scholarly journals High speed inverted optical-resolution photoacoustic microscopy

2011 ◽  
Author(s):  
Bin Rao ◽  
Konstantin Maslov ◽  
Amos Danielli ◽  
Ruiming Chen ◽  
K. Kirk Shung ◽  
...  
Keyword(s):  
High Speed ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

Real-time functional optical-resolution photoacoustic microscopy using high-speed alternating illumination at 532 and 1064 nm

2017 ◽  
Vol 11 (3) ◽  
pp. e201700210 ◽  
Author(s):  
Heesung Kang ◽  
Sang-Won Lee ◽  
Sang-Min Park ◽  
Soon-Woo Cho ◽  
Jae Yong Lee ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

scholarly journals High-Speed Optical Resolution Photoacoustic Microscopy With a MEMS Scanner: Novel and Simple Distortion Correction Method

2021 ◽  
Author(s):  
Ryo Shintate ◽  
Takuro Ishii ◽  
Joongho Ahn ◽  
Jin Young Kim ◽  
Chulhong Kim ◽  
...  
Keyword(s):  
High Speed ◽  
Optical Resolution ◽  
Calibration Method ◽  
Correction Method ◽  
Distortion Correction ◽  
Photoacoustic Microscopy ◽  
Imaging Tool ◽  
Imaging Sequence ◽  
Biological Interpretation

Abstract Optical resolution photoacoustic microscopy (OR-PAM) is a remarkable biomedical imaging tool that can selectively visualize microtissues with optical-dependent high resolution. However, traditional OR-PAM using mechanical stages provides slow imaging speed, making biological interpretation of in-vivo tissue difficult. Here, we developed a high-speed OR-PAM using a recently commercialized MEMS mirror. This system (MEMS-OR-PAM) consisted of a 1-axis MEMS mirror and a mechanical stage. Furthermore, this study proposed a novel calibration method that quickly removes the spatial distortion caused by fast MEMS scanning. The proposed calibration method needs to run imaging sequence only once using a ruler target and it can easily correct distortions caused by both the scan geometry of the MEMS mirror and its nonlinear motion. The combination of the MEMS-OR-PAM and the distortion correction method was verified by three experiments.; 1) Leaf skeleton phantom imaging to test the distortion correction efficacy.; 2) Spatial resolution and depth of focus (DOF) measurement for the system performance.; 3) In-Vivo finger capillaries imaging to verify their biomedical use. The results showed that the combination could achieve a high-speed (32 sec in 2 mm×4 mm) and high-lateral resolution (~6 µm) imaging capability and precisely visualize the circulating structure of the finger capillaries.

Laser-scanning optical-resolution photoacoustic microscopy

2009 ◽  
Vol 34 (12) ◽  
pp. 1771 ◽  
Author(s):  
Zhixing Xie ◽  
Shuliang Jiao ◽  
Hao F. Zhang ◽  
Carmen A. Puliafito
Keyword(s):  
Laser Scanning ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

scholarly journals High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiufeng Li ◽  
Victor T C Tsang ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T W Wong
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Performance ◽  
Continuous Wave ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
High Signal ◽  
Photoacoustic Microscopy ◽  
Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

scholarly journals High-speed widefield photoacoustic microscopy of small-animal hemodynamics

2018 ◽  
Vol 9 (10) ◽  
pp. 4689 ◽  
Author(s):  
Bangxin Lan ◽  
Wei Liu ◽  
Ya-chao Wang ◽  
Junhui Shi ◽  
Yang Li ◽  
...  
Keyword(s):  
High Speed ◽  
Small Animal ◽  
Photoacoustic Microscopy

scholarly journals High-speed dual-layer scanning photoacoustic microscopy using focus tunable lens modulation at resonant frequency

2017 ◽  
Vol 25 (22) ◽  
pp. 26427 ◽  
Author(s):  
Kiri Lee ◽  
Euiheon Chung ◽  
Seungrag Lee ◽  
Tae Joong Eom
Keyword(s):  
Resonant Frequency ◽  
High Speed ◽  
Photoacoustic Microscopy ◽  
Tunable Lens
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