ACTIVITY AND RELATIVE MOBILITY OF PEROXIDASE AND ESTERASE ISOZYMES OF FLAX (LINUM USITATISSIMUM) GENOTROPHS. I. DEVELOPING MAIN STEMS

1973 ◽  
Vol 15 (4) ◽  
pp. 731-744 ◽  
Author(s):  
M. A. Fieldes ◽  
H. Tyson
Keyword(s):  
Linum Usitatissimum ◽  
Relative Mobility ◽  
Esterase Isozymes ◽  
Anionic Peroxidase ◽  
Rates Of Change ◽  
Peroxidase Isozymes

The effects of growth of one flax genotype in soil supplemented by either nitrogen, phosphorous and potassium (NPK) or nitrogen and potassium (NK) on its progeny produced by several generations of complete selfing were studied. The two types of progeny produced, L induced by NPK and S induced by NK, were examined at 4-day intervals from the 16th to the 36th day after sowing. Both activity and relative mobility of the anionic peroxidase and esterase isozymes displayed effects of the fertilizer treatments applied in the treatment generation. NPK, compared to NK, increased the relative mobility of all the peroxidase and esterase isozymes, while depressing the activities of the peroxidase isozymes and increasing those of the esterase isozymes, at all ages examined. Some differences in the rates of change, with age, of the characteristics of L and S were distinguished; the differences observed between L and S could not, however, be attributed to differences in their developmental ages.

DIFFERENCES IN MOLECULAR WEIGHT BETWEEN CORRESPONDING ANIONIC PEROXIDASE ISOZYMES FROM TWO FLAX GENOTROPHS

1980 ◽  
Vol 22 (4) ◽  
pp. 529-534 ◽  
Author(s):  
H. Tyson ◽  
M. A. Fieldes
Keyword(s):  
Molecular Weight ◽  
Linear Regression ◽  
Linum Usitatissimum ◽  
Gel Electrophoresis ◽  
Relative Mobility ◽  
Anionic Peroxidase ◽  
Linum Usitatissimum L ◽  
Peroxidase Isozymes ◽  
Flax Genotrophs ◽  
Adult Plants

Anionic peroxidase isozymes from main stem tissues of adult plants of two flax (Linum usitatissimum L.) genotrophs were separated using acrylamide gel electrophoresis. A range of seven acrylamide concentrations was used for the gels, enabling the effect of gel concentration on relative mobility (Rm) to be examined. The regression of log (Rm) on gel concentration was linear for two of the four main isozymes found. Differences in linear regression slope between the L and S flax genotroph isozymes suggested genotroph differences in molecular weight.

ACTIVITY AND RELATIVE MOBILITY OF PEROXIDASE ISOENZYMES IN GENOTROPHS AND GENOTYPES OF FLAX (LINUM USITATISSIMUM L.)

1972 ◽  
Vol 14 (3) ◽  
pp. 625-636 ◽  
Author(s):  
M. A. Fieldes ◽  
H. Tyson
Keyword(s):  
Linum Usitatissimum ◽  
Gel Electrophoresis ◽  
Relative Mobility ◽  
Peroxidase Isoenzymes ◽  
Anionic Peroxidase ◽  
Linum Usitatissimum L ◽  
Phosphorus And Potassium ◽  
Highly Correlated ◽  
Successive Generations ◽  
Nitrogen Phosphorus

Acrylamide gel electrophoresis was used to separate anionic peroxidase isoenzymes in genotypes and genotrophs of flax. Activities and relative mobilities were measured directly from the separations on the gels.The effects of growth of one flax genotype in soil supplemented by either nitrogen, phosphorus and potassium (NPK) or nitrogen and potassium (NK) on subsequent generations of its progeny produced by complete selfing were studied. Both activity and relative mobility of anionic peroxidase isoenzymes displayed effects of fertilizer treatments applied in previous generations. NPK increased the relative mobility of all isoenzymes, while depressing the activity of at least three of them. Successive generations of growth in NPK produced approximately linear increases in relative mobility. Such environmentally induced heritable changes were detectable five generations later.Two other flax genotypes were crossed, and relative mobility and activity of anionic peroxidase isoenzymes were examined in both parents and F2 progeny. Between parents, there were differences in relative mobility for two of the four isoenzymes; their F2 hybrids showed intermediate mobility for these particular isoenzymes. There were no differences between reciprocal F2 hybrids for mobility or activity of any isoenzyme. The parents differed in activity in all four isoenzymes; the F2 hybrids displayed dominance towards the lower activity parent for each of the isoenzymes.Total anionic isoenzyme activity was highly correlated with gross peroxidase activity measured prior to electrophoretic separation.

PROTEIN PROFILES OF FLAX GENOTROPHS

1982 ◽  
Vol 24 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Mary Ann Fieldes ◽  
Hugh Tyson ◽  
David Marriott
Keyword(s):  
Linum Usitatissimum ◽  
Plant Proteins ◽  
Two Dimensional ◽  
Stem Tissue ◽  
Protein Profiles ◽  
Relative Mobility ◽  
Two Dimensional Electrophoresis ◽  
Linum Usitatissimum L ◽  
Flax Genotrophs ◽  
Dimensional Electrophoresis

Protein profiles of partially purified protein extracts from main stem tissue of Durrant's L and S flax (Linum usitatissimum L.) genotrophs were examined with one and two dimensional electrophoresis on acrylamide gels. The purification retained mainly glycoproteins. Among this reduced spectrum of plant proteins, some of the proteins separated had relative mobility (Rm) shifts between L and S. For two proteins, the Rm shifts were demonstrated in two-dimensional separations using mixtures of the L and S extracts. The Rm shifts were all in the same direction, the S protein ran slightly slower than the corresponding L protein, in both dimensions. This shift direction agreed with previous studies on Rm shifts with peroxidase and esterase isozymes and with similar shifts in acid phosphatase isozymes.

Involvement of a novel peroxidase isozyme and lignification in hydathodes in resistance to black rot disease in cabbage

2000 ◽  
Vol 78 (9) ◽  
pp. 1144-1149 ◽  
Author(s):  
P A Gay ◽  
S Tuzun
Keyword(s):  
Xanthomonas Campestris ◽  
Virulent Strain ◽  
Black Rot ◽  
Peroxidase Isozyme ◽  
Anionic Peroxidase ◽  
Peroxidase Isozymes ◽  
Resistant Varieties ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris ◽  
Lignin Deposition

The physiological mechanisms associated with resistance of cabbage to black rot disease seem to be associated with the hydathodes. To investigate the role of hydathodes in disease resistance, total peroxidase activities, anionic peroxidase isozyme expression, and lignin deposition were determined in hydathodes of resistant (Hancock and Green Cup), partially resistant (Cheers), and susceptible (Strukton and Perfect Ball) cabbage varieties (Brassica oleracea L.) during pathogenesis with Xanthomonas campestris pv. campestris. Four-week-old plants were petiole-inoculated with a highly virulent strain of X. campestris pv. campestris (FD91L). Hydathodal fluids were collected daily over a 14-day period from infected plants as well as noninfected, mock-inoculated control plants. Hydathodal fluids of resistant varieties had greater peroxidase activity when compared to susceptible ones, with infected plants having higher peroxidase levels than noninfected plants. Isoelectric focusing revealed the presence of four anionic peroxidase isozymes in hydathodal fluids, with the most anionic one (pI of 3.6) accumulating only upon infection. Lignin deposition in and around the hydathodes was associated with the accumulation of this particular isozyme in hydathodal fluids. The evidence suggests that a rapid, systemic response is associated with resistance to the bacterial pathogen.Key words: peroxidases, hydathodes, isozymes, black rot disease, cabbage, Xanthomonas campestris pv. campestris.

Peroxidase activity and relative mobility at anthesis in flax genotrophs and their F2 progeny: developmental and genetic effects

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 495-504 ◽  
Author(s):  
M. A. Fieldes ◽  
J. Ross
Keyword(s):  
Genetic Control ◽  
Peroxidase Activity ◽  
Stem Elongation ◽  
Genetic Regulation ◽  
Relative Mobility ◽  
Activity Data ◽  
Peroxidase Isozymes ◽  
Developmental Age ◽  
Total Peroxidase Activity ◽  
Flax Genotrophs

The genetic regulation of the environmentally induced heritable difference in peroxidase activity between Durrant's large (L) and small (S) flax genotrophs was examined in leaves from plants ranging in developmental age from 6 days before anthesis to 3 days after. Mean peroxidase activity was higher for S than L and intermediate for the reciprocal F2's from L × S and S × L crosses (F2L × S and F2S × L). However, activity increased with development and, since there were small but significant differences in the average developmental ages of L, S, F2L × S, and F2S × L plants, the effects of development on activity had to be taken into account in examining the F2 activity data for segregation. A regression method was used to remove developmental effects and, underlying these effects, total peroxidase activity appeared to be regulated by a single locus with two alleles and L dominance. Two other dimorphic loci, both described previously, were also examined. One regulates the presence-absence of septa hairs in the seed capsules and the other the relative mobility of anionic peroxidase isozymes. There was no phenotypic linkage between the three segregating parameters. The genetic control of activity appeared to regulate cationic rather than anionic activity. In addition, a relationship between activity and plant height indicated either that peroxidase activity is one of the factors regulating main stem elongation or that the locus regulating peroxidase activity is linked to one of the loci involved in the regulation of plant height.Key words: flax genotrophs, peroxidase, genetic control, development.

scholarly journals Segregation of the environmentally induced relative mobility shifts in flax genotroph peroxidase isozymes

Heredity ◽  
1978 ◽  
Vol 40 (2) ◽  
pp. 281-290 ◽  
Author(s):  
H Tyson ◽  
S A Taylor ◽  
M A Fieldes
Keyword(s):  
Relative Mobility ◽  
Peroxidase Isozymes

ACTIVITY AND RELATIVE MOBILITY OF PEROXIDASE AND ESTERASE ISOZYMES OF FLAX (LINUM USITATISSIMUM) GENOTROPHS. II. F1 HYBRIDS AND NUCLEAR DNA REVERSION TYPES

1973 ◽  
Vol 15 (4) ◽  
pp. 745-755 ◽  
Author(s):  
M. A. Fieldes ◽  
H. Tyson
Keyword(s):  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
F1 Hybrids ◽  
Fresh Weight ◽  
Plant Weight ◽  
Esterase Isozymes ◽  
Anionic Peroxidase ◽  
Reciprocal Hybrids ◽  
Reciprocal Differences ◽  
The Mean

The effects of growth of one genotype of flax in soil supplemented by either nitrogen, phosphorous and potassium (NPK) or nitrogen and potassium (NK) on its progeny produced by several generations of complete selfing were studied. The two types of progeny produced, genotroph L, induced by NPK and genotroph S induced by NK, and their F1 reciprocal hybrids were examined. L and S differed in plant weight and in the activities and relative mobilities of their corresponding anionic peroxidase and esterase isozymes. No reciprocal differences were detected and the mean F1 values for all characteristics were intermediate between the parents, with the exception of the peroxidase isozyme relative mobilities which displayed dominance of parent L. The genotrophs were known to exhibit a 16% difference in nuclear DNA content which could be reverted to 0%, without altering the genotroph fresh weight difference, by growing the genotrophs in lower than normal temperatures. Examination of the peroxidase and esterase isozymes of nDNA reverted genotrophs showed that, with one possible exception, there was no accompanying reversion of the activities and relative mobilities of the peroxidase or esterase isozymes.

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