PHYSIOLOGICAL AND BIOCHEMICAL STUDIES IN PLANT METABOLISM: VIII. THE DISTRIBUTION OF INVERTASE IN THE FIRST LEAF OF KHAPLI WHEAT

1953 ◽  
Vol 31 (4) ◽  
pp. 367-382 ◽  
Author(s):  
D. W. A. Roberts
Keyword(s):  
Maximum Concentration ◽  
Vascular Tissue ◽  
Quantitative Estimation ◽  
Plant Metabolism ◽  
Phloem Parenchyma ◽  
Biochemical Studies ◽  
Wheat Leaf ◽  
Kinetics Of ◽  
Physiological And Biochemical ◽  
Leaf Juice

The kinetics of wheat leaf invertase have been investigated as a basis for its quantitative estimation. No evidence was obtained which indicated that wheat leaf invertase consists of a phosphorylase and a phosphatase. Solid preparations of the enzyme may be made by precipitating the invertase with ammonium sulphate from wheat leaf juice which has been previously freed from chlorophyll by precipitation with acetate buffer at pH 4.5. A study of the distribution of this enzyme in the first leaf of wheat shows that the enzyme reaches its maximum concentration in the leaf just at the close of the period of elongation. The enzyme is more abundant in the basal part of the leaf than the tip and this condition is taken to indicate that the enzyme is associated with the vascular tissue (phloem or phloem parenchyma) rather than the chlorenchyma.

Physiological and biochemical studies in plant metabolism. IV. Effect of illumination and darkness on the physiological heterogeneity in the first leaf of wheat

1951 ◽  
Vol 29 (4) ◽  
pp. 451-466 ◽  
Author(s):  
D.W.A. Roberts
Keyword(s):  
Respiratory Rate ◽  
Nitrogen Content ◽  
Leaf Anatomy ◽  
Soluble Nitrogen ◽  
Plant Metabolism ◽  
Sucrose Content ◽  
Protein Nitrogen ◽  
Biochemical Studies ◽  
Physiological And Biochemical ◽  
Physiological Heterogeneity

The respiratory rate, soluble nitrogen content, protein nitrogen content, water content, reducing substance content, and sucrose content of each of the four quarters of the first leaf of Khapli wheat have been determined after different exposures to light under standardized conditions. The respiratory rate of the different quarters of the leaf as a whole is not determined by the distribution of any of the above mentioned constituents. Protein, water, and sucrose distribution appear to be correlated with the anatomy of the leaf. Leaf anatomy is apparently an important consideration in the physiological heterogeneity of the first leaf of wheat.

PHYSIOLOGICAL AND BIOCHEMICAL STUDIES IN PLANT METABOLISM: VI. THE EFFECT OF ONTOGENY ON THE PHYSIOLOGICAL HETEROGENEITY IN THE FIRST LEAF OF WHEAT

1952 ◽  
Vol 30 (5) ◽  
pp. 558-570 ◽  
Author(s):  
D. W. A. Roberts
Keyword(s):  
Water Content ◽  
Respiration Rate ◽  
Dry Weight ◽  
Weight Basis ◽  
Plant Metabolism ◽  
Mature Leaves ◽  
Biochemical Studies ◽  
Physiological And Biochemical ◽  
Physiological Heterogeneity ◽  
Very High

The respiration rate, R.Q., and the water content of each of the developing quarters of the first leaf of Khapli wheat have been measured at different ages and found to change as the tissue ages. In general the respiration rate declines as the tissue ages. The R.Q. is usually close to unity except in the basal quarter around the fifth day. The water content of the quarters tends to decline as the mature leaves become older. In the basal quarter of four- and five-day-old leaves the oxygen uptake appears to reach its peak when calculated on a dry weight basis. In the five-day-old leaves the rate of water uptake is very high and the R.Q. very low. These properties are thought to be correlated with the phase of elongation which is occurring in this quarter at that stage.

PHYSIOLOGICAL AND BIOCHEMICAL STUDIES IN PLANT METABOLISM: VII. THE INVERTASE OF THE SEEDLING WHEAT LEAF

1953 ◽  
Vol 31 (1) ◽  
pp. 81-89 ◽  
Author(s):  
G. M. Ward
Keyword(s):  
Active Material ◽  
Coarse Particles ◽  
Kinetic Characteristics ◽  
Michaelis Constant ◽  
The Press ◽  
Biochemical Studies ◽  
Yeast Invertase ◽  
Wheat Leaf ◽  
Wheat Leaves ◽  
Physiological And Biochemical

The press juice from mature seedling wheat leaves possesses a high thermo-labile activity in the inversion of sucrose. It is concluded that an invertase is present. The active material is not associated with the coarse particles of the protoplasm but is soluble in the sap and in that condition is very stable in the cold. It is, however, sensitive to drying and precipitation. Some kinetic characteristics of this enzyme resemble those of invertases from other sources. But the Michaelis constant is small by comparison with that of yeast invertase and therefore a relatively high affinity of the leaf enzyme for its substrate is indicated.

Elemental Analysis of Phloem Tissue in Lemna Minor Root Tips

1980 ◽  
Vol 38 ◽  
pp. 798-799
Author(s):  
Patrick Echlin ◽  
Thomas Hayes ◽  
Clifford Lai ◽  
Greg Hook
Keyword(s):  
Vascular Tissue ◽  
Lemna Minor ◽  
Atomic Weight ◽  
Companion Cell ◽  
Root Tips ◽  
Phloem Tissue ◽  
Cell Stage ◽  
Phloem Parenchyma ◽  
Parenchyma Cells ◽  
Xylem Element

Studies (1—4) have shown that it is possible to distinguish different stages of phloem tissue differentiation in the developing roots of Lemna minor by examination in the transmission, scanning, and optical microscopes. A disorganized meristem, immediately behind the root-cap, gives rise to the vascular tissue, which consists of single central xylem element surrounded by a ring of phloem parenchyma cells. This ring of cells is first seen at the 4-5 cell stage, but increases to as many as 11 cells by repeated radial anticlinal divisions. At some point, usually at or shortly after the 8 cell stage, two phloem parenchyma cells located opposite each other on the ring of cells, undergo an unsynchronized, periclinal division to give rise to the sieve element and companion cell. Because of the limited number of cells involved, this developmental sequence offers a relatively simple system in which some of the factors underlying cell division and differentiation may be investigated, including the distribution of diffusible low atomic weight elements within individual cells of the phloem tissue.

scholarly journals DUNALIELLA ЗАМГИЙН ФИЗИОЛОГИ, БИОХИМИЙН СУДАЛГААНЫ ДҮН

2016 ◽  
pp. 60-65
Author(s):  
Б Одгэрэл ◽  
Д Цэрэндулам
Keyword(s):  
Protein Content ◽  
Beta Carotene ◽  
Cellular Morphology ◽  
Lag Phase ◽  
Extreme Conditions ◽  
Biotechnological Applications ◽  
Biochemical Studies ◽  
Dry Biomass ◽  
Protein Rich ◽  
Physiological And Biochemical

The genus Dunaliella is widely studied microalgae for its tolerance to extreme conditions, physiological aspects and many biotechnological applications, such as beta-carotene, protein, lipids and many other bioactive compounds. Physiological and biochemical studies are essential to fully explore the properties and possibilities of new isolates of Dunaliella.The aim of this study was to describe cellular morphology, growth rate and protein content of three Dunaliella strains, isolated from salty lakes in Mongolia. The cellular morphology, growth rates, protein contents were studied using microscopic analyses, Neubauer’s chamber, and micro kjeldahl method.Results showed that growths of all three Dunaliella cultures were progressed through lag phase at 3rd day. The growth of Dunaliella D-1 reached its peak on day 6, while Dunaliella D-6 and D-7 reached their stationary phase on day 7. Furthermore, the protein contents of dry biomass in Dunaliella D-1, D-6 and D-7 cultures were 62.2%, 36.1% and 38.15%, respectively. The highest protein content was found in Dunaliella D-1 culture, hence this culture could be used as protein rich supplement in further study.

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