Spatial relations of human chromosomes identified by quinacrine fluorescence at metaphase

1973 ◽  
Vol 18 (4) ◽  
pp. 297-306 ◽  
Author(s):  
D. Warburton ◽  
A. F. Naylor ◽  
F. E. Warburton
Keyword(s):  
Spatial Relations ◽  
Human Chromosomes ◽  
Quinacrine Fluorescence

Spatial relations of human chromosomes identified by quinacrine fluorescence at metaphase

1973 ◽  
Vol 18 (4) ◽  
pp. 307-313 ◽  
Author(s):  
A. F. Naylor ◽  
D. Warburton ◽  
F. E. Warburton
Keyword(s):  
Spatial Relations ◽  
Human Chromosomes ◽  
Quinacrine Fluorescence

QUINACRINE FLUORESCENCE AND Q-BANDING PATTERNS OF HUMAN CHROMOSOMES.: I. Effects of Varying Factors

1975 ◽  
Vol 17 (1) ◽  
pp. 81-92 ◽  
Author(s):  
C. C. Lin ◽  
H. van de Sande ◽  
W. K. Smink ◽  
D. R. Newton
Keyword(s):  
Exposure Time ◽  
Uv Light ◽  
Human Chromosomes ◽  
Concave Mirror ◽  
Banding Patterns ◽  
Fluorescent Microscope ◽  
Sperm Dna ◽  
Quinacrine Fluorescence ◽  
Well Differentiated ◽  
Salmon Sperm Dna

Various factors involved in the production of "Q-bands" have been studied. It was found that a Zeiss standard WL fluorescent microscope required a shorter exposure time for photography as compared to a Zeiss photomicroscope. The minimal exposure time was obtained when the standard WL microscope was equipped with a UV light source containing a DC powered mercury burner and a concave mirror. Further, the pH and type of water used in the staining, washing and mounting of the slide were also important factors in producing clear and well differentiated "Q-bands". It also appears that the factors involved in the production of "Q-bands" effect the enhancement or quenching of fluorescence by poly d(A-T).poly d(A-T) and salmon sperm DNA or poly dG∙poly dC respectively. This preliminary report also suggests that DNA or polynucleotides with a specific base sequence may play an important role in Q-banding patterns on chromosomes.

GIEMSA STAINING OF HETEROCHROMATIC AREAS OF HUMAN CHROMOSOMES FOR IDENTIFICATION

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner
Keyword(s):  
Acetic Acid ◽  
Banding Pattern ◽  
Saline Solution ◽  
Simple Technique ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Dna Denaturation ◽  
Quinacrine Fluorescence ◽  
Inexpensive Procedure ◽  
Distinct Banding Pattern

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.

Improved preservation of the otolithic membranes

1987 ◽  
Vol 45 ◽  
pp. 914-915
Author(s):  
G. M. Cohen ◽  
J. S. Grasso ◽  
M. L. Domeier ◽  
P. T. Mangonon
Keyword(s):  
Toluidine Blue ◽  
Potassium Phosphate ◽  
Vestibular Function ◽  
Spatial Relations ◽  
White Leghorn ◽  
Supporting Cells ◽  
Semithin Sections ◽  
Micromechanical Models ◽  
Soluble Components

Any explanation of vestibular micromechanics must include the roles of the otolithic and cupular membranes. However, micromechanical models of vestibular function have been hampered by unresolved questions about the microarchitectures of these membranes and their connections to stereocilia and supporting cells. Otolithic membranes are notoriously difficult to preserve because of severe shrinkage and loss of soluble components. We have empirically developed fixation procedures that reduce shrinkage artifacts and more accurately depict the spatial relations between the otolithic membranes and the ciliary bundles and supporting cells.We used White Leghorn chicks, ranging in age from newly hatched to one week. The inner ears were fixed for 3-24 h in 1.5-1.75% glutaraldehyde in 150 mM KCl, buffered with potassium phosphate, pH 7.3; when postfixed, it was for 30 min in 1% OsO4 alone or mixed with 1% K4Fe(CN)6. The otolithic organs (saccule, utricle, lagenar macula) were embedded in Araldite 502. Semithin sections (1 μ) were stained with toluidine blue.

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