Comparative Electrophoretic Patterns of Lactate Dehydrogenase in Three Species of Trout

1968 ◽  
Vol 25 (7) ◽  
pp. 1323-1331 ◽  
Author(s):  
G. R. Bouck ◽  
R. C. Ball
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Tissue Specificity ◽  
Polyacrylamide Gel ◽  
Isozyme Patterns ◽  
Electrophoretic Patterns ◽  
Species Specific ◽  
Ldh Isozymes

The isozyme patterns of lactate dehydrogenase (LDH) were examined in three species of trout using electrophoresis in polyacrylamide gel slabs. Each pattern was species specific and contained 15 isozymes of LDH, but a hybrid trout had at least 27 LDH isozymes. Tissue specificity of these isozymes was low except for skeletal muscle. These results suggest that LDH isozyme patterns could be used for taxonomic and diagnostic purposes.

scholarly journals ISOZYME HISTOCHEMISTRY: THE DISPLAY OF SELECTIVE LACTATE DEHYDROGENASE ISOZYMES IN SECTIONS OF SKELETAL MUSCLE

1964 ◽  
Vol 12 (9) ◽  
pp. 687-695 ◽  
Author(s):  
IRWIN A. BRODY ◽  
W. KING ENGEL
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Guinea Pig ◽  
Fast Moving ◽  
Type I ◽  
Type Ii ◽  
Electrophoretic Patterns ◽  
Slow Moving ◽  
Ldh Isozymes ◽  
Type Ii Fibers

The addition of chemical agents to the standard lactate dehydrogenase (LDH) incubating medium permits the histochemical display of selective LDH isozymes. Excess lactate decreases the intensity of the fast-moving isozymes, and urea decreases the intensity of the slow-moving isozymes. Application of these modified incubating media to histologic sections allows the display of selective LDH isozymes in unhomogenized tissues. Thus, predominance of the slow-moving LDH isozymes in guinea pig gastroenemius and of the fast-moving isozymes in guinea pig soleus were found on the electrophoretic patterns of the muscle extracts and confirmed on the intact tissues. The modified incubating media were used to localize specific LDH isozymes in normal human skeletal muscle. Ry extraction of the sections with saline or acetone the intracellular sites of isozyme activity were identified with recognized cell constituents. It was concluded that the slow-moving LDH isozymes predominate in the aqueous sarcoplasm of Type I fibers while the fast-moving isozymes predominate in the aqueous sarcoplasm of Type II fibers and in the mitochondria and lipid-mitochondrial complexes. The Pattern of dark and light fibers, seen after incubation in the standard LDH medium, was found to be due to the greater activity of the slow-moving isozymes in the Type I than the Type II fibers. Thus technique of isozyme histochemistry thus allows study of the intracellular localizations and the biochemical roles of specific LDH isozymes in skeletal muscles.

scholarly journals Urinary Lactate Dehydrogenase Activity and Its Isozyme Patterns in Kawasaki Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-6
Author(s):  
Yoichi Kawamura ◽  
Seiichiro Takeshita ◽  
Takashi Kanai ◽  
Mari Takizawa ◽  
Yusuke Yoshida ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Kawasaki Disease ◽  
Pattern Analysis ◽  
Upper Urinary Tract ◽  
Potential Significance ◽  
Lactate Dehydrogenase Activity ◽  
Isozyme Patterns ◽  
Ldh Activity ◽  
Sterile Pyuria ◽  
Ldh Isozymes

Abnormal urinary findings, such as sterile pyuria, proteinuria, and microscopic hematuria, are often seen in the acute phase of Kawasaki disease (KD). We investigated the potential significance of urinary lactate dehydrogenase (U-LDH) activity and its isozyme patterns in KD. Total U-LDH activity and its isozymes (U-LDH1-5) levels were compared among 120 patients with KD, 18 patients with viral infection (VI), and 43 patients with upper urinary tract infection (UTI) and additionally compared between intravenous immunoglobulin (IVIG) responders (n=89) and nonresponders (n=31) with KD. Total U-LDH activity was higher in KD (35.4±4.8 IU/L, P<0.05) and UTI patients (66.0±8.0 IU/L, P<0.01) than in VI patients (17.0±6.2 IU/L). In the isozyme pattern analysis, KD patients had high levels of U-LDH1 and U-LDH2, while UTI patients had high levels of U-LDH3, U-LDH4, and U-LDH5. Furthermore, IVIG nonresponders of KD had significantly higher levels of total U-LDH activity (45.1±4.7 IU/L, P<0.05), especially U-LDH1 and U-LDH2 (P<0.05), than IVIG responders (32.0±2.8 IU/L). KD patients have increased levels of total U-LDH activity, especially U-LDH-1 and U-LDH2, indicating a unique pattern of U-LDH isozymes different from that in UTI patients.

LACTATE DEHYDROGENASE IN RABBIT OOCYTES AND EGGS

1978 ◽  
Vol 20 (2) ◽  
pp. 291-293
Author(s):  
Timothy A. R. Peacock ◽  
Leon W. Browder ◽  
Gilbert A. Schultz
Keyword(s):  
Lactate Dehydrogenase ◽  
Isozyme Pattern ◽  
Follicle Cells ◽  
Isozyme Patterns ◽  
Ldh Isozymes

It has recently been reported that the rabbit oocyte lactate dehydrogenase (LDH) isozyme pattern shifts dramatically at ovulation. We have determined that both ovarian oocytes and ovulated eggs have predominantly LDH-5 with minor amounts of LDH-4. Therefore, no change occurs at ovulation. When oocytes or eggs are not treated to remove follicle cells, all five LDH isozymes are observed. Isolated follicle cells also have five isozymes. We suggest that failure to remove follicle cells can cause altered LDH isozyme patterns and may account for conflicting results in the literature.

scholarly journals Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle

2016 ◽  
Vol 291 (49) ◽  
pp. 25306-25318 ◽  
Author(s):  
Xijun Liang ◽  
Lin Liu ◽  
Tingting Fu ◽  
Qian Zhou ◽  
Danxia Zhou ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Mitochondrial Function

IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

scholarly journals THE DISTRIBUTION OF LACTATE DEHYDROGENASE ISOZYMES IN HUMAN SKELETAL MUSCLE FIBERS

1964 ◽  
Vol 12 (8) ◽  
pp. 608-614 ◽  
Author(s):  
M. VAN WIJHE ◽  
M. C. BLANCHAER ◽  
S. ST. GEORGE-STUBBS
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Human Skeletal Muscle ◽  
Muscle Fibers ◽  
Isozyme Pattern ◽  
Electrophoretic Separation ◽  
Lactate Dehydrogenase Isozymes ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme ◽  
Normal Human

A study of the distribution of lactate dehydrogenase isozymes in single fibers from normal human skeletal muscle is presented. The fibers were classified into red, intermediate and white types on histochemical grounds and their lactate dehydrogenase isozyme content assessed by electrophoretic separation in veronal buffered agar. The results generally agreed with previous homogenate studies on animal skeletal muscle, in that the white fibers contained almost exclusively isozymes IV and V, whereas red fibers were rich in isozymes I, II and III, but IV and V also appeared indigenous to these fibers. The intermediate fibers had an isozyme pattern combining the features of red and white fibers. The metabolic implications of these findings are discussed.

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