Physiological and biochemical studies in plant metabolism. IV. Effect of illumination and darkness on the physiological heterogeneity in the first leaf of wheat

1951 ◽  
Vol 29 (4) ◽  
pp. 451-466 ◽  
Author(s):  
D.W.A. Roberts

The respiratory rate, soluble nitrogen content, protein nitrogen content, water content, reducing substance content, and sucrose content of each of the four quarters of the first leaf of Khapli wheat have been determined after different exposures to light under standardized conditions. The respiratory rate of the different quarters of the leaf as a whole is not determined by the distribution of any of the above mentioned constituents. Protein, water, and sucrose distribution appear to be correlated with the anatomy of the leaf. Leaf anatomy is apparently an important consideration in the physiological heterogeneity of the first leaf of wheat.

1952 ◽  
Vol 30 (5) ◽  
pp. 558-570 ◽  
Author(s):  
D. W. A. Roberts

The respiration rate, R.Q., and the water content of each of the developing quarters of the first leaf of Khapli wheat have been measured at different ages and found to change as the tissue ages. In general the respiration rate declines as the tissue ages. The R.Q. is usually close to unity except in the basal quarter around the fifth day. The water content of the quarters tends to decline as the mature leaves become older. In the basal quarter of four- and five-day-old leaves the oxygen uptake appears to reach its peak when calculated on a dry weight basis. In the five-day-old leaves the rate of water uptake is very high and the R.Q. very low. These properties are thought to be correlated with the phase of elongation which is occurring in this quarter at that stage.


1952 ◽  
Vol 30 (1) ◽  
pp. 50-66 ◽  
Author(s):  
D. W. A. Roberts

The respiratory rate, soluble nitrogen content, protein nitrogen content, water content, reducing sugar content, and sucrose content of each of the four quarters of the first leaf of Khapli Emmer wheat have been determined for plants grown with different soil water supplies. Under dry conditions the first leaf of wheat contains a higher concentration of nitrogenous substances than it does under moist conditions. The water content of the first leaf of wheat grown in dry soil or very wet soil is lower than it is for leaves grown in moderately moist soil. The significance of these two observations is discussed. Further support has been obtained for the view that the concentration of none of the substances determined is solely responsible for the respiratory rates and gradients observed in the leaves. The role of leaf anatomy in controlling and producing the observed respiratory gradients is discussed.


1951 ◽  
Vol 29 (4) ◽  
pp. 383-402 ◽  
Author(s):  
D. W. A. Roberts

The oxygen uptake and carbon dioxide output records of detached starving first leaves of wheat have been investigated. Small changes in the oxygen tension or carbon dioxide tension of the surrounding air do not make any difference in the records. The data obtained with the Barcroft method agree with those obtained by the Pettenkofer method. The form of the starvation air line is described. It is generally similar in the four quarters of the leaf but the senescent hump of the basal quarter is much flatter and its peak much lower than in the other three quarters. The total senescent hump carbon dioxide is shown to be proportional to the protein nitrogen in each of the four quarters. The effect on the air lines of rubbing and quartering the leaves is described. There is a great temporary increase in carbon dioxide output as a result of rubbing. This quickly subsides and the respiration rate after the rubbing settles down to a value slightly in excess of that prior to the rubbing. Rubbing and quartering have no effect on the R.Q. of the starving leaves.


1953 ◽  
Vol 31 (4) ◽  
pp. 367-382 ◽  
Author(s):  
D. W. A. Roberts

The kinetics of wheat leaf invertase have been investigated as a basis for its quantitative estimation. No evidence was obtained which indicated that wheat leaf invertase consists of a phosphorylase and a phosphatase. Solid preparations of the enzyme may be made by precipitating the invertase with ammonium sulphate from wheat leaf juice which has been previously freed from chlorophyll by precipitation with acetate buffer at pH 4.5. A study of the distribution of this enzyme in the first leaf of wheat shows that the enzyme reaches its maximum concentration in the leaf just at the close of the period of elongation. The enzyme is more abundant in the basal part of the leaf than the tip and this condition is taken to indicate that the enzyme is associated with the vascular tissue (phloem or phloem parenchyma) rather than the chlorenchyma.


1958 ◽  
Vol 36 (8) ◽  
pp. 799-803 ◽  
Author(s):  
D. W. Duncan ◽  
H. L. A. Tarr

The nitrogen content of three protein fractions and one non-protein nitrogen fraction of muscles of migrating sockeye salmon was determined at three points on the migratory route. The results have been expressed as milligrams of nitrogen per 100 g. of muscle, as per cent of the total muscle nitrogen, and as grams of nitrogen per average (standard) male or female fish.


1958 ◽  
Vol 36 (1) ◽  
pp. 799-803 ◽  
Author(s):  
D. W. Duncan ◽  
H. L. A. Tarr

The nitrogen content of three protein fractions and one non-protein nitrogen fraction of muscles of migrating sockeye salmon was determined at three points on the migratory route. The results have been expressed as milligrams of nitrogen per 100 g. of muscle, as per cent of the total muscle nitrogen, and as grams of nitrogen per average (standard) male or female fish.


Author(s):  
Б Одгэрэл ◽  
Д Цэрэндулам

The genus Dunaliella is widely studied microalgae for its tolerance to extreme conditions, physiological aspects and many biotechnological applications, such as beta-carotene, protein, lipids and many other bioactive compounds. Physiological and biochemical studies are essential to fully explore the properties and possibilities of new isolates of Dunaliella.The aim of this study was to describe cellular morphology, growth rate and protein content of three Dunaliella strains, isolated from salty lakes in Mongolia. The cellular morphology, growth rates, protein contents were studied using microscopic analyses, Neubauer’s chamber, and micro kjeldahl method.Results showed that growths of all three Dunaliella cultures were progressed through lag phase at 3rd day. The growth of Dunaliella D-1 reached its peak on day 6, while Dunaliella D-6 and D-7 reached their stationary phase on day 7. Furthermore, the protein contents of dry biomass in Dunaliella D-1, D-6 and D-7 cultures were 62.2%, 36.1% and 38.15%, respectively. The highest protein content was found in Dunaliella D-1 culture, hence this culture could be used as protein rich supplement in further study.


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