Differentiation of Philonema spp. (Nematoda; Philometridae) in British Columbia Salmonid Fishes Using DNA Restriction Fragment Length Differences

1992 ◽  
Vol 49 (8) ◽  
pp. 1650-1656 ◽  
Author(s):  
M. L. Adamson ◽  
D. F. Clease ◽  
L. Margolis
Keyword(s):  
British Columbia ◽  
Restriction Fragment ◽  
Dna Sequences ◽  
Fragment Length ◽  
Sockeye Salmon ◽  
River System ◽  
Restriction Enzymes ◽  
Steelhead Trout ◽  
Banding Patterns ◽  
Restriction Fragment Length

Restriction fragment length differences were compared in Philonema spp. (Nematoda; Philometridae) parasitizing kokanee and sockeye salmon (Oncorhynchus nerka) and rainbow and steelhead trout (Oncorhynchus mykiss) from various British Columbia localities. DNA extracted from individual worms was digested with various restriction enzymes and the resulting fragments were separated by horizontal gel electrophoresis to reveal bands representing cleavage sites within repetitive DNA sequences. Banding patterns reveal that worms from the two host species represent distinct genetic stocks. Kokanee and sockeye salmon from all localities sampled harboured the same stock of Philonema, identified as P. oncorhynchi. Worms from rainbow and steelhead trout were clearly distinguished from P. oncorhynchi and are assigned to P. agubernaculum. Philonema aguhernaculum from different localities were distinguishable by their banding patterns; material from Pennask Lake resembled that from Babine Lake and could be distinguished from that from O'Connor Lake on Vancouver Island. This similarity may indicate a common postglacial ancestor for Pennask Lake and Babine Lake worms, perhaps by postglacial colonization from the Columbia River system.

scholarly journals Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 196 ◽  
Author(s):  
García-Suárez ◽  
González-Rodríguez ◽  
Cima-Cabal ◽  
Yuste ◽  
Vazquez ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Dna Sequences ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Pcr Rflp ◽  
Restriction Fragment Length

Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes for Restriction Fragment Length Polymorphism-RFLP typing, in order to maximize the discrimination between different serotypes. DNA sequences of 90 serotypes for the region between dexB and aliA genes were compiled, and a computer screening of restriction enzymes was performed. The wzg–wzh–wzd–wze region and Sse9I restriction predicted unique PCR-RFLP patterns for 39 serotypes and eight serogroups. A second restriction enzyme resolved fragment specific patterns for 25 serotypes. The method was tested with 98 serotype-unknown clinical isolates. PCR-RFLP analysis deduced correct serotypes that were confirmed by Quellung reaction for 78.5% of the isolates.

Characterization of Aeromonas salmonicida Strains using DNA Probe Technology

1989 ◽  
Vol 46 (5) ◽  
pp. 877-879 ◽  
Author(s):  
Martha Hennigan ◽  
Laurence M. Vaughan ◽  
Timothy J. Foster ◽  
Peter Smith ◽  
Frank Gannon
Keyword(s):  
Restriction Fragment ◽  
Causative Agent ◽  
Dna Sequences ◽  
Fragment Length ◽  
Restriction Enzymes ◽  
Dna Probes ◽  
Aeromonas Salmonicida ◽  
Different Strains ◽  
Restriction Fragment Length

Epizootiological studies on Aeromonas salmonicida are important in view of its role as the causative agent of furunculosis. The use of DNA probes to detect restriction fragment length variations promised to provide a new way to distinguish between different strains of the organism. We used four different DNA probes in combination with seven restriction enzymes to compare seven strains of A. salmonicida. Despite the diversity of the geographical origins of the organisms no differences in the patterns of the hybridizing bands were found. This suggests that the DNA sequences of A. salmonicida are very strongly conserved and that the potential for DNA probes to identify different strains may be limited.

Phylogenetic relationships of the pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphisms

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 216-223 ◽  
Author(s):  
Ram G. Nadimpalli ◽  
R. L. Jarret ◽  
Sharad C. Phatak ◽  
Gary Kochert
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Phylogenetic Relationships ◽  
Seed Protein ◽  
Restriction Enzymes ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Genomic Probes ◽  
Clustering Patterns ◽  
Restriction Fragment Length

Nuclear restriction fragment length polymorphisms (RFLPs) were used to determine phylogenetic relationships in the genus Cajanus using 15 random genomic probes and six restriction enzymes. Twenty-four accessions representing 12 species of four genera (Cajanus, Dunbaria, Eriosema, and Rhynchosia) were examined to determine phylogenetic relationships in the genus Cajanus. Eriosema parviflorum was selected as the out-group. Sufficient RFLP polymorphisms were detected among species to resolve in-group taxa into distinct clusters. Topologies of trees from parsimony and similarity matrix analyses were similar but not identical, and clustering patterns agreed broadly with published phylogenies based on seed protein data and, to a lesser extent, data from cytology and breeding experiments. Accessions of cultivated C. cajan shared more DNA fragments with C. scarabaeoides than with C. cajanifolia. Inconsistencies in taxonomic relationships based on data from morphology, cytology, crossability, and RFLPs are discussed.Key words: pigeonpea, systematics, taxonomy, evolution, germplasm.

Restriction fragment length polymorphism analysis of rotavirus VP7-encoding gene from humans and animals of Northeast India: a relative study of Indian and global isolates

2015 ◽  
Vol 143 (12) ◽  
pp. 2503-2511 ◽  
Author(s):  
P. CHAKRABORTY ◽  
N. N. BARMAN ◽  
I. SHARMA
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Northeast India ◽  
Polymorphism Analysis ◽  
Indian Isolates ◽  
Restriction Fragment Length

SUMMARYA restriction fragment length polymorphism (RFLP) assay was developed to examine the genetic relationship between 67 (29 Indian, 38 global) rotavirus isolates of human, bovine and porcine neonates. The assay involved direct digestion of RT–PCR amplified VP7 cDNAs with three restriction enzymes (VspI,HaeIII,NlaIV) independently. Forty-eight RFLP patterns were identified for all 67 strains, and of these 20 patterns were associated with Indian isolates. A correlation between the restriction patterns and G type was apparent through deduction of enzyme restriction sites from known sequences. Major G serotypes (G1, G2, G6, G8) with a few mixed types could be differentiated where there was a positive assortment of intrinsic serotypes from multiple host origin, and certain single or combined enzyme profiles were highly dominant in the population. Significant genetic variations were established between global and Indian isolates and none of the RFLP patterns were shared between them. These data suggest that the Indian wild-type rotavirus population is distinguishable based on the VP7 gene, and co-circulation of distinct strains in different hosts is foremost, indicating the possible likelihood of inter-species transmission.

Comparison of restriction fragment length polymorphisms in wild and cultivated barley

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 298-306 ◽  
Author(s):  
M. A. Saghai Maroof ◽  
Ruslan Biyashev ◽  
Qifa Zhang
Keyword(s):  
Hordeum Vulgare ◽  
Restriction Fragment ◽  
Dna Sequences ◽  
Fragment Length ◽  
Natural Populations ◽  
Single Copy ◽  
Diversity Indices ◽  
Relative Level ◽  
Cultivated Barley ◽  
Restriction Fragment Length

This study was undertaken to assess the relative level of molecular diversity between cultivated barley, Hordeum vulgare ssp. vulgare (HV), and one of its wild relatives, H. vulgare ssp. spontaneum (HS), and to identify possible restriction fragment length polymorphism (RFLP) patterns that may provide information concerning the phylogenetic relationship between these two barley groups. A total of 363 barley accessions were assayed, including 95 entries of HV collected from 36 major barley growing countries of the world and 268 entries of HS from 25 natural populations in Israel and Iran. The 26 RFLP marker loci used in the survey represent single-copy, low-copy, and repetitive DNA sequences and mark all of the chromosome arms. A randomization test, on the basis of equal sample sizes, showed that HS is more polymorphic than HV, as evaluated by the number of alleles and diversity indices. The analysis also indicated extensive RFLP differentiation between these two barley groups; highly significant differences of allele frequencies were detected at the majority of the loci. The HV sample can be subdivided according to winter or spring growth habits, and two- or six-rowed spikes. Analysis of genetic polymorphisms in these subgroups showed that levels of diversity were about equal in spring and winter groups and also in the groups with two- and six-rowed spikes. However, significant differences of allelic frequencies were detected between subgroups of the two divisions.Key words: Hordeum vulgare, genetic diversity, germplasm.

scholarly journals Restriction Fragment Length Polymorphism Analysis Using Random Chromosomal Gene Probes for Epidemiological Analysis ofCampylobacter jejuni Infections

2000 ◽  
Vol 38 (4) ◽  
pp. 1664-1667 ◽  
Author(s):  
Shuji Fujimoto ◽  
Kenichi Umene ◽  
Mitsumasa Saito ◽  
Kazumi Horikawa ◽  
Martin J. Blaser
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Enzymes ◽  
Polymorphism Analysis ◽  
Chromosomal Gene ◽  
Epidemiological Analysis ◽  
Gene Probes ◽  
Length Polymorphisms ◽  
Ofcampylobacter Jejuni ◽  
Restriction Fragment Length

We have evaluated the ability of a new genotyping method forCampylobacter jejuni based on restriction fragment length polymorphisms using random chromosomal gene probes. DNAs from C. jejuni strains digested with each of three restriction enzymes,HhaI, HaeIII, and HpaII, were analyzed by Southern hybridization using each of two unrelated cosmid clones, P14 and P15 (respectively containing 30- and 35-kb genomic DNA fragments of C. jejuni strain OH4384). The method reported provides a stable and discriminating means for identifying C. jejuni strains and should be useful for epidemiological analyses.

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