GIEMSA BANDING IN LOLIUM TEMULENTUM

1977 ◽  
Vol 19 (4) ◽  
pp. 663-666 ◽  
Author(s):  
H. M. Thomas
Keyword(s):  
Secondary Constriction ◽  
Staining Technique ◽  
Giemsa Staining ◽  
Giemsa Banding ◽  
Banding Patterns ◽  
Lolium Temulentum

A Giemsa staining technique has been applied to the somatic chromosomes of the inbreeding diploid grass Lolium temulentum (2n = 14). Bands appear in six of the seven pairs and are associated either with the centromere or secondary constriction. An idiogram of the chromosomes with their banding patterns is presented.

G and/or C-bands in plant chromosomes?

1984 ◽  
Vol 71 (1) ◽  
pp. 111-120
Author(s):  
I. Schubert ◽  
R. Rieger ◽  
P. Dobel
Keyword(s):  
Constitutive Heterochromatin ◽  
Giemsa Staining ◽  
Giemsa Banding ◽  
Base Pairs ◽  
Banding Patterns ◽  
C Banding ◽  
Plant Chromosomes ◽  
Nucleolus Organizing Region ◽  
Similarities And Differences ◽  
Simple Sequence

Similarities and differences become evident from comparisons of centromeric and non-centromeric banding patterns in plant and animal chromosomes. Similar to C and G-banding in animals (at least most of the reptiles, birds and mammals), centromeric and nucleolus-organizing region bands as well as interstitially and/or terminally located non-centromeric bands may occur in plants, depending on the kind and strength of pretreatment procedures. The last group of bands may sometimes be subdivided into broad regularly occurring ‘marker’ bands and thinner bands of more variable appearance. Non-centromeric bands in plants often correspond to blocks of constitutive heterochromatin that are rich in simple sequence DNA and sometimes show polymorphism; they thus resemble C-bands. However, most of these bands contain late-replicating DNA. Also they are sometimes rich A X T base-pairs, closely adjacent to each other and positionally identical to Feulgen+ and Q+ bands, thus being comparable to mammalian G-bands. Although banding that is reverse to the non-centromeric bands after Giemsa staining is still uncertain in plants, reverse banding patterns can be obtained with Feulgen or with pairs of A X T versus G X C-specific fluorochromes. It is therefore concluded that not all of the plant Giemsa banding patterns correspond to C-banding of mammalian chromosomes. Before the degree of homology between different Giemsa banding patterns in plants and G and/or C-bands in mammals is finally elucidated, the use of the neutral term ‘Giemsa band’, specified by position (e.g. centromeric, proximal, interstitial, terminal), is suggested to avoid confusion.

DIFFERENTIAL GIEMSA STAINING IN PLANTS. V. TWO TYPES OF CONSTITUTIVE HETEROCHROMATIN IN SPECIES OF AVENA

1977 ◽  
Vol 19 (4) ◽  
pp. 739-743 ◽  
Author(s):  
Sheng-Tian Yen ◽  
W. Gary Filion
Keyword(s):  
Room Temperature ◽  
Chromosome Banding ◽  
Constitutive Heterochromatin ◽  
Diploid Species ◽  
The Other ◽  
Giemsa Staining ◽  
Barium Hydroxide ◽  
Giemsa Banding ◽  
Banding Patterns ◽  
Hydrolysis Temperature

Modified ASG (Acetic/Saline/Giemsa) and BSG (Barium hydroxide/Saline/Giemsa) chromosome banding techniques applied to several diploid species of oats produced two distinct types of C-banding patterns. One pattern consisted mainly of centromeric bands with occasional telomeric and/or intercalary bands while the other was comprised only of prominent telomeric and intercalary bands. These two banding patterns which probably reflect two distinct types of constitutive heterochromatin resulted from a change in the HCl hydrolysis temperature prior to the application of the ASG or BSG technique; hydrolysis at 60 °C yielded the centromeric bands and hydrolysis at room temperature produced telomeric and intercalary bands. Since all species examined reacted in a similar manner, precise Giemsa banding patterns should now be possible for all or most species of oats.

Giemsa banding patterns in the chromosomes of twelve species of cats (Felidae)

1973 ◽  
Vol 12 (6) ◽  
pp. 377-397 ◽  
Author(s):  
Doris H. Wurster-Hill ◽  
C.W. Gray
Keyword(s):  
Giemsa Banding ◽  
Banding Patterns

Identical Giemsa banding patterns of two Macaca species: Macaca mulatta and M. fascicularis

1975 ◽  
Vol 14 (1) ◽  
pp. 26-33 ◽  
Author(s):  
G.F. de Vries ◽  
H.F. de France ◽  
J.A.M. Schevers
Keyword(s):  
Macaca Mulatta ◽  
Giemsa Banding ◽  
Banding Patterns

Comparison of chromosome BrdU-Hoechst-Giemsa banding patterns of the A1 and (AD)2 genomes of cotton

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 616-625 ◽  
Author(s):  
Olga V. Muravenko ◽  
Alexander R. Fedotov ◽  
Elizabeth O. Punina ◽  
Ludmila I. Fedorova ◽  
Valerii G. Grif ◽  
...  
Keyword(s):  
Giemsa Banding ◽  
Banding Patterns

A study of heterochromatin in Drosophila nasuta by the 5-bromodeoxyuridine-giemsa staining technique

Chromosoma ◽  
1979 ◽  
Vol 72 (2) ◽  
pp. 249-255 ◽  
Author(s):  
S. C. Lakhotia ◽  
J. K. Roy ◽  
Mahesh Kumar
Keyword(s):  
Staining Technique ◽  
Giemsa Staining ◽  
Drosophila Nasuta

Analysis of high-resolution R-bands, obtained by heat-denaturation and Giemsa staining, on human prophase chromosomes

1985 ◽  
Vol 27 (1) ◽  
pp. 83-91 ◽  
Author(s):  
R. Drouin ◽  
C. L. Richer
Keyword(s):  
High Resolution ◽  
Banding Pattern ◽  
Giemsa Staining ◽  
Heat Denaturation ◽  
Banding Patterns ◽  
International Standard

RHG-bands (heat-denatured Giemsa R-bands) of human prophase chromosomes were analyzed at high resolution, and the banding patterns at prophase and metaphase are presented. The bands were compared with those of the International Standard Cytogenetic Nomenclature idiograms and of the G-band idiograms proposed by J. J. Yunis. The number, size, and position of the RHG-bands correspond rather well with their equivalent G-negative bands, but some differences were noted in the zones of preferential stretching, the juxtacentromeric regions, and the telomeres. Variations in the centromere index and the banding pattern in heterochromatin were also discussed.Key words: human prophase chromosomes, RHG-bands, high-resolution chromosomes.

Comparison of chromosome BrdU-Hoechst-Giemsa banding patterns of the A1 and (AD)2 genomes of cotton

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 616-625 ◽  
Author(s):  
Olga V Muravenko ◽  
Alexander R Fedotov ◽  
Elizabeth O Punina ◽  
Ludmila I Fedorova ◽  
Valerii G Grif ◽  
...  
Keyword(s):  
Image Analysis ◽  
Complete Classification ◽  
Small Chromosome ◽  
Tetraploid Cotton ◽  
Image Analysis System ◽  
Giemsa Banding ◽  
Genome Chromosome ◽  
Banding Patterns ◽  
S Period ◽  
Analysis System

The karyotypes of diploid cotton, Gossypium herbaceum L. var. africanum (Watt) Mauer, and tetraploid cotton, Gossypium barbadense L., were studied by BrdU-Hoechst-Giemsa banding, using a specially developed image-analysis system. The patterns obtained are represented by the slightly and intensively stained bands that correspond, respectively, to the early replicating DNA and the DNA replicating in the mid and late S period. The number of main Giemsa-positive bands varies from 2 to 9 per chromosome. The banding patterns of all homologous pairs are specific in both the A1 and (AD)2 genomes. This made possible the complete classification of the chromosomes. Based on the similarity of the BrdU-Hoechst-Giemsa banding patterns and the sizes of the chromosomes in the A1 and (AD)2 genomes, we divided the (AD)2 genome into Ab and Db subgenomes and classified their chromosomes according to the A1 genome chromosome classification. The BrdU-Hoechst-Giemsa banding pattern of the Db subgenome is basically similar to that of the A1 genome and Ab subgenome, but the differences between it and the banding patterns of the A1 genome and Ab subgenome are more significant than the differences between the latter two genomes. The similarity of the intragenomic banding patterns between nonhomologous chromosomes a and b, c and g, d and e, f and j, h and i, and l and m was revealed. Based on our results, we suggest that the ancestral cotton genome contained 7 homologous pairs of chromosomes. The results prove the feasibility of image-analysis techniques for identification and quantitative analysis of chromosomes, especially with regard to small-chromosome species.Key words: cotton, A1 and (AD)2 genomes, chromosome identification, BrdU-Hoechst-Giemsa banding, image analysis.

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