Real-time functional optical-resolution photoacoustic microscopy using high-speed alternating illumination at 532 and 1064 nm

2017 ◽  
Vol 11 (3) ◽  
pp. e201700210 ◽  
Author(s):  
Heesung Kang ◽  
Sang-Won Lee ◽  
Sang-Min Park ◽  
Soon-Woo Cho ◽  
Jae Yong Lee ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

scholarly journals Real-time handheld optical-resolution photoacoustic microscopy

2011 ◽  
Vol 19 (21) ◽  
pp. 20097 ◽  
Author(s):  
Parsin Hajireza ◽  
Wei Shi ◽  
Roger J. Zemp
Keyword(s):  
Real Time ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

scholarly journals Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution

2011 ◽  
Vol 36 (7) ◽  
pp. 1137 ◽  
Author(s):  
Bin Rao ◽  
Konstantin Maslov ◽  
Amos Danielli ◽  
Ruimin Chen ◽  
K. Kirk Shung ◽  
...  
Keyword(s):  
Real Time ◽  
Optical Resolution ◽  
Au Nanoparticle ◽  
Photoacoustic Microscopy

scholarly journals High speed inverted optical-resolution photoacoustic microscopy

2011 ◽  
Author(s):  
Bin Rao ◽  
Konstantin Maslov ◽  
Amos Danielli ◽  
Ruiming Chen ◽  
K. Kirk Shung ◽  
...  
Keyword(s):  
High Speed ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

Real-time optical-resolution photoacoustic microscopy using fiber-laser technology

2011 ◽  
Author(s):  
Wei Shi ◽  
Parsin Hajireza ◽  
Peng Shao ◽  
Shaun Kerr ◽  
Roger J. Zemp
Keyword(s):  
Fiber Laser ◽  
Real Time ◽  
Optical Resolution ◽  
Photoacoustic Microscopy ◽  
Laser Technology

scholarly journals Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy

2022 ◽  
Author(s):  
Junjie Yao ◽  
Xiaoyi Zhu ◽  
Qiang Huang ◽  
Anthony DiSpirito ◽  
Tri Vu ◽  
...  
Keyword(s):  
Real Time ◽  
Oxygen Delivery ◽  
High Speed ◽  
Brain Research ◽  
High Sensitivity ◽  
Wide Field ◽  
Photoacoustic Microscopy ◽  
Whole Brain ◽  
Functional Changes ◽  
Brain Hemodynamics

Abstract High-speed high-resolution imaging of the whole-brain hemodynamics is urgently needed to facilitate the next level of neurovascular research. Image acquisition speed and image quality are crucial to visualizing real-time hemodynamics in complex brain vascular networks, and displaying fast pathophysiological dynamics on a micro and macro-level, enabling advances in current queries in neurovascular and brain metabolism research, including stroke, dementia and acute brain injury. Further, real-time oxygen saturation of hemoglobin (sO2) imaging to differentiate arteries from veins and capture fast-paced oxygen delivery dynamics is needed to solve pertinent questions in these fields and beyond. Here, we present a novel ultrafast functional photoacoustic microscopy (UFF-PAM) to image the whole-brain hemodynamics and oxygen delivery. UFF-PAM takes advantage of several key engineering innovations, including Raman-shifter-based dual-wavelength laser excitation, water-immersible 12-facet-polygon scanner, high-sensitivity ultrasound transducer, and deep-learning-based image upsampling. A volumetric imaging rate of 2 Hz has been achieved over a field of view (FOV) of 11× 7.5 × 1.5 mm3 with a high spatial resolution of ~10 µm. Using the UFF-PAM system, we have demonstrated proof-of-concept functional studies on the mouse brains in response to systemic hypoxia, sodium nitroprusside, and stroke. We observed the mouse brain’s fast morphological and functional changes over the entire cortex, including vasoconstriction, vasodilation, and deoxygenation. More interestingly, for the first time, under the whole-brain FOV and micro-vessel resolution, we captured the vasoconstriction and oxygenation change simultaneously in the spreading depolarization (SD) wave. Our work provides a great potential for fundamental brain research under various pathological and physiological conditions.

scholarly journals In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy

2013 ◽  
Vol 18 (02) ◽  
pp. 1 ◽  
Author(s):  
Wei Shi ◽  
Peng Shao ◽  
Parsin Hajireza ◽  
Alexander Forbrich ◽  
Roger J. Zemp
Keyword(s):  
Real Time ◽  
Dynamic Process ◽  
Optical Resolution ◽  
Photoacoustic Microscopy

scholarly journals High-Speed Optical Resolution Photoacoustic Microscopy With a MEMS Scanner: Novel and Simple Distortion Correction Method

2021 ◽  
Author(s):  
Ryo Shintate ◽  
Takuro Ishii ◽  
Joongho Ahn ◽  
Jin Young Kim ◽  
Chulhong Kim ◽  
...  
Keyword(s):  
High Speed ◽  
Optical Resolution ◽  
Calibration Method ◽  
Correction Method ◽  
Distortion Correction ◽  
Photoacoustic Microscopy ◽  
Imaging Tool ◽  
Imaging Sequence ◽  
Biological Interpretation

Abstract Optical resolution photoacoustic microscopy (OR-PAM) is a remarkable biomedical imaging tool that can selectively visualize microtissues with optical-dependent high resolution. However, traditional OR-PAM using mechanical stages provides slow imaging speed, making biological interpretation of in-vivo tissue difficult. Here, we developed a high-speed OR-PAM using a recently commercialized MEMS mirror. This system (MEMS-OR-PAM) consisted of a 1-axis MEMS mirror and a mechanical stage. Furthermore, this study proposed a novel calibration method that quickly removes the spatial distortion caused by fast MEMS scanning. The proposed calibration method needs to run imaging sequence only once using a ruler target and it can easily correct distortions caused by both the scan geometry of the MEMS mirror and its nonlinear motion. The combination of the MEMS-OR-PAM and the distortion correction method was verified by three experiments.; 1) Leaf skeleton phantom imaging to test the distortion correction efficacy.; 2) Spatial resolution and depth of focus (DOF) measurement for the system performance.; 3) In-Vivo finger capillaries imaging to verify their biomedical use. The results showed that the combination could achieve a high-speed (32 sec in 2 mm×4 mm) and high-lateral resolution (~6 µm) imaging capability and precisely visualize the circulating structure of the finger capillaries.

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