Effect of different restriction enzymes, probe source, and probe length on detecting restriction fragment length polymorphism in tomato

1990 ◽  
Vol 80 (3) ◽  
pp. 385-389 ◽  
Author(s):  
J. C. Miller ◽  
S. D. Tanksley
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Probe Length ◽  
Probe Source ◽  
Restriction Fragment Length

scholarly journals Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 196 ◽  
Author(s):  
García-Suárez ◽  
González-Rodríguez ◽  
Cima-Cabal ◽  
Yuste ◽  
Vazquez ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Dna Sequences ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Pcr Rflp ◽  
Restriction Fragment Length

Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes for Restriction Fragment Length Polymorphism-RFLP typing, in order to maximize the discrimination between different serotypes. DNA sequences of 90 serotypes for the region between dexB and aliA genes were compiled, and a computer screening of restriction enzymes was performed. The wzg–wzh–wzd–wze region and Sse9I restriction predicted unique PCR-RFLP patterns for 39 serotypes and eight serogroups. A second restriction enzyme resolved fragment specific patterns for 25 serotypes. The method was tested with 98 serotype-unknown clinical isolates. PCR-RFLP analysis deduced correct serotypes that were confirmed by Quellung reaction for 78.5% of the isolates.

Restriction fragment length polymorphism analysis of rotavirus VP7-encoding gene from humans and animals of Northeast India: a relative study of Indian and global isolates

2015 ◽  
Vol 143 (12) ◽  
pp. 2503-2511 ◽  
Author(s):  
P. CHAKRABORTY ◽  
N. N. BARMAN ◽  
I. SHARMA
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Northeast India ◽  
Polymorphism Analysis ◽  
Indian Isolates ◽  
Restriction Fragment Length

SUMMARYA restriction fragment length polymorphism (RFLP) assay was developed to examine the genetic relationship between 67 (29 Indian, 38 global) rotavirus isolates of human, bovine and porcine neonates. The assay involved direct digestion of RT–PCR amplified VP7 cDNAs with three restriction enzymes (VspI,HaeIII,NlaIV) independently. Forty-eight RFLP patterns were identified for all 67 strains, and of these 20 patterns were associated with Indian isolates. A correlation between the restriction patterns and G type was apparent through deduction of enzyme restriction sites from known sequences. Major G serotypes (G1, G2, G6, G8) with a few mixed types could be differentiated where there was a positive assortment of intrinsic serotypes from multiple host origin, and certain single or combined enzyme profiles were highly dominant in the population. Significant genetic variations were established between global and Indian isolates and none of the RFLP patterns were shared between them. These data suggest that the Indian wild-type rotavirus population is distinguishable based on the VP7 gene, and co-circulation of distinct strains in different hosts is foremost, indicating the possible likelihood of inter-species transmission.

scholarly journals Differentiation of porcine reproductive and respiratory syndrome virus European vaccine strains from Czech field isolates by restriction fragment length polymorphism analysis of ORF5 gene

2012 ◽  
Vol 47 (No. 10 - 11) ◽  
pp. 295-301 ◽  
Author(s):  
S. Indik ◽  
L. Valíček
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Respiratory Syndrome Virus ◽  
Field Isolates ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) analysis of open reading frame 5 was developed for typing of Czech strains of porcine reproductive and respiratory syndrome virus (PRRSV). The set of restriction enzymes Acc I, Hae II and SnaB I allowed the differentiation of heterogeneous Czech strains of PRRSV clustered separately in the phylogenetic tree. The high-passage strain V-502 (164) was also differentiated from its parent strain V-502. The same restriction enzymes could distinguish the European-type vaccine strains Porcilis PRRS and Pyrsvac-183, registered inCzechRepublic, from the Czech field isolates. The published ORF5 nucleotide sequences allowed us to presume that it will also be possible to distinguish most of European field strains from vaccine strains. PCR-based RFLP analysis can become a valuable tool in epidemiological studies of PRRSV inEurope.

scholarly journals 334 RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN CARROT

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 478d-478
Author(s):  
Vivek Sampath ◽  
Philipp Simon
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Nematode Resistance ◽  
Core Diameter ◽  
Gene Markers ◽  
Restriction Fragment Length

Studies of genetic variation at the DNA level in the genus Daucus have been very limited. Molecular markers based on restriction fragment length polymorphism (RPLP) have been shown to be highly useful and efficient gene markers in other plant species. We have used a total of 20 carrot types (inbreds, varieties, species) for this study. Genomic DNA probes cloned in pGEM (Promega) plasmid of Escherichia coli were hybridized to DNA of these types digested with EcoRI and HindIII restriction enzymes. Based on 50 probe-enzyme combinations we have found RFLP variation to be extensive in Daucus, even among related cultivated genetic stocks. The implications of these results in the germplasm diversity in Daucus will be discussed. Also, a genetic linkage map of carrot will be constructed. The map will be used to determine the genomic regions conditioning traits like root and core diameter, root length, and nematode resistance.

scholarly journals flaBGene as a Molecular Marker for Distinct Identification of Borrelia Species in Environmental Samples by the PCR-Restriction Fragment Length Polymorphism Method

2011 ◽  
Vol 77 (19) ◽  
pp. 7088-7092 ◽  
Author(s):  
Beata Wodecka
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Sequence Similarity ◽  
Restriction Enzymes ◽  
Borrelia Miyamotoi ◽  
Content Type ◽  
Restriction Fragment Length

ABSTRACTA new protocol employing nested PCR-restriction fragment length polymorphism (RFLP) based on theflaBgene and two restriction enzymes was worked out. This protocol allows the identification of allBorreliaspecies transmitted byIxodes ricinusin Europe, includingBorrelia miyamotoiand 3 genetic variants ofB. garinii. A dendrogram offlaBsequence similarity was in accordance with RFLP variants.

Discrimination between Bifidobacterium Species from Human and Animal Origin by PCR–Restriction Fragment Length Polymorphism

2004 ◽  
Vol 67 (6) ◽  
pp. 1284-1288 ◽  
Author(s):  
V. DELCENSERIE ◽  
N. BECHOUX ◽  
T. LÉONARD ◽  
B. CHINA ◽  
G. DAUBE
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Intestinal Flora ◽  
Restriction Enzymes ◽  
Animal Origin ◽  
Bifidobacterium Species ◽  
Restriction Fragment Length

Bifidobacteria are normal intestinal flora in humans and animals. The genus Bifidobacterium includes 31 species of significant host specificity. Taking into account their properties, we proposed to use bifidobacteria as fecal contamination indicators. PCR–restriction fragment length polymorphism on the 16S rDNA gene was used to distinguish the different Bifidobacterium species. Sixty-four strains belonging to 13 different species were differentiated from animal or human origin using one or two restriction enzymes. Moreover, the primers used were specifics of the Bifidobacterium genus. Therefore, this method made it possible to determine both the presence of bifidobacteria in a sample and its origin of contamination.

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