In vitro regeneration of plantlets of Scots pine (Pinus sylvestris) with mycorrhizal roots from subcultured callus initiated from needle adventitious buds

1994 ◽  
Vol 72 (8) ◽  
pp. 1144-1150 ◽  
Author(s):  
Supriyanto ◽  
R. Rohr
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
In Vitro Regeneration ◽  
Histological Study ◽  
Axillary Buds ◽  
Adventitious Buds ◽  
Meristematic Tissue ◽  
Hebeloma Cylindrosporum ◽  
Half Strength

Plantlets were regenerated from cultures established from Pinus sylvestris (Scots pine) meristematic tissue. Seedling explants were first stimulated to develop axillary buds. Developing axillary buds produced numerous new meristems that gave rise to globular adventitious buds located along the needles on half-strength modified Murashige and Skoog medium supplemented with coconut milk and 6-benzylaminopurine. A histological study showed that these new buds originated from dedifferentiated mesophyll and epidermal tissues of the needles. Some of these buds were used for the regeneration of whole plantlets, others were excised and transferred to woody plant medium, on which calli developed at the bases of the microcuttings These calli were organogenic when subcultured on a hormone-free medium and initiated a large number of rooted plantlets that showed high potential to multiply themselves indefinitely. This is the first report of regeneration of Scots pine from a subculturable organogenic line. Mycorrhizae were initiated for both types of plantlets with Hebeloma cylindrosporum on a perlite substrate under fully controlled conditions. Mycorrhizae improved the transfer of the plantlets to ex vitro conditions. Key words: organogenesis, mycorrhizae, tissue culture, Pinus sylvestris.

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals Indirect in vitro Regeneration of Viola canescens Wall. ex, Roxb. by using Leaf Calli

2018 ◽  
Vol 28 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Arun Kumar Khajuria ◽  
NS Bisht
Keyword(s):  
Plant Regeneration ◽  
Natural Population ◽  
Plant Tissue ◽  
In Vitro Regeneration ◽  
Soil Condition ◽  
Medicinal Herb ◽  
Growth Chamber ◽  
Half Strength ◽  
Number Of Shoots

An efficient indirect plant regeneration protocol was developed for Viola canescens, an important medicinal herb used in broad spectra of diseases in number of folk medicines since aeon. Excessive use of this plant without any rehabilitating measure has led to decline its natural population. Present investigation reports the use of zeatin to regenerate the plant from the callus on MS following its acclimatization on the soil condition. Calli of the plant responded positively to zeatin and maximum number of shoots 13.07 ± 2.01 were obtained when 9.12 μM concentration of zeatin was used. Regenerated shoots were subsequently rooted with IBA on MS and half strength MS and showed maximum number of roots 14.13 ± 1.64 after 60 days when medium was fortified with 4.92 μM IBA, followed by transferring them to soil condition, acclimatization of the plantlet was carried in growth chamber and then finally to the field for their survival where it showed 80% survival. Plant Tissue Cult. & Biotech. 28(2): 215-222, 2018 (December)

Histological study of organogenesis in vitro from callus cultures of two Nicotiana species

1981 ◽  
Vol 59 (11) ◽  
pp. 1969-1977 ◽  
Author(s):  
V. Nuti Ronchi
Keyword(s):  
Vascular Tissue ◽  
Histological Study ◽  
Parenchyma Cell ◽  
Shoot Formation ◽  
Callus Cultures ◽  
Nicotiana Glauca ◽  
Meristematic Tissue ◽  
Nicotiana Langsdorffii ◽  
Physical And Chemical

The histological events leading to shoot formation in Nicotiana glauca and in the nontumorous Nicotiana glauca × Nicotiana langsdorffii hybrid have been studied. Organized development begins from a single vacuolated parenchyma cell which divides and precociously differentiates tracheidal cells, forming a growth center with nodular structures with xylem in the center and phloem outside. The vascular tissue is precociously separated from the surrounding callus by a layer of cells which are shown to be endodermal by position and by histochemical reactions. Further growth leads to the formation of a mound of meristematic tissue which later forms shoot apical meristems. The sequences of events are discussed in relation to other known systems of regeneration in calluses.The system described could be suitable for evaluating the effects of various physical and chemical agents on the different steps of differentiation.

scholarly journals In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 110-115 ◽  
Author(s):  
A. Sen ◽  
M.M. Sharma ◽  
D. Grover ◽  
A. Batra
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Phyllanthus Amarus ◽  
Regenerated Plants ◽  
Half Strength ◽  
Important Medicinal Plant

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarusDOI: 10.3126/on.v7i1.2557Our Nature (2009) 7:110-115

scholarly journals An efficient in vitro regeneration protocol to generate stable transgenic lines of Black gram (Vigna mungo)

2018 ◽  
Vol 78 (3) ◽  
Author(s):  
Bidyut Kumar Sarmah ◽  
Trishna Konwar ◽  
Borsha Borah ◽  
Arun Kumar Handique ◽  
Sumita Acharjee
Keyword(s):  
Transformation Efficiency ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Vigna Mungo ◽  
Ms Medium ◽  
Black Gram ◽  
Transgenic Lines ◽  
Half Strength ◽  
The Individual

An efficient and quick in vitro regeneration protocol was developed for black gram (Vigna mungo) using wounded embryonic axis with cotyledon as explant. Murashige and Skoog (MS) medium supplemented with 4.44 μM BAP and 2.32 μM Kinetin was found to be effective in producing maximum number (mean 7.80) of multiple shoots. The individual shoots elongated to 4.5 cm when MS medium was supplemented with 2.89 μM GA3 along with 0.44 μM BAP and 0.46 μM KIN. A novel in vitro rooting technique was also optimized for black gram using half-strength liquid MS medium supplemented with 1.34 μM NAA. The shoots in this medium produced the highest number (mean 7.50) of roots with root length of 6.02 cm. The plantlets were transferred to soil mixture and placed in greenhouse where more than 80% successfully grew to maturity. The same protocol was successfully used to generate transgenic black gram lines carrying Bt-Cry2Aa gene through Agrobacteriummediated transformation with a transformation efficiency of 0.42%. The rooted T0 plants grew to maturity and produced T1 seeds with the presence and expression of transgene in T1 plants. Thus, we have standardized an in vitro regeneration protocol suitable for generation of stable transgenic black gram plants.

scholarly journals In vitro organogenesis in Albizia guachapele, Cedrella odorata and Swietenia macrophylla (Fabaceae, Meliaceae)

2015 ◽  
pp. 225-228
Author(s):  
Lisette Valverde Cerdas ◽  
Magali Dufour ◽  
Víctor Villalobos
Keyword(s):  
Latin American ◽  
Culture Medium ◽  
Basal Medium ◽  
Petri Dish ◽  
Adventitious Buds ◽  
Swietenia Macrophylla ◽  
Half Strength ◽  
Aseptic Conditions ◽  
Spanish Cedar

Regeneration of adventitious buds was achieved from hypocotyl explanls of Albizia guachapele (Guayaquil) and Cedrella odorata (Spanish cedar), and from epicotyl explants from Swietenia macrophylla (Honduran Mahogany). Seeds were obtained from CATIE's Latin American Fores! Seed Bank and genninated under aseptic conditions .. Four explants were cultured in each Petri dish on half strength modified Murashige and Skoog basal medium, and five concentrations of BA (benzyladenine) were studied; A. guachapele and S. macrophylla responded positively lo the presence of BA in the culture medium. Otherwise, Cedrella odorata requíred media supplemented with citokinin and auxin combinations lo induce adventitious buds.

scholarly journals In vitro: Influence of Various Concentrations of Plant Growth Regulators (BAP & NAA) and Sucrose on Regeneration of Chenopodium quinoa Willd. Plant

2020 ◽  
pp. 34-43
Author(s):  
Fayza R. Al Gethami ◽  
Hameda El Sayed Ahmed El Sayed
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Chenopodium Quinoa ◽  
Naphthalene Acetic Acid ◽  
Cotyledonary Nodes ◽  
Half Strength ◽  
The Media

In vitro: regeneration of Chenopodium quinoa Willd. was achieved from cotyledonary nodes explants. In this study, used 6-Benzylaminopurine (BAP) and α-Naphthalene Acetic Acid (NAA) of plant growth regulators with different concentrations individually as well as in combination and used different concentrations of sugar (sucrose) with different concentrations. For was rooting, used half strength (½MS), full-strength MS and ½ MS supplemented with 0.2 mg/l of NAA. The results mentioned, explant responding (%) to multiplication was about 73% for all BAP treatments compared with control and average numbers of shoot increased with increased BAP concentration except 5 mg/l of BAP. The highest explant responding (%) was in media supplemented BAP without NAA compared other treatments noted that the media with combination of BAP and NAA gives formation of callus in bases of the plantlets. Also, the result inducted the combinations between (BAP–NAA) was highly significantly (P≤ 0.001) and less effective on number of shoots where the highest number of shoot was 3.40 in media with 3 mg/l BAP compared other treatments. The highest of explant responding 93.33% was in media supplement with 10 g/l sucrose and (10 g/l sucrose + 3 mg/l BAP), but sucrose level for good greening and developed shoots (4 shoots) was in medium supplement with 10 g/l sucrose. The shoots rooted well on half-strength MS medium with 60% percentage of root. The rooted shoots were acclimatized and transferred to green house to follow their development.

scholarly journals In vitro regeneration of two high-yielding eggplant (Solanum melongena L.) varieties of Bangladesh

1970 ◽  
pp. 08-12
Author(s):  
Sabina Yesmin, Mst Muslima Khatun, Tanzena Tanny ◽  
Anica Tasnim Protity ◽  
Md Salimullah ◽  
Iftekhar Alam
Keyword(s):  
In Vitro Regeneration ◽  
Solanum Melongena ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Healthy Plant ◽  
Ms Medium ◽  
Best Response ◽  
Half Strength ◽  
Multiple Shoot Regeneration

An in vitro regeneration protocol was developed for two high-yielding eggplant varieties (Solanum melongena L.) namely BARI begun-4 and BARI begun-6. Multiple shoots were regenerated from cotyledonary explants through organogenesis with growth regulators of different combinations and concentrations.  The best response towards multiple shoot regeneration was achieved from cotyledon explants on MS media complemented with 1 mg/l BAP + 0.2 mg/l IAA in both the two varieties of eggplant. Elongation of shoots was achieved on hormone free MS medium. Regenerated shoots of both the varieties produced   active in vitro root system on half strength of MS medium supplemented with 0.2 mg/l IBA.  The in vitro grown plantlets were acclimatized in soil, grew up to maturity, flowered, fruited and produced seeds as normal healthy plant like the control.

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