PHYSIOLOGICAL AND BIOCHEMICAL STUDIES IN PLANT METABOLISM: VI. THE EFFECT OF ONTOGENY ON THE PHYSIOLOGICAL HETEROGENEITY IN THE FIRST LEAF OF WHEAT

1952 ◽  
Vol 30 (5) ◽  
pp. 558-570 ◽  
Author(s):  
D. W. A. Roberts
Keyword(s):  
Water Content ◽  
Respiration Rate ◽  
Dry Weight ◽  
Weight Basis ◽  
Plant Metabolism ◽  
Mature Leaves ◽  
Biochemical Studies ◽  
Physiological And Biochemical ◽  
Physiological Heterogeneity ◽  
Very High

The respiration rate, R.Q., and the water content of each of the developing quarters of the first leaf of Khapli wheat have been measured at different ages and found to change as the tissue ages. In general the respiration rate declines as the tissue ages. The R.Q. is usually close to unity except in the basal quarter around the fifth day. The water content of the quarters tends to decline as the mature leaves become older. In the basal quarter of four- and five-day-old leaves the oxygen uptake appears to reach its peak when calculated on a dry weight basis. In the five-day-old leaves the rate of water uptake is very high and the R.Q. very low. These properties are thought to be correlated with the phase of elongation which is occurring in this quarter at that stage.

Physiological and biochemical studies in plant metabolism. IV. Effect of illumination and darkness on the physiological heterogeneity in the first leaf of wheat

1951 ◽  
Vol 29 (4) ◽  
pp. 451-466 ◽  
Author(s):  
D.W.A. Roberts
Keyword(s):  
Respiratory Rate ◽  
Nitrogen Content ◽  
Leaf Anatomy ◽  
Soluble Nitrogen ◽  
Plant Metabolism ◽  
Sucrose Content ◽  
Protein Nitrogen ◽  
Biochemical Studies ◽  
Physiological And Biochemical ◽  
Physiological Heterogeneity

The respiratory rate, soluble nitrogen content, protein nitrogen content, water content, reducing substance content, and sucrose content of each of the four quarters of the first leaf of Khapli wheat have been determined after different exposures to light under standardized conditions. The respiratory rate of the different quarters of the leaf as a whole is not determined by the distribution of any of the above mentioned constituents. Protein, water, and sucrose distribution appear to be correlated with the anatomy of the leaf. Leaf anatomy is apparently an important consideration in the physiological heterogeneity of the first leaf of wheat.

Osmoregulation and respiration in a marine chironomid larva, Paraclunio alaskensis Coquillett (Diptera, Chironomidae)

1983 ◽  
Vol 61 (9) ◽  
pp. 1937-1943 ◽  
Author(s):  
Anne Parkinson ◽  
Richard A. Ring
Keyword(s):  
Water Content ◽  
Respiration Rate ◽  
Dry Weight ◽  
Basal Respiration ◽  
Intertidal Habitat ◽  
Chironomid Larva ◽  
Wide Range

The hemolymph of larvae acclimated in freshwater (19 mosmol/L) and seawater (897 mosmol/L) was analyzed and levels of Na+ (191 mM, 247 mM), Mg2+ (8 mM, 14 mM), Cl− (60 mequiv./L, 126 mequiv./L), and osmolarity (417 mosmol/L, 530 mosmol/L) were determined. Paraclunio alaskensis larvae were shown to be both euryhaline and hyperosmotic and hyposmotic regulators. The isotonic point of the hemolymph was 475 mosmol. Whole larvae contained 72% water and actively regulated water content (signified by changes in weight) over a wide range of external media. The basal respiration rate of larvae in seawater (0.032 μg O2∙mg dry weight−1∙h−1) indicated active cuticular respiration. The significance of these findings in relation to the intertidal habitat is discussed.

PHYSIOLOGICAL AND BIOCHEMICAL STUDIES IN PLANT METABOLISM: VIII. THE DISTRIBUTION OF INVERTASE IN THE FIRST LEAF OF KHAPLI WHEAT

1953 ◽  
Vol 31 (4) ◽  
pp. 367-382 ◽  
Author(s):  
D. W. A. Roberts
Keyword(s):  
Maximum Concentration ◽  
Vascular Tissue ◽  
Quantitative Estimation ◽  
Plant Metabolism ◽  
Phloem Parenchyma ◽  
Biochemical Studies ◽  
Wheat Leaf ◽  
Kinetics Of ◽  
Physiological And Biochemical ◽  
Leaf Juice

The kinetics of wheat leaf invertase have been investigated as a basis for its quantitative estimation. No evidence was obtained which indicated that wheat leaf invertase consists of a phosphorylase and a phosphatase. Solid preparations of the enzyme may be made by precipitating the invertase with ammonium sulphate from wheat leaf juice which has been previously freed from chlorophyll by precipitation with acetate buffer at pH 4.5. A study of the distribution of this enzyme in the first leaf of wheat shows that the enzyme reaches its maximum concentration in the leaf just at the close of the period of elongation. The enzyme is more abundant in the basal part of the leaf than the tip and this condition is taken to indicate that the enzyme is associated with the vascular tissue (phloem or phloem parenchyma) rather than the chlorenchyma.

scholarly journals Correlations between Leaf Nutrient Content and the Production of Metabolites in Orange Jessamine (Murraya paniculata L. Jack) Fertilized with Chicken Manure

2015 ◽  
Vol 2 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Siti Rahmah Karimuna ◽  
Sandra Arifin Aziz ◽  
Maya Melati
Keyword(s):  
Bioactive Compounds ◽  
Dry Weight ◽  
Chicken Manure ◽  
Blood Cholesterol ◽  
Mature Leaves ◽  
P Concentration ◽  
Murraya Paniculata ◽  
K Concentration ◽  
Leaf P ◽  
Very High

Plant secondary metabolites are unique sources for pharmaceuticals and food additives. Orange jessamine (Murraya paniculata) contains secondary metabolite that is beneficial to human health including lowering blood cholesterol levels, anti-obesity, and has the antioxidant capacity. Leaves of orange jessamine have several chemical constituents including L-cadinene, methyl-anthranilate, bisabolene, β-caryophyllene, geraniol, Carene, 5-guaiazulene, osthole, paniculatin, tannins, eugenol, citronelled, coumurrayin and coumarin derivatives. This study aimed to determine the correlation between leaf concentrations of N, P and K with leaf bioactive compounds following chicken manure application to the plants. The experiment was conducted at IPB Experimental Station at Cikarawang, Bogor (6o30' - 6o45' S, 106o30'-106o45' E) from March to November 2014 (250 m above sea level). The study used a randomized block design (RBD) with rates of chicken manure as a treatment, i.e. 0, 2.5, 5 and 7.5 kg per plant. Chicken manure was applied at 30 months after planting (MAP). Leaves were harvested by pruning the plants to a height of 75 cm above the soil surface. The results showed that the optimum rates of chicken manure to produce maximum fresh and dry leaf weight were 3.1 kg and 6.5 kg per plant, respectively. This rate was sufficient for leaf production at the first harvest (34 MAP) but was insufficient for the second harvest (38 MAP). K concentration of the leaves from different positions within the plant and leaf age positively correlated with leaf dry weight (P <0.01), but negatively correlated with total flavonoid content (P <0.01). Leaf P concentration was negatively correlated with dry weight of the 5th young leaves, or mature leaves from all positions. Leaf K concentration was categorized very high (3.59-4.10%), whereas leaf P concentration was high (0.28-0.29%) to very high (0.33-0.35%). The 5th mature leaves determined plant K requirements.Keywords: antioxidant, bioactive compounds, leaf position, organic, NPK leaf concentrations

PHYSIOLOGICAL AND BIOCHEMICAL STUDIES IN PLANT METABOLISM: I. THE RESPIRATION OF THE SEEDLING WHEAT LEAF IN STARVATION AND ONTOGENY

1949 ◽  
Vol 27c (3) ◽  
pp. 125-145 ◽  
Author(s):  
George H. Duff ◽  
Dorothy F. Forward
Keyword(s):  
Time Course ◽  
Standard Sample ◽  
Color Changes ◽  
Mature Leaves ◽  
Wheat Leaf ◽  
Temporary Rise ◽  
Pass Through ◽  
Homologous Characters ◽  
Physiological And Biochemical ◽  
Very High

Uniform conditions for the culture of the plants and for conducting starvation–respiration experiments upon the first seedling leaves permit the recapture and hence thorough investigation, of transitory physiological states. A standard sample of isolated, mature leaves, so produced and starved, is shown to be heterogeneous when tested by the tempo at which the tissues in different parts of the sample pass through the color changes that accompany starvation. These are correlated with respiration. Interleaf variation in tempo is relatively small. Intraleaf variation is maximal and the leaf is polarized at isolation but a complex redistribution of tempo within each leaf brings about depolarization and diminishes intraleaf heterogeneity as starvation progresses. The respiration of such a starving sample follows a time course of characteristic pattern, all the prominent features of which have their homologues in the corresponding patterns of younger and older leaves. The homologous characters undergo gradual modification with ontogeny. The respiration of the unstarved isolated leaf is very high and falls rapidly during growth but is low and declines slowly in maturity and senescence. At the transition from growth to maturity a slight, temporary rise in respiration occurs.

scholarly journals Some moisture relations of colloids. II.—Further observations on the evaporation of water from clay and wool

1923 ◽  
Vol 103 (723) ◽  
pp. 664-675 ◽  
Keyword(s):  
Water Content ◽  
Variable Temperature ◽  
Dry Weight ◽  
Vertical Axis ◽  
Weight Basis ◽  
Wool Fabric ◽  
Rate Of Evaporation ◽  
Straight Lines ◽  
Rate Of Movement

In two earlier paper, the results of a study of the rate of evaporation of water from soil, sand and a wool fabric were described and discussed. It was shown that, when the rate of evaporation is plotted against the water content (on a dry weight basis), the curve obtained is made up of three straight lines cutting one another sharply, so that there are points on the curve at which there are sudden changes of direction. The factors determining the shape of the curve were discussed and it was suggested that variable temperature of the drying mass, and two others, due to capillarity, viz., the diminishing rate of movement of water from the interior of the material to the surface as the larger capillary spaces become empty, or as the water wedges between the soil grains get progressively smaller. It was shown that, with the materials mentioned above, the first sloping portion of the rate curves could be accurately expressed by the equation – dw / dt – A = kw in which A is the intercept cut off from the vertical axis when the curve is extrapolated to W = 0. With wool, the curve, on extrapolation, passed through the origin, in which case A = 0.

Control of sprout growth in the potato. Effect of humidity and water supply

1984 ◽  
Vol 62 (10) ◽  
pp. 2140-2145 ◽  
Author(s):  
Gordon I. McIntyre ◽  
William A. Quick
Keyword(s):  
Water Supply ◽  
Water Content ◽  
Significant Interaction ◽  
Growth Response ◽  
Dry Weight ◽  
Weight Basis ◽  
Potato Tubers ◽  
Factors Affecting ◽  
External Water ◽  
Sprout Growth

The growth of apical sprouts of potato tubers, var. Norland, kept in darkness at 15 °C, was significantly increased either by increasing the relative humidity (RH) from approximately 40 to 100% or by supplying water to the basal cut end of the tubers. These two treatments showed a significant interaction, the response to the external water supply being considerably greater at the higher RH. The growth response was closely correlated with increases in the dry weight and the water content of the sprouts, expressed on either a sprout or dry weight basis. The high RH plus water treatment also eliminated the inhibition of sprout growth by light, the length of the sprouts in this treatment being almost 160% greater than in the light at low RH and 60% greater than in the dark. These results are consistent with the hypothesis that when the tuber has no external supply of water, the growth of the sprouts is determined primarily by their water content, and thus by the effect and interaction of factors affecting the supply of water from the tuber and its rate of loss from the sprouts by transpiration.

scholarly journals Physiological and biochemical changes in cocoa seed (Theobroma cacao L.) caused by desiccation Perubahan fisiologi dan biokimia benih kakao (Theobroma cacao L.) akibat desikasi

2016 ◽  
Vol 68 (1) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. RACHMAWATI-HASID ◽  
. NURHAIMI-HARIS ◽  
Tolhas HUTABARAT
Keyword(s):  
Seed Germination ◽  
Water Content ◽  
Theobroma Cacao ◽  
Germination Rate ◽  
Dry Weight ◽  
Maximum Growth ◽  
Growth Potential ◽  
Normal Seedling ◽  
Theobroma Cacao L ◽  
Physiological And Biochemical

 Ringkasan Benih kakao tergolong rekalsitran, benihnya sensitif terhadap desikasi dan apabila disimpan pada kondisi yang menyebabkan kehilangan air, benih akan kehilangan viabilitasnya. Viabilitas benih kakao hanya dapat dipertahankan beberapa hari saja dalam keadaan terbuka pada suhu kamar. Hal ini merupakan kendala dalam penyimpanan dan pengiriman benih kakao. Tujuan penelitian ini adalah untuk menetapkan pengaruh desikasi terhadap karakter fisiologis dan biokimia benih kakao. Benih ICS 60 (kakao lindak) dan DR2 (kakao mulia) diletakkan dalam cawan Petri kemudian disimpan pada suhu 25oC dan Rh 55-75% selama empat hari. Percobaan dilakukan dengan rancang­an petak terpisah, petak utama adalah kandungan air awal dan kritikal. Sebagai anak petak adalah jenis kakao, masing-masing diulang empat kali. Peubah fisiologis yang diukur adalah viabilitas benih mencakup kandungan air benih, potensi tumbuh maksimum, daya berkecambah, kecepatan tumbuh, bobot kering kecambah normal, dan laju pertumbuhan kecambah normal. Di samping itu juga dilakukan pengamatan pola pita protein benih yang dianalisis dengan SDS-PAGE. Kandungan asam absisik (ABA) dan gula stahiosa, raftnosa, glukosa, fruktosa, arabinosa, silosa, serta sukrosa dalam benih yang ditetapkan dengan HPLC Integritas membran benih ditetapkan berdasarkan daya hantar listrik air perendaman benih yang diukur dengan konduktometer. Hasil yang diperoleh menunjukkan bahwa adanya interaksi yang nyata antara desikasi dengan seluruh tolok ukur fisiologis. Desikasi menyebabkan penurunan daya ber­kecambah, bobot kering dan laju pertumbuhan kecambah normal, potensi tumbuh maksimum dan kecepatan tumbuh. Sedang untuk, kandungan ABA, sukrosa, arabinosa dan rafinosa mengalami peningkatan. Di samping itu desikasi menyebabkan dibentuknya protein baru dengan BM 32,5; 47,0 dan 51,0 kDa (DR2); 47,0 dan 51,0 kD (ICS 60). Beberapa protein yang hilang oleh pengaruh desikasi yaitu dengan BM37, 0 (DR2), 19, 0 dan 37, 0 kD (ICS60). Benih ICS60 lebih tahan terhadap desikasi dibandingkan dengan benih DR2. Summary Seed of cocoa is recalcitrant and sensitive to desiccation. In open condition at room temperature, the viability of cocoa seed ultimately lost for several days. These characters are a problem for seed storage and delivery. The objectives of this study are to investigate the effect of desiccation on physi­ological and biochemical characters of cocoa seed. Seeds of ICS 60 (bulk cocoa) and DR2 (fine cocoa) were placed on Petri dishes and stored at 25oC, Rh 55-75% for four days (critical water content). The experiment was conducted with split plot analysis, (1) The main plot was the storage condition initial and critical seeds water content. (2) The sub plot was the variety of cocoa, with four replications of each treatment. The effect of desiccation on seeds viability was tested, based on seed water content, maximum growth potential, seed germination, germination rate, dry weight of normal seedling, and seedling growth rate. Besides, the changes of seed proteins band pattern were also analysed by SDS­PAGE. Abscisic acid, stachyose, raffnose, fructose, arabinose, xyllose, and sucrose seed content were determined by HPLC. The integrity of seed membrane based on the leakage of electrolytes from seeds was measured with a CM 100 multicell conductivity meter. The results showed that there is an interaction with highly significant correlation between desiccation and all of the physiological and biochemical parameters. Desiccation caused the decrease of seed germination, dry weight and growth rate'of normal seedling, maximum growth potential, and germination rate and while the leakage of electrolytes, ABA, sucrose, arabinose and raffinose increased. Besides, desiccation was also caused the formation of new proteins with MW 32.5, 47,0 and 51,0 kDa (DR2); 47,0 and 51,0 kD ICS 60) . On the other hand, several protein were disappeared i.e. MW 37,0 (DR2), 19,0 and 37,0 kD (ICS60). Seeds of ICS 60 are more tolerant to desiccation than seeds of DR2. 

scholarly journals Physiological and biochemical changes in cocoa seed (Theobroma cacao L.) caused by desiccation Perubahan fisiologi dan biokimia benih kakao (Theobroma cacao L.) akibat desikasi

2016 ◽  
Vol 68 (1) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. RACHMAWATI-HASID ◽  
. NURHAIMI-HARIS ◽  
Tolhas HUTABARAT
Keyword(s):  
Seed Germination ◽  
Water Content ◽  
Theobroma Cacao ◽  
Germination Rate ◽  
Dry Weight ◽  
Maximum Growth ◽  
Growth Potential ◽  
Normal Seedling ◽  
Theobroma Cacao L ◽  
Physiological And Biochemical

 Ringkasan Benih kakao tergolong rekalsitran, benihnya sensitif terhadap desikasi dan apabila disimpan pada kondisi yang menyebabkan kehilangan air, benih akan kehilangan viabilitasnya. Viabilitas benih kakao hanya dapat dipertahankan beberapa hari saja dalam keadaan terbuka pada suhu kamar. Hal ini merupakan kendala dalam penyimpanan dan pengiriman benih kakao. Tujuan penelitian ini adalah untuk menetapkan pengaruh desikasi terhadap karakter fisiologis dan biokimia benih kakao. Benih ICS 60 (kakao lindak) dan DR2 (kakao mulia) diletakkan dalam cawan Petri kemudian disimpan pada suhu 25oC dan Rh 55-75% selama empat hari. Percobaan dilakukan dengan rancang­an petak terpisah, petak utama adalah kandungan air awal dan kritikal. Sebagai anak petak adalah jenis kakao, masing-masing diulang empat kali. Peubah fisiologis yang diukur adalah viabilitas benih mencakup kandungan air benih, potensi tumbuh maksimum, daya berkecambah, kecepatan tumbuh, bobot kering kecambah normal, dan laju pertumbuhan kecambah normal. Di samping itu juga dilakukan pengamatan pola pita protein benih yang dianalisis dengan SDS-PAGE. Kandungan asam absisik (ABA) dan gula stahiosa, raftnosa, glukosa, fruktosa, arabinosa, silosa, serta sukrosa dalam benih yang ditetapkan dengan HPLC Integritas membran benih ditetapkan berdasarkan daya hantar listrik air perendaman benih yang diukur dengan konduktometer. Hasil yang diperoleh menunjukkan bahwa adanya interaksi yang nyata antara desikasi dengan seluruh tolok ukur fisiologis. Desikasi menyebabkan penurunan daya ber­kecambah, bobot kering dan laju pertumbuhan kecambah normal, potensi tumbuh maksimum dan kecepatan tumbuh. Sedang untuk, kandungan ABA, sukrosa, arabinosa dan rafinosa mengalami peningkatan. Di samping itu desikasi menyebabkan dibentuknya protein baru dengan BM 32,5; 47,0 dan 51,0 kDa (DR2); 47,0 dan 51,0 kD (ICS 60). Beberapa protein yang hilang oleh pengaruh desikasi yaitu dengan BM37, 0 (DR2), 19, 0 dan 37, 0 kD (ICS60). Benih ICS60 lebih tahan terhadap desikasi dibandingkan dengan benih DR2. Summary Seed of cocoa is recalcitrant and sensitive to desiccation. In open condition at room temperature, the viability of cocoa seed ultimately lost for several days. These characters are a problem for seed storage and delivery. The objectives of this study are to investigate the effect of desiccation on physi­ological and biochemical characters of cocoa seed. Seeds of ICS 60 (bulk cocoa) and DR2 (fine cocoa) were placed on Petri dishes and stored at 25oC, Rh 55-75% for four days (critical water content). The experiment was conducted with split plot analysis, (1) The main plot was the storage condition initial and critical seeds water content. (2) The sub plot was the variety of cocoa, with four replications of each treatment. The effect of desiccation on seeds viability was tested, based on seed water content, maximum growth potential, seed germination, germination rate, dry weight of normal seedling, and seedling growth rate. Besides, the changes of seed proteins band pattern were also analysed by SDS­PAGE. Abscisic acid, stachyose, raffnose, fructose, arabinose, xyllose, and sucrose seed content were determined by HPLC. The integrity of seed membrane based on the leakage of electrolytes from seeds was measured with a CM 100 multicell conductivity meter. The results showed that there is an interaction with highly significant correlation between desiccation and all of the physiological and biochemical parameters. Desiccation caused the decrease of seed germination, dry weight and growth rate'of normal seedling, maximum growth potential, and germination rate and while the leakage of electrolytes, ABA, sucrose, arabinose and raffinose increased. Besides, desiccation was also caused the formation of new proteins with MW 32.5, 47,0 and 51,0 kDa (DR2); 47,0 and 51,0 kD ICS 60) . On the other hand, several protein were disappeared i.e. MW 37,0 (DR2), 19,0 and 37,0 kD (ICS60). Seeds of ICS 60 are more tolerant to desiccation than seeds of DR2. 

scholarly journals PELAPISAN CHITOSAN MENUNDA PERUBAHAN FISIOLOGI DAN BIOKIMIA SERTA

Pro Food ◽  
2017 ◽  
Vol 3 (2) ◽  
pp. 222
Author(s):  
Zainuri . ◽  
Nurrachman . ◽  
Sri Widyastuti ◽  
Daldiri Uluwiyahi
Keyword(s):  
Vitamin C ◽  
Shelf Life ◽  
Water Content ◽  
Respiration Rate ◽  
Confidence Level ◽  
Biochemical Changes ◽  
Chitosan Coating ◽  
Significant Difference ◽  
Soluble Solid ◽  
Physiological And Biochemical

Mango Cv. Gedong Gincu has very good market oppurtunity, but fresh fruit has short shelf life. This research aimed to study the role ofChitosan coating in delaying fruit physiological and biochemical changes and extending the shelf life of mango Cv. Gedong Gincu”. This research was carried out using experimental method with completely randomized design. There were 5 treatments including chitosan 0%, 5%, 10%, 15% and without coating. Assessment for respiration rate, stach content, total soluble solid, vitamin C, wáter content, and texture were carried out on day 0, 4 and 8 of storage. The data were analyses using Analysis ofvarience at 5% confidence level and when significant difference it was further analysed using honestly significance difference at 5% confidence level. The results indicated that chitosan coating resulted in significant difference on respiration rate, starch content, vitamin C, wáter content, texture, but did not affect significantly for the total soluble solid of mango Cv. Gedong Gincu. The physiological and biochemical changes in the fruit that treated with 5% chitosan were much slower compared to the control and chitosan 0% treated fruit. In general, fruit that treated with chitosan coating had longer shelf life (more than 8 days or about 2 weeks) than the control fruit which was only 1 week.   Key words: Mango, physiological and biochemical changes, chitosan, shelf life ABSTRAK Mangga varietas varietas “Gedong Gincu” mempunyai peluang pasar yang sangat bagus, namun buah mangga segar memiliki daya simpan yang rendah. Penelitian ini bertujuan untuk mengkaji peran pelapisan dengan Chitosan dalam penundaaan perubahan fisiologi dan biokomia serta peningkatan daya simpan mangga “Gedong Gincu”.Penelitian ini dilakukan dengan metode experimental dengan Rancangan Acak Lengkap. Terdapat 5 perlakuan konsentrasi chitosan yaitu 0%, 5%, 10%, 15% dan tanpa perlakuan. Parameter yang diamati meliputi laju respirasi, kadar pati, total padatan terlarut (TPT), vitamin C, kadar air dan tekstur.  Pengamatan parameter dilakukan pada hari ke-0, ke-4 dan hari ke-8 penyimpanan. Data hasil pengamatan dianalisis dengan analisis ragam pada taraf nyata 5%. Data dari perlakuan yang berbeda nyata diuji lanjut dengan uji Beda Nyata Jujur pada taraf nyata 5%.Hasil penelitian menunjukkan bahwa perlakuan pelapisan chitosan memberikan pengaruh yang berbeda nyata terhadap laju respirasi, kadar pati, vitamin C, kadar airdan tekstur buah mangga “Gedong Gincu”. Sebaliknya perlakuan chitosan tidak memberikan pengaruh yang berbeda nyata terhadap total padatan terlarut buah mangga Gedong Gincu. Laju perubahan fisiologi dan biokimia buah mangga varietas Gedong Gincu yang dilapisi dengan chitosan 15% lebih lambat dibandingkan dengan perlakuan chitosan 0%, 5%, 10% dan buah yang tidak diperlakukan.  Secara umum buah mangga yang dilapisi dengan chitosan cenderung memiliki daya simpan yang lebih lama (lebih dari 8  hari atau sekitar 2 minggu)dibandingkan dengan buah mangga yang tidak dilapisi dengan chitosan yaitu hanya 1 minggu.   Kata Kunci: Mangga, perubahan fisiologi dan biokimia, chitosan, daya simpan

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