Analysis of Underivatized Brassinosteroids by HPLC/APCI-MS. Occurrence of 3-Epibrassinolide in Arabidopsis thaliana

2001 ◽  
Vol 66 (12) ◽  
pp. 1729-1734 ◽  
Author(s):  
Olga V. Konstantinova ◽  
Andrey P. Antonchick ◽  
Neil J. Oldham ◽  
Vladimir N. Zhabinskii ◽  
Vladimir A. Khripach ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Metabolic Pathways ◽  
Plant Material ◽  
Suspension Cultures ◽  
Ms Analysis ◽  
Root Callus

In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of Arabidopsis thaliana led to the first identification of 3-epibrassinolide as natural brassinosteroid.

scholarly journals Protocol Optimization of Proteomic Analysis of Korean Ginseng (Panax ginseng Meyer)

Separations ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 53
Author(s):  
Clarissa Braccia ◽  
Bhakti Prinsi ◽  
Mara Colzani ◽  
Alessandra A. Altomare ◽  
Luca Espen ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Proteomic Analysis ◽  
Metabolic Pathways ◽  
Extraction Procedure ◽  
Biotic Stresses ◽  
Phenol Extraction ◽  
Ms Analysis ◽  
Sds Page ◽  
Protein Properties ◽  
Korean Ginseng

The benefits of ginseng have been mainly attributed to its triterpenoids, called ginsenosides. Recent genome sequencing of the Panax ginseng has paved the way for in-depth proteomic studies of this medicinal plant. The current study was conducted to deepen the proteomic information on the root proteome of Korean ginseng. Proteomic workflow was optimized by testing two different strategies, characterized by the phenol extraction procedure, the presence or the absence of SDS-PAGE fractionation step, and nano-scale liquid chromatographic tandem mass spectrometry (nLC-MS/MS) analysis. The results highlighted an evident improvement of proteome extraction by the combination of phenol extraction with SDS-PAGE before the nLC-MS/MS analysis. In addition, a dramatic impact of the steaming process (the treatment to produce red ginseng from ginseng) on protein properties was observed. Overall, the analyses of Korean ginseng permitted the characterization of a total of 2412 proteins. A large number of identified proteins belonged to the functional categories of protein and carbon/energy metabolism (22.4% and 14.6%, respectively). The primary and secondary metabolisms are major metabolic pathways, which emerged from the proteomic analysis. In addition, a large number of proteins known to play an important role in response to (a)biotic stresses were also identified. The current proteomic study not only confirmed the previous transcriptomic and proteomic reports but also extended proteomic information, including the main metabolic pathways involved in Korean ginseng.

scholarly journals UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

2021 ◽  
Vol 72 (1) ◽  
pp. 97-108
Author(s):  
Rafeef K. Massadeh ◽  
Tamam El-Elimat ◽  
Mohammad Al-Gharaibeh ◽  
Khaled Tawaha ◽  
Feras Q. Alali
Keyword(s):  
High Resolution ◽  
Plant Material ◽  
Methanolic Extract ◽  
Volatile Oil ◽  
Ethyl Linoleate ◽  
Major Constituent ◽  
Hexadecanoic Acid ◽  
Nicotiana Glauca ◽  
Plant Oil ◽  
Ms Analysis

Abstract The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.

scholarly journals “Pharmaco-chemical Characterization and Evaluation of In Vitro Antioxidant and Antidiabetic Activity of Ethanolic Flower Extract of Clerodendrum paniculatum”

2020 ◽  
Vol 11 (3) ◽  
pp. 4569-4577
Author(s):  
Sincy Varghese ◽  
Kanakasabapathi Devaki ◽  
Poornima Kannappan ◽  
Sri Rashmy Madathil
Keyword(s):  
Secondary Metabolites ◽  
Plant Material ◽  
Quantitative Estimation ◽  
Chemical Characterization ◽  
Antidiabetic Activity ◽  
Flower Extract ◽  
Ms Analysis ◽  
Ic50 Value ◽  
Biochemical Features

Quantitative and qualitative analysis of different components and antioxidant and activities of the extract of flower (CPF) were analyzed in . identification of of flower was also identified by GC-MS analysis. assess the biochemical features of CPF. solvent extraction of CPF was performed using solvents in increasing order of polarity (petroleum ether, chloroform, ethyl acetate, ethanol, and water) and solvent with maximum profile was standardized for further analysis. Quantitative analysis of selected secondary metabolites like tannin, , alkaloids, and of the flower extract was done by UV . In antioxidant assays and in efficacy of the flower extract were analyzed by respective in assays. identification of in CPF was identified by using GC-MS analysis revealed secondary metabolites in the extract, and further analysis of the extract was performed. Quantitative estimation revealed an accountable amount of secondary metabolites like (47.66mg/g acid equivalent), (24mg/g equivalent), (41mg/g equivalent), and alkaloids (1.79mg/g of extracted plant material). analysis (GC-MS) also confirmed convincing compounds in the extract. From in antioxidant and assay, the IC50 value of the extract of CPF was measured and compared with standard, and from the results, it was evident that the extract had significant in antioxidant and activity. the above results, it can be confirmed thatCPF has got significant and therapeutically active ingredients, as evident in analysis. This is further supported by considerable antioxidant and properties observed in respective assays.

scholarly journals New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach

2019 ◽  
Vol 20 (4) ◽  
pp. 814 ◽  
Author(s):  
Inmaculada Coleto ◽  
Izargi Vega-Mas ◽  
Gaetan Glauser ◽  
María González-Moro ◽  
Daniel Marino ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Metabolic Pathways ◽  
Inorganic Nitrogen ◽  
Essential Element ◽  
Nitrous Oxide Emissions ◽  
Stressful Situation ◽  
Ph Homeostasis ◽  
Proteomic Approach ◽  
Wide Range ◽  
Ammonium Nutrition

Nitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertilization has been shown less environmentally harmful compared to nitrate fertilization, because of reducing, among others, nitrate leaching and nitrous oxide emissions. However, ammonium nutrition may become a stressful situation for a wide range of plant species when the ion is present at high concentrations. Although studied for long time, there is still an important lack of knowledge to explain plant tolerance or sensitivity towards ammonium nutrition. In this context, we performed a comparative proteomic study in roots of Arabidopsis thaliana plants grown under exclusive ammonium or nitrate supply. We identified and quantified 68 proteins with differential abundance between both conditions. These proteins revealed new potential important players on root response to ammonium nutrition, such as H+-consuming metabolic pathways to regulate pH homeostasis and specific secondary metabolic pathways like brassinosteroid and glucosinolate biosynthetic pathways.

Biotechnological Production of Natural Calorie Free Steviol Glycosides in Stevia rebaudiana: An Update on Current Scenario

2020 ◽  
Vol 8 (2) ◽  
pp. 70-84
Author(s):  
Abeer Kazmi ◽  
Mubarak Ali Khan ◽  
Sher Mohammad ◽  
Amir Ali ◽  
Huma Ali
Keyword(s):  
Medicinal Plant ◽  
Cell Cultures ◽  
Metabolic Pathways ◽  
Plant Material ◽  
Research Work ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Market Demand ◽  
Biotechnological Production ◽  
Current Scenario

Stevia rebaudiana is a vital medicinal plant of the genus Stevia and family Asteraceae. It is commonly used as a natural sweetener plant and its products are 300 times sweeter than the commonly used sugarcane. The sweetening potential is due to the presence of calorie-free steviol glycosides (SGs). The plant species has been extensively profiled to identify steviol glycosides (SGs) with intensity sweetening properties. However, the limited production of plant material is not fulfilling the higher market demand worldwide. Researchers are working worldwide to enhance the production of important SGs through the intervention of different biotechnological approaches in S. rebaudiana. In this review, the research work conducted in the last twenty years, on the different aspects of biotechnology to enhance the production of SGs has been precisely reviewed. Biotechnological methods such as micropropagation, callus and cell cultures, elicitation and the metabolomics and transcriptomic elucidation of the biosynthetic metabolic pathways for the production of steviol glycosides have been concisely reviewed and discussed.

Sign in / Sign up

Export Citation Format

Share Document