In vivo and in vitro flowering response of chicory ( Cichorium intybus L.): influence of plant age and vernalization

1999 ◽  
Vol 18 (9) ◽  
pp. 781-785 ◽  
Author(s):  
M. A. C. Demeulemeester ◽  
M. P. De Proft
Keyword(s):  
In Vitro Flowering ◽  
Cichorium Intybus ◽  
Plant Age ◽  
Flowering Response

Spirulina, Palmaria Palmata, Cichorium Intybus, and Medicago Sativa extracts in cosmetic formulations: an integrated approach of in vitro toxicity and in vivo acceptability studies

2019 ◽  
Vol 38 (4) ◽  
pp. 322-329 ◽  
Author(s):  
Patrícia M. B. G. Maia Campos ◽  
Carolina G. Benevenuto ◽  
Lívia S. Calixto ◽  
Maísa. O. Melo ◽  
Karina C. Pereira ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Integrated Approach ◽  
Cichorium Intybus ◽  
Palmaria Palmata ◽  
In Vitro Toxicity ◽  
Cosmetic Formulations

scholarly journals Micropropagation and Cellular Behaviour Changes During in vitro Flowering of Impatiens balsamina

2019 ◽  
Vol 37 ◽  
Author(s):  
N. MOHAMED ◽  
R.M. TAHA ◽  
U.N.A.A. RAZAK
Keyword(s):  
Chromosome Count ◽  
In Vitro Flowering ◽  
Root Cells ◽  
Ploidy Levels ◽  
Impatiens Balsamina ◽  
Cellular Behaviour ◽  
Behaviour Changes ◽  
Dna Measurements

ABSTRACT: In this study, the micropropagation of Impatiens balsamina was established from stem and shoot explants. The effects of GA3 and glutathione on the morphogenesis of this species were also investigated, in order to induce in vitro flowering. It was found that the optimum in vitro plant regeneration was achieved on MS medium supplemented with 1.0 mg L-1 GA3 and in vitro flowering was also obtained from the same medium after 4 weeks of culture. To understand cellular behavior during in vitro flowering, Mitotic Index (MI), chromosome counts, measurement of mean cell and nuclear areas, DNA measurements and ploidy levels were analyzed from in vivo plants, in vitro grown plants and plantlets that flowered in vitro. The chromosome count was the same for all, 2x=2n=14 or n=7. However, it was observed that in vitro flowering plants of Impatiens balsamina had the highest percentage of polyploid cells (30.7%), based on a histogram plotted by the AxioVision 4.7 software. It was found that plant growth regulators, especially GA3, increased the polyploidy level of the meristematic root cells.

scholarly journals The Role of Plant Growth Regulators in the Development of in vitro Flowering, Histology and Ultrastructural Studies in Impatiens balsamina cv. Dwarf Bush

2018 ◽  
Vol 36 (0) ◽  
Author(s):  
N. MOHAMED ◽  
R.M. TAHA ◽  
U.N.A.A. RAZAK ◽  
H. ELIAS
Keyword(s):  
Plant Growth ◽  
In Vitro Flowering ◽  
Direct Organogenesis ◽  
Stem Explants ◽  
Petiole Explants ◽  
Culture Techniques ◽  
Ultrastructural Studies ◽  
Impatiens Balsamina

ABSTRACT: An efficient protocol for in vitro flowering was successfully established for Impatiens balsamina cv Dwarf Bush, an important medicinal plant, through tissue culture techniques. Shoot, stem and petiole explants obtained from 4 week-old aseptic seedlings cultured on MS medium supplemented with different concentrations of plant growth regulator (PGR) were used for in vitro flower induction. Gibberellic acid (GA3), benzylaminopurine (BAP) and kinetin (Kin) treatment singly applied in MS media (pH 5.8), could all stimulate flowering at 23-26 oC with photoperiod of 16 hours light and 8 hours dark. It was observed that shoot explants were more responsive than stem explants in floral formation. Regeneration was achieved via direct organogenesis. For shoot explants, the treatment that induced the highest rate of in vitro flowering (7.30 ± 0.16 flowers per plantlet) was 1.0 mg L-1 GA3. Ultrastructural and histological analysis of in vivo and in vitro flowers were done to discover any somaclonal variation. This research described a simple protocol for rapid in vitro flowering that will be very beneficial for further breeding, cytological and molecular biology research.

Plant age and in vitro or in vivo propagation considerably affect cold tolerance of Miscanthus×giganteus

2011 ◽  
Vol 34 (3) ◽  
pp. 163-171 ◽  
Author(s):  
A. Płażek ◽  
F. Dubert ◽  
F. Janowiak ◽  
T. Krępski ◽  
M. Tatrzańska
Keyword(s):  
Cold Tolerance ◽  
Plant Age ◽  
Miscanthus Giganteus

scholarly journals In-vitro and in-vivo antioxidant assays of chicory plants (Cichorium intybus L.) as influenced by organic and conventional fertilisers

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Lovro Sinkovič ◽  
Polona Jamnik ◽  
Mojca Korošec ◽  
Rajko Vidrih ◽  
Vladimir Meglič
Keyword(s):  
Cichorium Intybus ◽  
Antioxidant Assays

scholarly journals Cichorium intybus L. Extract Suppresses Experimental Gout by Inhibiting the NF-κB and NLRP3 Signaling Pathways

2019 ◽  
Vol 20 (19) ◽  
pp. 4921 ◽  
Author(s):  
Yu Wang ◽  
Zhijian Lin ◽  
Bing Zhang ◽  
Zhuoxi Jiang ◽  
Fanfan Guo ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Therapeutic Agents ◽  
Cichorium Intybus ◽  
Inflammatory Activity ◽  
Monosodium Urate ◽  
Chicoric Acid ◽  
The Core ◽  
Histopathological Lesion

Background: The production and maturation of interleukin (IL)-1β, regulated by the NF-κB and NLRP3 signaling pathways, lie at the core of gout. This study aimed to evaluate the antigout effect of Cichorium intybus L. (also known as chicory) in vivo and in vitro. Methods: A gout animal model was established with monosodium urate (MSU) crystal injections. Rats were orally administered with chicory extract or colchicine. Levels of ankle edema, inflammatory activity, and IL-1β release were observed. Several essential targets of the NF-κB and NLRP3 signaling pathways were detected. Primary macrophages were isolated to verify the antigout mechanism of chicory extract as well as chicoric acid in vitro. Results: Improvements of swelling degree, inflammatory activity, and histopathological lesion in MSU-injected ankles were observed in the treatment with chicory extract. Further, the chicory extract significantly decreased IL-1β release by suppressing the NF-κB and NLRP3 signaling pathways in gout rats. Similar to the in vivo results, IL-1β release was also inhibited by chicory extract and chicoric acid, a specific effective compound in chicory, through the NF-κB and NLRP3 signaling pathways. Conclusion: This study suggests that chicory extract and chicoric acid may be used as promising therapeutic agents against gout by inhibiting the NF-κB and NLRP3 signaling pathways.

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