THE ACTIVITY OF LACTATE DEHYDROGENASE ISOZYMES DURING THYROXINE-INDUCED TADPOLE METAMORPHOSIS

1966 ◽  
Vol 44 (3) ◽  
pp. 303-310 ◽  
Author(s):  
H. C. Kim ◽  
A. D'Iorio ◽  
W. K. Paik
Keyword(s):  
Lactate Dehydrogenase ◽  
Isozyme Pattern ◽  
Improved Method ◽  
Lactate Dehydrogenase Isozymes ◽  
Ldh Activity ◽  
Thyroxine Treatment ◽  
Tadpole Tail ◽  
Ldh Isozymes

A new improved method to quantitate the amount of the isozymes of lactate dehydrogenase (LDH) has been devised. The method is sensitive and very reproducible. This method has been employed for studies on LDH isozymes during thyroxine-induced tadpole metamorphosis. Only one isozyme is present in tadpole liver whereas three isozymes are present in tadpole tail and brain. Thyroxine treatment produced a decrease of the total LDH activity of tadpole liver, tail, and brain; however, the relative amount of the isozyme F3 in tail and brain is the only fraction which shows a decrease. The F1 and F2 fractions show an increase in relative amount. Thalidomide has no influence on the isozyme pattern of LDH in thyroxine-induced metamorphosis of the tadpole.

scholarly journals THE DISTRIBUTION OF LACTATE DEHYDROGENASE ISOZYMES IN HUMAN SKELETAL MUSCLE FIBERS

1964 ◽  
Vol 12 (8) ◽  
pp. 608-614 ◽  
Author(s):  
M. VAN WIJHE ◽  
M. C. BLANCHAER ◽  
S. ST. GEORGE-STUBBS
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Human Skeletal Muscle ◽  
Muscle Fibers ◽  
Isozyme Pattern ◽  
Electrophoretic Separation ◽  
Lactate Dehydrogenase Isozymes ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme ◽  
Normal Human

A study of the distribution of lactate dehydrogenase isozymes in single fibers from normal human skeletal muscle is presented. The fibers were classified into red, intermediate and white types on histochemical grounds and their lactate dehydrogenase isozyme content assessed by electrophoretic separation in veronal buffered agar. The results generally agreed with previous homogenate studies on animal skeletal muscle, in that the white fibers contained almost exclusively isozymes IV and V, whereas red fibers were rich in isozymes I, II and III, but IV and V also appeared indigenous to these fibers. The intermediate fibers had an isozyme pattern combining the features of red and white fibers. The metabolic implications of these findings are discussed.

scholarly journals Urinary Lactate Dehydrogenase Activity and Its Isozyme Patterns in Kawasaki Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-6
Author(s):  
Yoichi Kawamura ◽  
Seiichiro Takeshita ◽  
Takashi Kanai ◽  
Mari Takizawa ◽  
Yusuke Yoshida ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Kawasaki Disease ◽  
Pattern Analysis ◽  
Upper Urinary Tract ◽  
Potential Significance ◽  
Lactate Dehydrogenase Activity ◽  
Isozyme Patterns ◽  
Ldh Activity ◽  
Sterile Pyuria ◽  
Ldh Isozymes

Abnormal urinary findings, such as sterile pyuria, proteinuria, and microscopic hematuria, are often seen in the acute phase of Kawasaki disease (KD). We investigated the potential significance of urinary lactate dehydrogenase (U-LDH) activity and its isozyme patterns in KD. Total U-LDH activity and its isozymes (U-LDH1-5) levels were compared among 120 patients with KD, 18 patients with viral infection (VI), and 43 patients with upper urinary tract infection (UTI) and additionally compared between intravenous immunoglobulin (IVIG) responders (n=89) and nonresponders (n=31) with KD. Total U-LDH activity was higher in KD (35.4±4.8 IU/L, P<0.05) and UTI patients (66.0±8.0 IU/L, P<0.01) than in VI patients (17.0±6.2 IU/L). In the isozyme pattern analysis, KD patients had high levels of U-LDH1 and U-LDH2, while UTI patients had high levels of U-LDH3, U-LDH4, and U-LDH5. Furthermore, IVIG nonresponders of KD had significantly higher levels of total U-LDH activity (45.1±4.7 IU/L, P<0.05), especially U-LDH1 and U-LDH2 (P<0.05), than IVIG responders (32.0±2.8 IU/L). KD patients have increased levels of total U-LDH activity, especially U-LDH-1 and U-LDH2, indicating a unique pattern of U-LDH isozymes different from that in UTI patients.

Genetics of Multiple Lactate Dehydrogenase Isozymes in Muscle Tissue of Lake Whitefish (Coregonus clupeaformis)

1970 ◽  
Vol 27 (6) ◽  
pp. 1115-1121 ◽  
Author(s):  
J. W. Clayton ◽  
W. G. Franzin
Keyword(s):  
Lactate Dehydrogenase ◽  
Lateral Line ◽  
White Muscle ◽  
Heart Tissue ◽  
Coregonus Clupeaformis ◽  
Lake Whitefish ◽  
Lactate Dehydrogenase Isozymes ◽  
Tissue Extracts ◽  
Fish Breeding ◽  
Ldh Isozymes

Electrophoresis of extracts of red lateral line muscle from lake whitefish (Coregonus clupeaformis) revealed two groups of lactate dehydrogenase (LDH) isozymes. In heart tissue extracts the faster moving set of isozymes predominated whereas most of the LDH in white muscle consisted of the slower set of electrophoretic isozymes.Three phenotypes of heart-type LDH isozymes were found in wild fish. Breeding experiments showed that there were two nondominant alleles at the locus coding the most anodal of the heart-type LDH subunits. The frequencies of these alleles were different in fish from two lakes.Our observations regarding the presence of discrete sets of multiple LDH isozymes in lake whitefish and the independent genetic control of isozymes within each set provide additional evidence for the probable tetraploid nature of salmonids.

scholarly journals A case of an lactate dehydrogenase (LDH)-IgG complex formation which causes low plasma LDH activity and an abnormal LDH isozyme pattern.

1986 ◽  
Vol 25 (2) ◽  
pp. 175-178
Author(s):  
Yoshihiro MORI ◽  
Kazuaki SHIMAMOTO ◽  
Mitsuhiro NISHIMURA ◽  
Teiichi SASAKI ◽  
Takashi KANNO ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Complex Formation ◽  
Isozyme Pattern ◽  
Ldh Activity

Variants of Lactate Dehydrogenase Isozymes in Sera of Sockeye Salmon (Oncorhynchus nerka)

1969 ◽  
Vol 26 (1) ◽  
pp. 15-19 ◽  
Author(s):  
H. O. Hodgins ◽  
W. E. Ames ◽  
F. M. Utter
Keyword(s):  
British Columbia ◽  
Lactate Dehydrogenase ◽  
Sockeye Salmon ◽  
Oncorhynchus Nerka ◽  
Gulf Of Alaska ◽  
Bristol Bay ◽  
Lactate Dehydrogenase Isozymes ◽  
Skeena River ◽  
Total Body ◽  
Ldh Isozymes

Three phenotypes of lactate dehydrogenase (LDH) isozymes were found in sera of sockeye salmon (Oncorhynchus nerka), presumably representing B′B′, B′B, and BB genotypes. No association was obvious between LDH phenotype of sera and sex or total body length.Of 1006 sera from Asian, Bristol Bay, and Gulf of Alaska stocks, 826 were B′B′ and 180 were B′B or BB. Of 591 sera from Washington and British Columbia stocks, 589 were B′B′ and 2 were B′B; both of the B-allele phenotypes were found in fish captured at the Skeena River in northern British Columbia. These findings suggested that LDH isozymes should be useful in studies on ocean distribution of sockeye salmon and in characterizing certain Asian and Alaskan sockeye salmon populations.

LACTATE DEHYDROGENASE IN RABBIT OOCYTES AND EGGS

1978 ◽  
Vol 20 (2) ◽  
pp. 291-293
Author(s):  
Timothy A. R. Peacock ◽  
Leon W. Browder ◽  
Gilbert A. Schultz
Keyword(s):  
Lactate Dehydrogenase ◽  
Isozyme Pattern ◽  
Follicle Cells ◽  
Isozyme Patterns ◽  
Ldh Isozymes

It has recently been reported that the rabbit oocyte lactate dehydrogenase (LDH) isozyme pattern shifts dramatically at ovulation. We have determined that both ovarian oocytes and ovulated eggs have predominantly LDH-5 with minor amounts of LDH-4. Therefore, no change occurs at ovulation. When oocytes or eggs are not treated to remove follicle cells, all five LDH isozymes are observed. Isolated follicle cells also have five isozymes. We suggest that failure to remove follicle cells can cause altered LDH isozyme patterns and may account for conflicting results in the literature.

scholarly journals Structural adaptations of lactate dehydrogenase isozymes.

1977 ◽  
Vol 74 (7) ◽  
pp. 2677-2681 ◽  
Author(s):  
W. Eventoff ◽  
M. G. Rossmann ◽  
S. S. Taylor ◽  
H. J. Torff ◽  
H. Meyer ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Lactate Dehydrogenase Isozymes ◽  
Structural Adaptations
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