SRK-f2, but the other fragment was different from the signal factors previously identified in the SI reaction. The results suggest that Dahuangyoucai and Yellow Sarson have the same origin and a similar mechanism of self-compatibility, but diverge after mutations in SRK, SCR and MLPK. Further studying the self-compatibility of Dahuangyoucai might identify novel factors involved in the SI signalling cascade and provide new insights into the mechanisms of SI in Brassicaceae.

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