scholarly journals Protocol Optimization of Proteomic Analysis of Korean Ginseng (Panax ginseng Meyer)

Separations ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 53
Author(s):  
Clarissa Braccia ◽  
Bhakti Prinsi ◽  
Mara Colzani ◽  
Alessandra A. Altomare ◽  
Luca Espen ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Proteomic Analysis ◽  
Metabolic Pathways ◽  
Extraction Procedure ◽  
Biotic Stresses ◽  
Phenol Extraction ◽  
Ms Analysis ◽  
Sds Page ◽  
Protein Properties ◽  
Korean Ginseng

The benefits of ginseng have been mainly attributed to its triterpenoids, called ginsenosides. Recent genome sequencing of the Panax ginseng has paved the way for in-depth proteomic studies of this medicinal plant. The current study was conducted to deepen the proteomic information on the root proteome of Korean ginseng. Proteomic workflow was optimized by testing two different strategies, characterized by the phenol extraction procedure, the presence or the absence of SDS-PAGE fractionation step, and nano-scale liquid chromatographic tandem mass spectrometry (nLC-MS/MS) analysis. The results highlighted an evident improvement of proteome extraction by the combination of phenol extraction with SDS-PAGE before the nLC-MS/MS analysis. In addition, a dramatic impact of the steaming process (the treatment to produce red ginseng from ginseng) on protein properties was observed. Overall, the analyses of Korean ginseng permitted the characterization of a total of 2412 proteins. A large number of identified proteins belonged to the functional categories of protein and carbon/energy metabolism (22.4% and 14.6%, respectively). The primary and secondary metabolisms are major metabolic pathways, which emerged from the proteomic analysis. In addition, a large number of proteins known to play an important role in response to (a)biotic stresses were also identified. The current proteomic study not only confirmed the previous transcriptomic and proteomic reports but also extended proteomic information, including the main metabolic pathways involved in Korean ginseng.

Proteomic analysis of Korean ginseng (Panax ginseng C.A. Meyer)

2005 ◽  
Vol 815 (1-2) ◽  
pp. 147-155 ◽  
Author(s):  
Myung Hee Nam ◽  
Seung Il Kim ◽  
Jang Ryol Liu ◽  
Deok Chun Yang ◽  
Yong Pyo Lim ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Proteomic Analysis ◽  
Korean Ginseng

scholarly journals Proteomic analysis of Korean ginseng(Panax ginseng C. A. Meyer) following exposure to salt stress

2008 ◽  
Vol 35 (3) ◽  
pp. 185-193 ◽  
Author(s):  
Sun-Tae Kim ◽  
Dong-Won Bae ◽  
Kyung-Hee Lee ◽  
Jung-Eun Hwang ◽  
Kyong-Hwan Bang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Panax Ginseng ◽  
Proteomic Analysis ◽  
Korean Ginseng

Comparison of growth characteristics and quality of Korean ginseng (Panax ginseng C.A. Meyer) using different shade materials

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HH Kim ◽  
BJ Seong ◽  
KS Lee ◽  
SI Kim ◽  
JM Geun ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Growth Characteristics ◽  
Korean Ginseng

Identification of ginsenoside markers from dry purified extract of Panax ginseng by a dereplication approach and UPLC–QTOF/MS analysis

2015 ◽  
Vol 109 ◽  
pp. 91-104 ◽  
Author(s):  
Heejung Yang ◽  
Dong Young Lee ◽  
Kyo Bin Kang ◽  
Jeom Yong Kim ◽  
Sun Ok Kim ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Ms Analysis

Label-free Quantitative Proteomic Analysis of Drought-Responsive Proteins in Panax ginseng Meyer

2021 ◽  
Vol 29 (6) ◽  
pp. 369-379
Author(s):  
Ju Young Jung ◽  
Cheol Woo Min ◽  
Hye Won Shin ◽  
Truong Van Nguyen ◽  
Ji hyun Kim ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Proteomic Analysis ◽  
Label Free ◽  
Quantitative Proteomic Analysis

scholarly journals Evaluation of effective protein extraction procedure to profile petiole of Moringa oleifera

2020 ◽  
Vol 7 (2) ◽  
pp. 214
Author(s):  
Zetty Amirah Zulkifli ◽  
Zaidah Rahmat
Keyword(s):  
Moringa Oleifera ◽  
Protein Extraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Extraction Procedure ◽  
Electrophoretic Pattern ◽  
Antioxidant Property ◽  
Extraction Methods ◽  
Protein Profiling ◽  
Sds Page

Moringa oleifera is widely known as multipurpose tree since all of its parts confer multiple functions. The leaf is highly favourable among consumers while the petiole is mostly wasted. There are numerous studies on the flavonoid and antioxidant property of the stem and twig. However, study on the petiole has never been done. There-upon, this study was conducted to develop protein profiling of the petiole. In this study, 6 different protein extraction methods were tested on the fresh petiole before its protein quantity and quality were checked via Bradford assay and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) respectively. The in-solution digestion was then done prior to LC-MS/MS analysis. The protein electrophoretic pattern from the SDS-PAGE proves that method 6 using Tris HCl buffer with incorporation of dithiothreitol (DTT) and phenylmethylsulfonyl fluoride (PMSF) confers the best quality of protein. It produced the highest number of visible individual bands compared to other methods. Meanwhile, 93 proteins were successfully identified via LCMS analysis where the protein, signal response and carbohydrate metabolism categories confer the highest percentage. High quality and content of the protein extracted from the petiole including the antioxidant, anticancer and antidiabetic protein identified suggested that consuming this part of the plant could enhance nutrients of human body.

scholarly journals Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

2016 ◽  
Vol 4 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Yurry Um ◽  
◽  
Mei-Lan Jin ◽  
Ok-Tae Kim ◽  
Young-Chang Kim ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Simple Sequence ◽  
Korean Ginseng
Sign in / Sign up

Export Citation Format

Share Document