INFLUENCE OF CYSTEAMINE ON DIFFERENTIAL STAINING OF BUdR-SUBSTITUTED HUMAN CHROMOSOMES

1979 ◽  
Vol 21 (1) ◽  
pp. 145-149 ◽  
Author(s):  
W. Scheid
Keyword(s):  
Visible Light ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Strand Breaks ◽  
Light System

In 5-bromodeoxyuridine (BUdR)-substituted human chromosomes stained with 4′-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.

scholarly journals Differential Staining of Human Chromosomes by Treatment with Urea

1971 ◽  
Vol 47 (9) ◽  
pp. 729-731 ◽  
Author(s):  
Yukimasa SHIRAISHI ◽  
Tosihide H. YOSIDA
Keyword(s):  
Differential Staining ◽  
Human Chromosomes

scholarly journals Enhancement of photo-Fenton catalytic activity with the assistance of oxalic acid on the kaolin–FeOOH system for the degradation of organic dyes

RSC Advances ◽  
2020 ◽  
Vol 10 (32) ◽  
pp. 18704-18714 ◽  
Author(s):  
Chun Xiao ◽  
Su Li ◽  
Fuhao Yi ◽  
Bo Zhang ◽  
Dan Chen ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Oxalic Acid ◽  
Visible Light ◽  
Organic Dyes ◽  
Degradation Mechanism ◽  
Light System ◽  
System P

Degradation mechanism of the K–Fe/oxalic acid/H2O2/visible light system.

scholarly journals Depletion of the bloom syndrome helicase stimulates homology-dependent repair at double-strand breaks in human chromosomes

DNA Repair ◽  
2011 ◽  
Vol 10 (4) ◽  
pp. 416-426 ◽  
Author(s):  
Yibin Wang ◽  
Krissy Smith ◽  
Barbara Criscuolo Waldman ◽  
Alan S. Waldman
Keyword(s):  
Bloom Syndrome ◽  
Double Strand Breaks ◽  
Human Chromosomes ◽  
Strand Breaks

scholarly journals DNA Double-Strand Breaks Coupled with PARP1 and HNRNPA2B1 Binding Sites Flank Coordinately Expressed Domains in Human Chromosomes

PLoS Genetics ◽  
2013 ◽  
Vol 9 (4) ◽  
pp. e1003429 ◽  
Author(s):  
Nickolai A. Tchurikov ◽  
Olga V. Kretova ◽  
Daria M. Fedoseeva ◽  
Dmitri V. Sosin ◽  
Sergei A. Grachev ◽  
...  
Keyword(s):  
Binding Sites ◽  
Double Strand Breaks ◽  
Human Chromosomes ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

scholarly journals Differential Staining of DNA Strand Breaks in Dried Comet Assay Slides

2001 ◽  
Vol 49 (7) ◽  
pp. 921-922 ◽  
Author(s):  
L. Benítez–Bribiesca ◽  
P. Sánchez ◽  
J. Toledo ◽  
R. Peñarroja ◽  
M. Flores ◽  
...  
Keyword(s):  
Comet Assay ◽  
Dna Strand Breaks ◽  
Differential Staining ◽  
Strand Breaks ◽  
Dna Strand

GIEMSA STAINING OF HETEROCHROMATIC AREAS OF HUMAN CHROMOSOMES FOR IDENTIFICATION

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner
Keyword(s):  
Acetic Acid ◽  
Banding Pattern ◽  
Saline Solution ◽  
Simple Technique ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Dna Denaturation ◽  
Quinacrine Fluorescence ◽  
Inexpensive Procedure ◽  
Distinct Banding Pattern

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.

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