PROTEIN PROFILES OF FLAX GENOTROPHS

1982 ◽  
Vol 24 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Mary Ann Fieldes ◽  
Hugh Tyson ◽  
David Marriott
Keyword(s):  
Linum Usitatissimum ◽  
Plant Proteins ◽  
Two Dimensional ◽  
Stem Tissue ◽  
Protein Profiles ◽  
Relative Mobility ◽  
Two Dimensional Electrophoresis ◽  
Linum Usitatissimum L ◽  
Flax Genotrophs ◽  
Dimensional Electrophoresis

Protein profiles of partially purified protein extracts from main stem tissue of Durrant's L and S flax (Linum usitatissimum L.) genotrophs were examined with one and two dimensional electrophoresis on acrylamide gels. The purification retained mainly glycoproteins. Among this reduced spectrum of plant proteins, some of the proteins separated had relative mobility (Rm) shifts between L and S. For two proteins, the Rm shifts were demonstrated in two-dimensional separations using mixtures of the L and S extracts. The Rm shifts were all in the same direction, the S protein ran slightly slower than the corresponding L protein, in both dimensions. This shift direction agreed with previous studies on Rm shifts with peroxidase and esterase isozymes and with similar shifts in acid phosphatase isozymes.

DIFFERENCES IN MOLECULAR WEIGHT BETWEEN CORRESPONDING ANIONIC PEROXIDASE ISOZYMES FROM TWO FLAX GENOTROPHS

1980 ◽  
Vol 22 (4) ◽  
pp. 529-534 ◽  
Author(s):  
H. Tyson ◽  
M. A. Fieldes
Keyword(s):  
Molecular Weight ◽  
Linear Regression ◽  
Linum Usitatissimum ◽  
Gel Electrophoresis ◽  
Relative Mobility ◽  
Anionic Peroxidase ◽  
Linum Usitatissimum L ◽  
Peroxidase Isozymes ◽  
Flax Genotrophs ◽  
Adult Plants

Anionic peroxidase isozymes from main stem tissues of adult plants of two flax (Linum usitatissimum L.) genotrophs were separated using acrylamide gel electrophoresis. A range of seven acrylamide concentrations was used for the gels, enabling the effect of gel concentration on relative mobility (Rm) to be examined. The regression of log (Rm) on gel concentration was linear for two of the four main isozymes found. Differences in linear regression slope between the L and S flax genotroph isozymes suggested genotroph differences in molecular weight.

Two-dimensional electrophoresis of plant proteins and standardization of gel patterns

1996 ◽  
Vol 17 (5) ◽  
pp. 855-865 ◽  
Author(s):  
Akira Tsugita ◽  
Masaharu Kamo ◽  
Takao Kawakami ◽  
Yuta Ohki
Keyword(s):  
Plant Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativaL.) Proteomics

2012 ◽  
Vol 18 (5) ◽  
pp. 819 ◽  
Author(s):  
Yanhua YANG ◽  
Weitong CUI ◽  
Xiaoyong LIU ◽  
Keming ZHU ◽  
Keping CHEN
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
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