scholarly journals High-speed widefield photoacoustic microscopy of small-animal hemodynamics

2018 ◽  
Vol 9 (10) ◽  
pp. 4689 ◽  
Author(s):  
Bangxin Lan ◽  
Wei Liu ◽  
Ya-chao Wang ◽  
Junhui Shi ◽  
Yang Li ◽  
...  
Keyword(s):  
High Speed ◽  
Small Animal ◽  
Photoacoustic Microscopy

scholarly journals High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xiufeng Li ◽  
Victor T C Tsang ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T W Wong
Keyword(s):  
High Resolution ◽  
High Speed ◽  
High Performance ◽  
Continuous Wave ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
High Signal ◽  
Photoacoustic Microscopy ◽  
Mouse Ear

AbstractLaser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.

scholarly journals High-speed dual-layer scanning photoacoustic microscopy using focus tunable lens modulation at resonant frequency

2017 ◽  
Vol 25 (22) ◽  
pp. 26427 ◽  
Author(s):  
Kiri Lee ◽  
Euiheon Chung ◽  
Seungrag Lee ◽  
Tae Joong Eom
Keyword(s):  
Resonant Frequency ◽  
High Speed ◽  
Photoacoustic Microscopy ◽  
Tunable Lens

High-speed wide-field photoacoustic microscopy with enhanced sensitivity

2021 ◽  
Author(s):  
Hyojin Kim ◽  
Jin Young Kim ◽  
Jinwoo Baik ◽  
Seonghee Cho ◽  
Chulhong Kim
Keyword(s):  
High Speed ◽  
Wide Field ◽  
Photoacoustic Microscopy ◽  
Enhanced Sensitivity

Design and Performance of a Miniaturized High-Speed Continuous-Flow Analyzer

1974 ◽  
Vol 20 (4) ◽  
pp. 424-427 ◽  
Author(s):  
William E Neeley ◽  
Stephen C Wardlaw ◽  
Helen C Sing
Keyword(s):  
Sample Size ◽  
Continuous Flow ◽  
High Speed ◽  
Clinical Laboratory ◽  
Small Animal ◽  
Sample Volume ◽  
Small Sample ◽  
Reagent Consumption ◽  
And Performance ◽  
Made In

Abstract Design features and performance of a miniaturized high-speed continuous-flow analyzer are described. Special emphasis is made in the design towards a system that is free from the operational and mechanical complexities found in most of today’s advanced systems. Depending on the particular analyses, sample size varies from 3 to 25 µl and reagent consumption is less than 180 µl per sample. Analyses are performed under steady-state conditions at sampling rates of 150 samples per hour with a 2:1 or 3:1 sample-to-wash ratio. The marked reduction in sample size makes the system ideal for microanalyses, especially in the pediatric clinical laboratory, in small animal research, and in any other cases where small sample volume is especially important.

A Comprehensive Approach for Studying Muscle-Tendon Mechanics

1994 ◽  
Vol 116 (1) ◽  
pp. 51-55 ◽  
Author(s):  
D. Hawkins ◽  
M. Bey
Keyword(s):  
High Speed ◽  
Tibialis Anterior Muscle ◽  
Small Animal ◽  
Video Camera ◽  
Comprehensive Approach ◽  
Work Station ◽  
High Speed Video Camera ◽  
Tendon Mechanics ◽  
Animal Muscle ◽  
Intact Muscle

A comprehensive approach for studying the mechanics of partially intact muscle-tendon (MT) complexes was developed. This approach utilizes a work station which integrates state-of-the-art equipment and software. The hardware includes a nerve stimulator, ergometer, high speed video camera and recorder, computer, and temperature regulated chamber. When used in conjunction with a small animal muscle model, the work station provides accurate control of muscle stimulation, MT length, and MT shortening or stretching velocity. Simultaneously, muscle force and both muscle and tendon kinematics can be recorded. This approach is unique in that it allows simultaneous testing of both muscle and tendon under physiological conditions. Additionally, both gross and local deformations of the muscle and tendon can be determined. Sample results from a study of a rat tibialis anterior muscle illustrate the utility of this approach.

High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues

2020 ◽  
Vol 45 (19) ◽  
pp. 5401 ◽  
Author(s):  
Xiufeng Li ◽  
Lei Kang ◽  
Yan Zhang ◽  
Terence T. W. Wong
Keyword(s):  
High Speed ◽  
Biological Tissues ◽  
Photoacoustic Microscopy ◽  
Label Free

scholarly journals High-speed photoacoustic microscopy of mouse cortical microhemodynamics

2016 ◽  
Vol 10 (6-7) ◽  
pp. 792-798 ◽  
Author(s):  
Li Lin ◽  
Junjie Yao ◽  
Ruiying Zhang ◽  
Chun-Cheng Chen ◽  
Chih-Hsien Huang ◽  
...  
Keyword(s):  
High Speed ◽  
Photoacoustic Microscopy

scholarly journals Ultra-high-speed multi-parametric photoacoustic microscopy

2021 ◽  
Author(s):  
Song Hu ◽  
Fenghe Zhong
Keyword(s):  
High Speed ◽  
Laser Scanning ◽  
Ultrasonic Transducer ◽  
Hemoglobin Concentration ◽  
Blood Oxygenation ◽  
Flow Quantification ◽  
Photoacoustic Microscopy ◽  
Ultra High Speed ◽  
Working Distance

Multi-parametric photoacoustic microscopy (PAM) is uniquely capable of simultaneous, high-resolution mapping of blood hemoglobin concentration, oxygenation, and flow in vivo. However, its speed has been limited by the dense sampling required for blood flow quantification. To overcome this limitation, we have developed an ultra-high-speed multi-parametric PAM system, which enables simultaneous acquisition of ~500 densely sampled B-scans by superposing the rapid laser scanning across the line-shaped focus of a cylindrically focused ultrasonic transducer over the conventional mechanical scan of the optical-acoustic dual foci. A novel optical-acoustic combiner is designed and implemented to accommodate the short working distance of the transducer, enabling convenient confocal alignment of the dual foci in the reflection mode. This new system enables continuous monitoring of microvascular hemoglobin concentration, blood oxygenation, and flow over a 4.5 x 3 mm2 area in the awake mouse brain with high spatial and temporal resolution (6.9 μm and 0.3 Hz, respectively).

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