LACTATE DEHYDROGENASE IN RABBIT OOCYTES AND EGGS

1978 ◽  
Vol 20 (2) ◽  
pp. 291-293
Author(s):  
Timothy A. R. Peacock ◽  
Leon W. Browder ◽  
Gilbert A. Schultz
Keyword(s):  
Lactate Dehydrogenase ◽  
Isozyme Pattern ◽  
Follicle Cells ◽  
Isozyme Patterns ◽  
Ldh Isozymes

It has recently been reported that the rabbit oocyte lactate dehydrogenase (LDH) isozyme pattern shifts dramatically at ovulation. We have determined that both ovarian oocytes and ovulated eggs have predominantly LDH-5 with minor amounts of LDH-4. Therefore, no change occurs at ovulation. When oocytes or eggs are not treated to remove follicle cells, all five LDH isozymes are observed. Isolated follicle cells also have five isozymes. We suggest that failure to remove follicle cells can cause altered LDH isozyme patterns and may account for conflicting results in the literature.

scholarly journals Urinary Lactate Dehydrogenase Activity and Its Isozyme Patterns in Kawasaki Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-6
Author(s):  
Yoichi Kawamura ◽  
Seiichiro Takeshita ◽  
Takashi Kanai ◽  
Mari Takizawa ◽  
Yusuke Yoshida ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Kawasaki Disease ◽  
Pattern Analysis ◽  
Upper Urinary Tract ◽  
Potential Significance ◽  
Lactate Dehydrogenase Activity ◽  
Isozyme Patterns ◽  
Ldh Activity ◽  
Sterile Pyuria ◽  
Ldh Isozymes

Abnormal urinary findings, such as sterile pyuria, proteinuria, and microscopic hematuria, are often seen in the acute phase of Kawasaki disease (KD). We investigated the potential significance of urinary lactate dehydrogenase (U-LDH) activity and its isozyme patterns in KD. Total U-LDH activity and its isozymes (U-LDH1-5) levels were compared among 120 patients with KD, 18 patients with viral infection (VI), and 43 patients with upper urinary tract infection (UTI) and additionally compared between intravenous immunoglobulin (IVIG) responders (n=89) and nonresponders (n=31) with KD. Total U-LDH activity was higher in KD (35.4±4.8 IU/L, P<0.05) and UTI patients (66.0±8.0 IU/L, P<0.01) than in VI patients (17.0±6.2 IU/L). In the isozyme pattern analysis, KD patients had high levels of U-LDH1 and U-LDH2, while UTI patients had high levels of U-LDH3, U-LDH4, and U-LDH5. Furthermore, IVIG nonresponders of KD had significantly higher levels of total U-LDH activity (45.1±4.7 IU/L, P<0.05), especially U-LDH1 and U-LDH2 (P<0.05), than IVIG responders (32.0±2.8 IU/L). KD patients have increased levels of total U-LDH activity, especially U-LDH-1 and U-LDH2, indicating a unique pattern of U-LDH isozymes different from that in UTI patients.

THE ACTIVITY OF LACTATE DEHYDROGENASE ISOZYMES DURING THYROXINE-INDUCED TADPOLE METAMORPHOSIS

1966 ◽  
Vol 44 (3) ◽  
pp. 303-310 ◽  
Author(s):  
H. C. Kim ◽  
A. D'Iorio ◽  
W. K. Paik
Keyword(s):  
Lactate Dehydrogenase ◽  
Isozyme Pattern ◽  
Improved Method ◽  
Lactate Dehydrogenase Isozymes ◽  
Ldh Activity ◽  
Thyroxine Treatment ◽  
Tadpole Tail ◽  
Ldh Isozymes

A new improved method to quantitate the amount of the isozymes of lactate dehydrogenase (LDH) has been devised. The method is sensitive and very reproducible. This method has been employed for studies on LDH isozymes during thyroxine-induced tadpole metamorphosis. Only one isozyme is present in tadpole liver whereas three isozymes are present in tadpole tail and brain. Thyroxine treatment produced a decrease of the total LDH activity of tadpole liver, tail, and brain; however, the relative amount of the isozyme F3 in tail and brain is the only fraction which shows a decrease. The F1 and F2 fractions show an increase in relative amount. Thalidomide has no influence on the isozyme pattern of LDH in thyroxine-induced metamorphosis of the tadpole.

Comparative Electrophoretic Patterns of Lactate Dehydrogenase in Three Species of Trout

1968 ◽  
Vol 25 (7) ◽  
pp. 1323-1331 ◽  
Author(s):  
G. R. Bouck ◽  
R. C. Ball
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Tissue Specificity ◽  
Polyacrylamide Gel ◽  
Isozyme Patterns ◽  
Electrophoretic Patterns ◽  
Species Specific ◽  
Ldh Isozymes

The isozyme patterns of lactate dehydrogenase (LDH) were examined in three species of trout using electrophoresis in polyacrylamide gel slabs. Each pattern was species specific and contained 15 isozymes of LDH, but a hybrid trout had at least 27 LDH isozymes. Tissue specificity of these isozymes was low except for skeletal muscle. These results suggest that LDH isozyme patterns could be used for taxonomic and diagnostic purposes.

scholarly journals THE DISTRIBUTION OF LACTATE DEHYDROGENASE ISOZYMES IN HUMAN SKELETAL MUSCLE FIBERS

1964 ◽  
Vol 12 (8) ◽  
pp. 608-614 ◽  
Author(s):  
M. VAN WIJHE ◽  
M. C. BLANCHAER ◽  
S. ST. GEORGE-STUBBS
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Human Skeletal Muscle ◽  
Muscle Fibers ◽  
Isozyme Pattern ◽  
Electrophoretic Separation ◽  
Lactate Dehydrogenase Isozymes ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme ◽  
Normal Human

A study of the distribution of lactate dehydrogenase isozymes in single fibers from normal human skeletal muscle is presented. The fibers were classified into red, intermediate and white types on histochemical grounds and their lactate dehydrogenase isozyme content assessed by electrophoretic separation in veronal buffered agar. The results generally agreed with previous homogenate studies on animal skeletal muscle, in that the white fibers contained almost exclusively isozymes IV and V, whereas red fibers were rich in isozymes I, II and III, but IV and V also appeared indigenous to these fibers. The intermediate fibers had an isozyme pattern combining the features of red and white fibers. The metabolic implications of these findings are discussed.

scholarly journals DEVELOPMENTAL CHANGE AND REGIONAL DIFFERENCE OF THE LACTATE DEHYDROGENASE ISOZYME PATTERN IN RAT BRAIN

1980 ◽  
Vol 13 (2) ◽  
pp. 164-172
Author(s):  
KANKATSU YUN ◽  
TAKESHI MATSUO ◽  
TAKASHI ORIBE ◽  
TSUTOMU TOMIOKA ◽  
TAKAYOSHI IKEDA
Keyword(s):  
Lactate Dehydrogenase ◽  
Rat Brain ◽  
Regional Difference ◽  
Developmental Change ◽  
Isozyme Pattern ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme

Differences in Isozyme Patterns of Nucleic and Cytoplasmic Lactate Dehydrogenase of Rat Liver Cells

Nature ◽  
1968 ◽  
Vol 217 (5125) ◽  
pp. 251-253 ◽  
Author(s):  
V. L. NEMCHINSKAYA ◽  
L. SH. GANELINA ◽  
A. D. BRAUN
Keyword(s):  
Lactate Dehydrogenase ◽  
Rat Liver ◽  
Liver Cells ◽  
Isozyme Patterns ◽  
Rat Liver Cells
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