scholarly journals Porous silicon microparticles as an alternative support for solid phase DNA synthesis

2005 ◽  
Author(s):  
Steven McInnes ◽  
Sean Graney ◽  
Yit-lung Khung ◽  
Nicolas H. Voelcker
Keyword(s):  
Porous Silicon ◽  
Dna Synthesis ◽  
Solid Phase

Probing Optical Transitions in Porous Silicon by Reflectance Spectroscopy in the Near Infrared, Visible and UV

1994 ◽  
Vol 358 ◽  
Author(s):  
W. Theiβ ◽  
R. Arens-Fischer ◽  
M. Arntzen ◽  
M.G. Berger ◽  
S. Frohnhoff ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Dielectric Function ◽  
Near Infrared ◽  
Solid Phase ◽  
Pore Wall ◽  
Reflectance Spectroscopy ◽  
Wall Material ◽  
Bulk Silicon ◽  
Band Structure Calculations ◽  
Structure Calculations

ABSTRACTReflectance spectroscopy has been used to obtain the dielectric function of the solid phase of porous silicon. The method is based on a fit of a parameterized dielectric function model to measured spectra. A crucial step in the procedure is the 'dielectric averaging' of the microscopic dielectric function of the pore wall material to the macroscopic effective dielectric function which governs the optical properties.Results are given for heavily and moderately p-doped samples of various porosities. For the latter large differences to bulk silicon have been found. The obtained dielectric functions are compared to the results of band structure calculations taken from literature.

Automated Solid-Phase DNA Synthesis and Photophysical Properties of Oligonucleotides Labeled at the 5‘-Terminus with Ru(bpy)32+

1999 ◽  
Vol 38 (17) ◽  
pp. 3922-3925 ◽  
Author(s):  
Shoeb I. Khan ◽  
Amy E. Beilstein ◽  
Milan Sykora ◽  
Gregory D. Smith ◽  
Xi Hu ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Solid Phase ◽  
Photophysical Properties

Study on Solid Phase Crystallization of Silicon Films Deposited on Different Substrates

2011 ◽  
Vol 287-290 ◽  
pp. 1352-1355
Author(s):  
Qing Dong Chen ◽  
Jun Ping Wang ◽  
Yu Xiang Zhang
Keyword(s):  
Porous Silicon ◽  
Silicon Substrate ◽  
Solid Phase ◽  
Quartz Substrate ◽  
Silicon Crystal ◽  
Electrochemical Corrosion ◽  
Silicon Films ◽  
Solid Phase Crystallization ◽  
Boron Doped ◽  
Before And After

Porous silicon were prepared by electrochemical corrosion. Undoped and boron doped silicon films were deposited on quartz substrate、porous silicon and silicon substrate by PECVD,and were solid phase crystallized at different temperature and different hours. The microstructure of films before and after annealing were studied by Raman and XRD. The results show that:the crystallization of films deposited on porous silicon and monocrystalline silicon substrate are better than quartz substrate; The substrate which has silicon crystal lattice play an important role of seed crystal in the solid phase crystallization, the same grain orientation film can be grown on certain condition.

scholarly journals Synthesis and characterization of an antihuman T-lymphocyte saporin immunotoxin (OKT1-SAP) with in vivo stability into nonhuman primates

Blood ◽  
1988 ◽  
Vol 72 (2) ◽  
pp. 756-765 ◽  
Author(s):  
S Siena ◽  
DA Lappi ◽  
M Bregni ◽  
A Formosa ◽  
S Villa ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Dna Synthesis ◽  
T Lymphocyte ◽  
Whole Blood ◽  
Solid Phase ◽  
Plasma Concentrations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Target Cells ◽  
Blood Components

Abstract The authors conjugated, by a disulphide bond, the antihuman T- lymphocyte (CD5) monoclonal antibody (MoAb) OKT1 to the saporin-6 (SAP) ribosome-inactivating protein of the plant Saponaria officinalis. The resulting OKT1-SAP immunotoxin bound to CD5-expressing target cells and under standard culture conditions specifically suppressed mitogen- induced-T-lymphocyte DNA and protein synthesis in a dose-related manner. T-lymphocyte killing was achieved by five-minute exposure of the target cells to OKT1-SAP. The concentration inhibiting 50% (IC50) of T-lymphocyte DNA synthesis was 0.32 nmol/L. The potency of OKT1-SAP was moderately enhanced by amantadine (IC50 0.08 nmol/L) but not by ammonium chloride or chloroquine. Whole blood components did not interfere with the efficacy of OKT1-SAP, as in vitro treatment of fresh whole blood resulted in effective elimination of clonable peripheral blood T-lymphocytes assessed by a limiting dilution assay. Because these characteristics of T-lymphocyte killing by OKT1-SAP (ie, rapidity of action, potency also without potentiators) and lack of inhibition by whole blood components may be relevant for the use of an immunotoxin as a therapeutic agent in humans, the authors evaluated the stability in vivo and the circulatory clearance of OKT1-SAP in cynomolgus monkeys. Following a single intravenous (IV) injection of nontoxic dosages (0.16 to 1.3 mg/kg), an initial rapid decline (t1/2 alpha = 1.0 to 4.1 hours) was followed by a long-lasting slower decrease (t1/2 beta = 11.6 to 20.6 hours) of OKT1-SAP plasma concentrations as detected by double- antibody solid phase enzyme-linked immunosorbent assay (ELISA) assay. Not only did OKT1-SAP remain intact immunologically but it also retained its biological activity, as measured by the ability of plasma samples from monkeys given immunotoxin to inhibit DNA synthesis in human T-lymphocytes. Taken together the findings presented in this article indicate the feasibility of using OKT1-SAP as a therapeutic tool and provide information that will facilitate the rational use of immunotoxins as a treatment modality in humans.

Noncovalent Antibody Immobilization on Porous Silicon Combined with Miniaturized Solid-Phase Extraction (SPE) for Array Based ImmunoMALDI Assays

2011 ◽  
Vol 83 (12) ◽  
pp. 4942-4948 ◽  
Author(s):  
Hong Yan ◽  
Asilah Ahmad-Tajudin ◽  
Martin Bengtsson ◽  
Shoujun Xiao ◽  
Thomas Laurell ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Antibody Immobilization

A Porous Microfluidic Chip for Protein Extraction Based on Solid Phase Extraction Method

2011 ◽  
Vol 483 ◽  
pp. 297-300 ◽  
Author(s):  
Xing Chen ◽  
Da Fu Cui ◽  
Lu Lu Zhang ◽  
Hui Li ◽  
Jian Hai Sun ◽  
...  
Keyword(s):  
Surface Modification ◽  
Porous Silicon ◽  
Solid Phase Extraction ◽  
Microfluidic Chip ◽  
Protein Extraction ◽  
Solid Phase ◽  
Electrochemical Etching ◽  
Rectangular Channel ◽  
Porous Matrix ◽  
Phase Extraction

Based on the principle of solid phase extraction (SPE) and the special immunoreaction, a microfluidic chip integrated with porous matrix was developed for protein extraction. Porous matrix was achieved by electrochemical etching silicon in a HF/ethanol mixture, which was coated on the wall of the rectangular channel of the microchip to provide a surface-enlarging matrix. The surface morphology of the bare porous silicon and the porous silicon modified with the protein has been characterized by SEM. Non-porous chip and porous chip were used to extract protein. Compared with non-porous matrix, the porous matrix achieved higher extracted efficiency of protein. Then two methods of surface modification were employed on porous matrix for protein extraction. The surface modification with Protein A could extract more protein with less non-special absorption. Evaluation of the structure of the solid phase matrix and the surface modification process in the microfluidic chip, the porous microfluidic chip is able to be suitable for incorporation into micro total analysis system (μTAS).

scholarly journals Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries

2018 ◽  
Vol 14 ◽  
pp. 397-406 ◽  
Author(s):  
Ruth Suchsland ◽  
Bettina Appel ◽  
Sabine Müller
Keyword(s):  
Protein Engineering ◽  
Dna Synthesis ◽  
Solid Phase ◽  
Gene Synthesis ◽  
Gene Library ◽  
Library Preparation ◽  
Soluble Polymers ◽  
Gene Libraries ◽  
Recent Developments ◽  
Protein Libraries

The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library. The challenge in gene library preparation is to achieve controlled total or partial randomization at any predefined number and position of codons of a given gene, in order to obtain a library with a maximum number of potentially successful candidates. This purpose is best achieved by the usage of trinucleotide synthons for codon-based gene synthesis. We here review the strategies for the preparation of fully protected trinucleotides, emphasizing more recent developments for their synthesis on solid phase and on soluble polymers, and their use as synthons in standard DNA synthesis.

scholarly journals Synthesis and characterization of an antihuman T-lymphocyte saporin immunotoxin (OKT1-SAP) with in vivo stability into nonhuman primates

Blood ◽  
1988 ◽  
Vol 72 (2) ◽  
pp. 756-765
Author(s):  
S Siena ◽  
DA Lappi ◽  
M Bregni ◽  
A Formosa ◽  
S Villa ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Dna Synthesis ◽  
T Lymphocyte ◽  
Whole Blood ◽  
Solid Phase ◽  
Plasma Concentrations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Target Cells ◽  
Blood Components

The authors conjugated, by a disulphide bond, the antihuman T- lymphocyte (CD5) monoclonal antibody (MoAb) OKT1 to the saporin-6 (SAP) ribosome-inactivating protein of the plant Saponaria officinalis. The resulting OKT1-SAP immunotoxin bound to CD5-expressing target cells and under standard culture conditions specifically suppressed mitogen- induced-T-lymphocyte DNA and protein synthesis in a dose-related manner. T-lymphocyte killing was achieved by five-minute exposure of the target cells to OKT1-SAP. The concentration inhibiting 50% (IC50) of T-lymphocyte DNA synthesis was 0.32 nmol/L. The potency of OKT1-SAP was moderately enhanced by amantadine (IC50 0.08 nmol/L) but not by ammonium chloride or chloroquine. Whole blood components did not interfere with the efficacy of OKT1-SAP, as in vitro treatment of fresh whole blood resulted in effective elimination of clonable peripheral blood T-lymphocytes assessed by a limiting dilution assay. Because these characteristics of T-lymphocyte killing by OKT1-SAP (ie, rapidity of action, potency also without potentiators) and lack of inhibition by whole blood components may be relevant for the use of an immunotoxin as a therapeutic agent in humans, the authors evaluated the stability in vivo and the circulatory clearance of OKT1-SAP in cynomolgus monkeys. Following a single intravenous (IV) injection of nontoxic dosages (0.16 to 1.3 mg/kg), an initial rapid decline (t1/2 alpha = 1.0 to 4.1 hours) was followed by a long-lasting slower decrease (t1/2 beta = 11.6 to 20.6 hours) of OKT1-SAP plasma concentrations as detected by double- antibody solid phase enzyme-linked immunosorbent assay (ELISA) assay. Not only did OKT1-SAP remain intact immunologically but it also retained its biological activity, as measured by the ability of plasma samples from monkeys given immunotoxin to inhibit DNA synthesis in human T-lymphocytes. Taken together the findings presented in this article indicate the feasibility of using OKT1-SAP as a therapeutic tool and provide information that will facilitate the rational use of immunotoxins as a treatment modality in humans.

Stabilization of hydroxyl-terminated silanes in porous silicon for in-situ DNA synthesis

2010 ◽  
Vol 8 (6) ◽  
pp. 1851-1855 ◽  
Author(s):  
Jenifer L. Lawrie ◽  
Sharon M. Weiss
Keyword(s):  
Porous Silicon ◽  
Dna Synthesis
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