The Induction By Laser Light Of The In Vitro Flowering Of Cichorium intybus L. (Long day plant)

1980 ◽  
Author(s):  
Pierre Paulet ◽  
Philippe Badila
Keyword(s):  
Laser Light ◽  
In Vitro Flowering ◽  
Cichorium Intybus ◽  
Long Day

Induction photopériodique de l'organogenèse et de la floraison in vitro chez le Cichorium intybus: aspects histologiques

1992 ◽  
Vol 70 (6) ◽  
pp. 1302-1311 ◽  
Author(s):  
Karima Mikou ◽  
Philippe Badila
Keyword(s):  
Structural Changes ◽  
Cichorium Intybus ◽  
Fluorescent Light ◽  
Flower Formation ◽  
Root Explants ◽  
Subsequent Formation ◽  
Flower Primordia ◽  
Short Day ◽  
Long Day

In root explants of a long-day plant, Cichorium intybus L., grown in vitro, inflorescences were produced under short-day conditions (9 h) if long days were applied on days 10 to 18 of culture. Long days consisted of either 16 h fluorescent light (5.6 W∙m−2) or daily cycles of 9 h white fluorescent plus 15 h red (660 nm, 0.3 W∙m−2) or blue (440 nm, 0.5 W∙m−2) light. The structural changes in the meristems of buds regenerated under these conditions were studied. The influence of photoperiod appeared to be critical between the 10th and 16th days, during the progressive transition from vegetative to prefloral stage, which occurred more rapidly under photoperiodic conditions with red or blue light. In noninductive short days, intermediate meristems could be observed tardily on day 24, but no flower formation took place after transfer to long days. Increasing the quantity of light under short-day conditions up to a level comparable to long-day conditions resulted in a rise in mitotic activity, mainly in the peripheral zone of the meristem, but the vegetative zonation was retained. These data therefore indicate that the duration of light really commits the meristem to the subsequent formation of flower primordia; in contrast, the quantity of light controls the bud formation during the first part of development. Key words: Cichorium, tissue cultures, flowering, meristems, organogenesis, photoperiodism.

scholarly journals REGENERATION OF PLANTS FROM STORED WITLOOF CHICORY FOR SELECTION OF DESIRABLE STORAGE TRAITS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1155b-1155
Author(s):  
Yasseen Mohamed-Yasseen ◽  
Walter E. Splittstoesser
Keyword(s):  
Tissue Culture ◽  
Leaf Explants ◽  
Cichorium Intybus ◽  
Physiological Disorders ◽  
Small Leaf ◽  
Long Day ◽  
Selection Of

A micropropagation procedure was developed to regenerate plants via tissue culture from explants of harvested and stored French endive (Cichorium intybus L. Witloof). The procedure permits the rescue of French endive germplasm that shows resistance to postharvest physiological disorders and diseases. The procedure was used successfully to regenerate plants which showed resistance to different undesirable marketable traits.. Under a long day photoperiod, a high percentage of the explants produced flowers in vitro. Thidiozuron was used successfully to regenerate plants from small leaf explants.

In vitro flowering and fruiting in Portulaca pilosa L.

2021 ◽  
Vol 140 ◽  
pp. 1-3
Author(s):  
Yuping Xiong ◽  
Shuangyan Chen ◽  
Zhenpeng Wei ◽  
Xincheng Yu ◽  
Jinhui Pang ◽  
...  
Keyword(s):  
In Vitro Flowering

scholarly journals Rejuvenation of chicory and lettuce plants following phase change in tissue culture

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Anthony J. Conner ◽  
Helen Searle ◽  
Jeanne M. E. Jacobs
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Seed Set ◽  
Genetic Manipulation ◽  
Adventitious Shoot ◽  
In Vitro Flowering ◽  
Phase Changes ◽  
Adventitious Shoot Regeneration ◽  
Cell And Tissue Culture

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

Photobiomodulation of vascular endothelial and smooth muscle cells in vitro with red laser light

1996 ◽  
Author(s):  
Nicholas Kipshidze ◽  
Michael H. Keelan, Jr. ◽  
Joseph B. Horn ◽  
Victor Nikolaychik
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Laser Light ◽  
Muscle Cells ◽  
Vascular Endothelial
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