Comparison of restriction fragment length polymorphisms in wild and cultivated barley

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 298-306 ◽  
Author(s):  
M. A. Saghai Maroof ◽  
Ruslan Biyashev ◽  
Qifa Zhang
Keyword(s):  
Hordeum Vulgare ◽  
Restriction Fragment ◽  
Dna Sequences ◽  
Fragment Length ◽  
Natural Populations ◽  
Single Copy ◽  
Diversity Indices ◽  
Relative Level ◽  
Cultivated Barley ◽  
Restriction Fragment Length

This study was undertaken to assess the relative level of molecular diversity between cultivated barley, Hordeum vulgare ssp. vulgare (HV), and one of its wild relatives, H. vulgare ssp. spontaneum (HS), and to identify possible restriction fragment length polymorphism (RFLP) patterns that may provide information concerning the phylogenetic relationship between these two barley groups. A total of 363 barley accessions were assayed, including 95 entries of HV collected from 36 major barley growing countries of the world and 268 entries of HS from 25 natural populations in Israel and Iran. The 26 RFLP marker loci used in the survey represent single-copy, low-copy, and repetitive DNA sequences and mark all of the chromosome arms. A randomization test, on the basis of equal sample sizes, showed that HS is more polymorphic than HV, as evaluated by the number of alleles and diversity indices. The analysis also indicated extensive RFLP differentiation between these two barley groups; highly significant differences of allele frequencies were detected at the majority of the loci. The HV sample can be subdivided according to winter or spring growth habits, and two- or six-rowed spikes. Analysis of genetic polymorphisms in these subgroups showed that levels of diversity were about equal in spring and winter groups and also in the groups with two- and six-rowed spikes. However, significant differences of allelic frequencies were detected between subgroups of the two divisions.Key words: Hordeum vulgare, genetic diversity, germplasm.

scholarly journals Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 196 ◽  
Author(s):  
García-Suárez ◽  
González-Rodríguez ◽  
Cima-Cabal ◽  
Yuste ◽  
Vazquez ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Dna Sequences ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Pcr Rflp ◽  
Restriction Fragment Length

Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes for Restriction Fragment Length Polymorphism-RFLP typing, in order to maximize the discrimination between different serotypes. DNA sequences of 90 serotypes for the region between dexB and aliA genes were compiled, and a computer screening of restriction enzymes was performed. The wzg–wzh–wzd–wze region and Sse9I restriction predicted unique PCR-RFLP patterns for 39 serotypes and eight serogroups. A second restriction enzyme resolved fragment specific patterns for 25 serotypes. The method was tested with 98 serotype-unknown clinical isolates. PCR-RFLP analysis deduced correct serotypes that were confirmed by Quellung reaction for 78.5% of the isolates.

scholarly journals Identification ofBorrelia burgdorferi ospCGenotypes in Host Tissue and Feeding Ticks by Terminal Restriction Fragment Length Polymorphisms

2012 ◽  
Vol 79 (3) ◽  
pp. 958-964 ◽  
Author(s):  
Kimberly Tsao ◽  
Stephen J. Bent ◽  
Durland Fish
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Large Scale ◽  
Natural Populations ◽  
Reservoir Host ◽  
Restriction Fragment Length Polymorphisms ◽  
Eastern United States ◽  
Content Type ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

ABSTRACTWe developed a high-throughput method based on terminal restriction fragment length polymorphisms (T-RFLP) to identifyospCgenotypes from field-collected samples ofBorrelia burgdorferi. We first validated the method by analyzingB. burgdorferi ospCpreviously identified by sequencing. We then analyzed and comparedospCgenotypes detected from ear biopsy tissue from natural populations of the white-footed mouse, a majorB. burgdorferireservoir host species in the eastern United States, and larval ticks feeding on those individual mice. The T-RFLP method enabled us to distinguish all 17ospCgenotypes tested, as well as mixed samples containing more than one genotype. Analysis costs compare favorably to those of alternativeospCidentification methods. The T-RFLP method will facilitate large-scale field studies to advance our understanding of genotype-specific transmission patterns.

Characterization of Hordeum vulgare – Hordeum bulbosum chromosome substitution lines by restriction fragment length polymorphism analysis

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 507-511 ◽  
Author(s):  
Gail M. Timmerman ◽  
Richard A. Pickering ◽  
Gilbert Melz
Keyword(s):  
Hordeum Vulgare ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Molecular Techniques ◽  
Polymorphism Analysis ◽  
Hordeum Bulbosum ◽  
Restriction Fragment Length

Plants obtained from crosses between Hordeum vulgare and H. bulbosum were previously analyzed cytologically and for isozyme composition. They were identified as possessing substitutions of one or more H. vulgare chromosomes by their H. bulbosum homoeologues. To confirm their constitution and assess the merits of molecular techniques, chromosome-specific probes developed for the Triticeae were hybridized to Southern blots of DNA extracted from these plants and their parents. The hybridization patterns in the substitution plants confirmed that particular chromosomes of H. vulgare were replaced by their H. bulbosum homoeologues. For most probes, heterozygosity between pairs of H. bulbosum chromosomes was recorded. A possible duplication involving H. bulbosum homoeologues of barley chromosomes 4 and 7 was observed. Although molecular and cytological methods for analyzing chromosomally engineered plants are complementary, molecular probes may uncover differences not discernible using light microscopy or isozyme analysis.Key words: Hordeum vulgare, Hordeum bulbosum, chromosome substitutions, restriction fragment length polymorphism.

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