Noncovalent Antibody Immobilization on Porous Silicon Combined with Miniaturized Solid-Phase Extraction (SPE) for Array Based ImmunoMALDI Assays

2011 ◽  
Vol 83 (12) ◽  
pp. 4942-4948 ◽  
Author(s):  
Hong Yan ◽  
Asilah Ahmad-Tajudin ◽  
Martin Bengtsson ◽  
Shoujun Xiao ◽  
Thomas Laurell ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Antibody Immobilization

A Porous Microfluidic Chip for Protein Extraction Based on Solid Phase Extraction Method

2011 ◽  
Vol 483 ◽  
pp. 297-300 ◽  
Author(s):  
Xing Chen ◽  
Da Fu Cui ◽  
Lu Lu Zhang ◽  
Hui Li ◽  
Jian Hai Sun ◽  
...  
Keyword(s):  
Surface Modification ◽  
Porous Silicon ◽  
Solid Phase Extraction ◽  
Microfluidic Chip ◽  
Protein Extraction ◽  
Solid Phase ◽  
Electrochemical Etching ◽  
Rectangular Channel ◽  
Porous Matrix ◽  
Phase Extraction

Based on the principle of solid phase extraction (SPE) and the special immunoreaction, a microfluidic chip integrated with porous matrix was developed for protein extraction. Porous matrix was achieved by electrochemical etching silicon in a HF/ethanol mixture, which was coated on the wall of the rectangular channel of the microchip to provide a surface-enlarging matrix. The surface morphology of the bare porous silicon and the porous silicon modified with the protein has been characterized by SEM. Non-porous chip and porous chip were used to extract protein. Compared with non-porous matrix, the porous matrix achieved higher extracted efficiency of protein. Then two methods of surface modification were employed on porous matrix for protein extraction. The surface modification with Protein A could extract more protein with less non-special absorption. Evaluation of the structure of the solid phase matrix and the surface modification process in the microfluidic chip, the porous microfluidic chip is able to be suitable for incorporation into micro total analysis system (μTAS).

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