Characterization of Aeromonas salmonicida Strains using DNA Probe Technology

1989 ◽  
Vol 46 (5) ◽  
pp. 877-879 ◽  
Author(s):  
Martha Hennigan ◽  
Laurence M. Vaughan ◽  
Timothy J. Foster ◽  
Peter Smith ◽  
Frank Gannon
Keyword(s):  
Restriction Fragment ◽  
Causative Agent ◽  
Dna Sequences ◽  
Fragment Length ◽  
Restriction Enzymes ◽  
Dna Probes ◽  
Aeromonas Salmonicida ◽  
Different Strains ◽  
Restriction Fragment Length

Epizootiological studies on Aeromonas salmonicida are important in view of its role as the causative agent of furunculosis. The use of DNA probes to detect restriction fragment length variations promised to provide a new way to distinguish between different strains of the organism. We used four different DNA probes in combination with seven restriction enzymes to compare seven strains of A. salmonicida. Despite the diversity of the geographical origins of the organisms no differences in the patterns of the hybridizing bands were found. This suggests that the DNA sequences of A. salmonicida are very strongly conserved and that the potential for DNA probes to identify different strains may be limited.

scholarly journals Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 196 ◽  
Author(s):  
García-Suárez ◽  
González-Rodríguez ◽  
Cima-Cabal ◽  
Yuste ◽  
Vazquez ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Dna Sequences ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Pcr Rflp ◽  
Restriction Fragment Length

Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes for Restriction Fragment Length Polymorphism-RFLP typing, in order to maximize the discrimination between different serotypes. DNA sequences of 90 serotypes for the region between dexB and aliA genes were compiled, and a computer screening of restriction enzymes was performed. The wzg–wzh–wzd–wze region and Sse9I restriction predicted unique PCR-RFLP patterns for 39 serotypes and eight serogroups. A second restriction enzyme resolved fragment specific patterns for 25 serotypes. The method was tested with 98 serotype-unknown clinical isolates. PCR-RFLP analysis deduced correct serotypes that were confirmed by Quellung reaction for 78.5% of the isolates.

Differentiation of Philonema spp. (Nematoda; Philometridae) in British Columbia Salmonid Fishes Using DNA Restriction Fragment Length Differences

1992 ◽  
Vol 49 (8) ◽  
pp. 1650-1656 ◽  
Author(s):  
M. L. Adamson ◽  
D. F. Clease ◽  
L. Margolis
Keyword(s):  
British Columbia ◽  
Restriction Fragment ◽  
Dna Sequences ◽  
Fragment Length ◽  
Sockeye Salmon ◽  
River System ◽  
Restriction Enzymes ◽  
Steelhead Trout ◽  
Banding Patterns ◽  
Restriction Fragment Length

Restriction fragment length differences were compared in Philonema spp. (Nematoda; Philometridae) parasitizing kokanee and sockeye salmon (Oncorhynchus nerka) and rainbow and steelhead trout (Oncorhynchus mykiss) from various British Columbia localities. DNA extracted from individual worms was digested with various restriction enzymes and the resulting fragments were separated by horizontal gel electrophoresis to reveal bands representing cleavage sites within repetitive DNA sequences. Banding patterns reveal that worms from the two host species represent distinct genetic stocks. Kokanee and sockeye salmon from all localities sampled harboured the same stock of Philonema, identified as P. oncorhynchi. Worms from rainbow and steelhead trout were clearly distinguished from P. oncorhynchi and are assigned to P. agubernaculum. Philonema aguhernaculum from different localities were distinguishable by their banding patterns; material from Pennask Lake resembled that from Babine Lake and could be distinguished from that from O'Connor Lake on Vancouver Island. This similarity may indicate a common postglacial ancestor for Pennask Lake and Babine Lake worms, perhaps by postglacial colonization from the Columbia River system.

Restriction fragment length polymorphisms demonstrate single origin of infection centers in Phellinus weirii

1994 ◽  
Vol 72 (4) ◽  
pp. 440-447 ◽  
Author(s):  
Hanhong Bae ◽  
Everett M. Hansen ◽  
Steven H. Strauss
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Host Species ◽  
Root Rot ◽  
Biological Species ◽  
Douglas Fir ◽  
Dna Probes ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) markers were used to study genetic variation in the basidiomycete fungus Phellinus weirii (Murr.) Gilbertson, the cause of laminated root rot of conifers. In an initial study, three isolates each from the Douglas-fir type and the cedar-type biological species were surveyed with 12 restriction enzymes and 20 random, mitochondrial, and nuclear-ribosomal gene probes. The two biological species were distinct with most probe–enzyme combinations (91%). Variation within biological species was detected for the random and ribosomal DNA probes but not for the mitochondrial DNA probes. In a subsequent study 65 probe–enzyme combinations (13 × 5) that had detected variation within the Douglas-fir type biological species were used to analyze 27 isolates derived from six infection centers, two host species, and two geographic areas in western Oregon. Infection centers differed from one another in numerous probe–enzyme combinations but were nearly genetically uniform within. Isolates from the two host species, Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and mountain hemlock (Tsuga mertensiana (Bong.) Carr.), showed few RFLP differences. Initiation of infection centers, and subsequent vegetative or basdiospore initiated immigration, appear to be rare events. Key words: ribosomal DNA, mitochondrial DNA, RFLP, root rot, conifer.

Characterization of DR blank alleles by restriction fragment length polymorphism (RFLP)

2008 ◽  
Vol 29 (3) ◽  
pp. 154-159 ◽  
Author(s):  
E. Keller ◽  
A. Andreas-Zietz ◽  
A. McNicholas ◽  
A. Grooms ◽  
S. Scholz ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length
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