Putrescine and Silver Nitrate Influences Shoot Multiplication, In Vitro Flowering and Endogenous Titers of Polyamines in Cichorium intybus L. cv. Lucknow Local

2000 ◽  
Vol 19 (2) ◽  
pp. 238-248 ◽  
Author(s):  
Harsh Pal Bais ◽  
Govinda Swamy Sudha ◽  
Gokare Aswathanarayan Ravishankar
Keyword(s):  
Silver Nitrate ◽  
Shoot Multiplication ◽  
In Vitro Flowering ◽  
Cichorium Intybus

In vitro flowering and shoot multiplication of Gentiana triflora in air-lift bioreactor cultures

2010 ◽  
Vol 12 (01) ◽  
pp. 30-34
Author(s):  
Yaser Hassan Dewir ◽  
Nisha Singh ◽  
Siveshni Govender ◽  
Pragashnee Pillay
Keyword(s):  
Shoot Multiplication ◽  
In Vitro Flowering ◽  
Gentiana Triflora ◽  
Bioreactor Cultures ◽  
Air Lift

scholarly journals Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4945
Author(s):  
O. New Lee ◽  
Gunes Ak ◽  
Gokhan Zengin ◽  
Zoltán Cziáky ◽  
József Jekő ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Somaclonal Variation ◽  
Catharanthus Roseus ◽  
Shoot Multiplication ◽  
In Vitro Flowering ◽  
Phytochemical Composition ◽  
Somaclonal Variant ◽  
Normal Green ◽  
Green Shoot

This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6–1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography–tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.

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