Automated Solid-Phase DNA Synthesis and Photophysical Properties of Oligonucleotides Labeled at the 5‘-Terminus with Ru(bpy)32+

1999 ◽  
Vol 38 (17) ◽  
pp. 3922-3925 ◽  
Author(s):  
Shoeb I. Khan ◽  
Amy E. Beilstein ◽  
Milan Sykora ◽  
Gregory D. Smith ◽  
Xi Hu ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Solid Phase ◽  
Photophysical Properties

Solid-Phase Synthesis and Photophysical Properties of DNA Labeled at the Nucleobase with Ru(bpy)2(4-m-4‘-pa-bpy)2+

1999 ◽  
Vol 38 (26) ◽  
pp. 5999-6002 ◽  
Author(s):  
Shoeb I. Khan ◽  
Amy E. Beilstein ◽  
Mark T. Tierney ◽  
Milan Sykora ◽  
Mark W. Grinstaff
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Photophysical Properties ◽  
Phase Synthesis

Automated Solid-Phase Synthesis and Photophysical Properties of Oligodeoxynucleotides Labeled at 5‘-Aminothymidine with Ru(bpy)2(4-m-4‘-cam-bpy)2+

2000 ◽  
Vol 39 (12) ◽  
pp. 2500-2504 ◽  
Author(s):  
Xi Hu ◽  
Gregory D. Smith ◽  
Milan Sykora ◽  
Stephen J. Lee ◽  
Mark W. Grinstaff
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Photophysical Properties ◽  
Phase Synthesis

scholarly journals Macromolecular Dyes by Chromophore-Initiated Ring Opening Polymerization of L-Lactide

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1979
Author(s):  
Francesca Cicogna ◽  
Guido Giachi ◽  
Luca Rosi ◽  
Elisa Passaglia ◽  
Serena Coiai ◽  
...  
Keyword(s):  
Ring Opening ◽  
Solid Phase ◽  
Photophysical Properties ◽  
Fluorescence Emission ◽  
Ring Opening Polymerization ◽  
Polymerization Process ◽  
Polymerization Reaction ◽  
Poly Lactic Acid ◽  
Electromagnetic Spectrum ◽  
Visible Part

End functionalized polylactides are prepared by ring opening polymerization of L-lactide in the presence of stannous octoate (Sn(Oct)2). Three chromophores, 9H-carbazol-ethanol (CA), 9-fluorenyl-methanol (FM), and 2-(4-(2-chloro-4-nitrophenylazo)-N-ethylphenylamino)ethanol (Disperse Red 13, DR), are for the first time used as co-initiators in the polymerization process. The polymerization reaction is initiated by conventional thermal treatment, but in the case of FM, microwave-assisted polymerization is also carried out. CA and FM absorb and emit in the UV portion of the electromagnetic spectrum, whereas DR absorbs in the visible part. The obtained end-capped polylactides derivatives show the same photophysical properties as the initiator, so they are “macromolecular dyes” (MDs) that can be used “as synthesized” or can be blended with commercial poly(lactic acid) (PLA). The blends of PLA with MDs have ultraviolet-visible (UV-Vis) absorption and fluorescence emission features similar to that of MDs and thermal properties typical of PLA. Finally, migration tests, carried out onto the blends of PLA with MDs and PLA with free chromophores, show that MDs are less released than free chromophores both in solution and in the solid phase.

scholarly journals Synthesis and characterization of an antihuman T-lymphocyte saporin immunotoxin (OKT1-SAP) with in vivo stability into nonhuman primates

Blood ◽  
1988 ◽  
Vol 72 (2) ◽  
pp. 756-765 ◽  
Author(s):  
S Siena ◽  
DA Lappi ◽  
M Bregni ◽  
A Formosa ◽  
S Villa ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Dna Synthesis ◽  
T Lymphocyte ◽  
Whole Blood ◽  
Solid Phase ◽  
Plasma Concentrations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Target Cells ◽  
Blood Components

Abstract The authors conjugated, by a disulphide bond, the antihuman T- lymphocyte (CD5) monoclonal antibody (MoAb) OKT1 to the saporin-6 (SAP) ribosome-inactivating protein of the plant Saponaria officinalis. The resulting OKT1-SAP immunotoxin bound to CD5-expressing target cells and under standard culture conditions specifically suppressed mitogen- induced-T-lymphocyte DNA and protein synthesis in a dose-related manner. T-lymphocyte killing was achieved by five-minute exposure of the target cells to OKT1-SAP. The concentration inhibiting 50% (IC50) of T-lymphocyte DNA synthesis was 0.32 nmol/L. The potency of OKT1-SAP was moderately enhanced by amantadine (IC50 0.08 nmol/L) but not by ammonium chloride or chloroquine. Whole blood components did not interfere with the efficacy of OKT1-SAP, as in vitro treatment of fresh whole blood resulted in effective elimination of clonable peripheral blood T-lymphocytes assessed by a limiting dilution assay. Because these characteristics of T-lymphocyte killing by OKT1-SAP (ie, rapidity of action, potency also without potentiators) and lack of inhibition by whole blood components may be relevant for the use of an immunotoxin as a therapeutic agent in humans, the authors evaluated the stability in vivo and the circulatory clearance of OKT1-SAP in cynomolgus monkeys. Following a single intravenous (IV) injection of nontoxic dosages (0.16 to 1.3 mg/kg), an initial rapid decline (t1/2 alpha = 1.0 to 4.1 hours) was followed by a long-lasting slower decrease (t1/2 beta = 11.6 to 20.6 hours) of OKT1-SAP plasma concentrations as detected by double- antibody solid phase enzyme-linked immunosorbent assay (ELISA) assay. Not only did OKT1-SAP remain intact immunologically but it also retained its biological activity, as measured by the ability of plasma samples from monkeys given immunotoxin to inhibit DNA synthesis in human T-lymphocytes. Taken together the findings presented in this article indicate the feasibility of using OKT1-SAP as a therapeutic tool and provide information that will facilitate the rational use of immunotoxins as a treatment modality in humans.

scholarly journals Porous silicon microparticles as an alternative support for solid phase DNA synthesis

2005 ◽  
Author(s):  
Steven McInnes ◽  
Sean Graney ◽  
Yit-lung Khung ◽  
Nicolas H. Voelcker
Keyword(s):  
Porous Silicon ◽  
Dna Synthesis ◽  
Solid Phase

scholarly journals Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries

2018 ◽  
Vol 14 ◽  
pp. 397-406 ◽  
Author(s):  
Ruth Suchsland ◽  
Bettina Appel ◽  
Sabine Müller
Keyword(s):  
Protein Engineering ◽  
Dna Synthesis ◽  
Solid Phase ◽  
Gene Synthesis ◽  
Gene Library ◽  
Library Preparation ◽  
Soluble Polymers ◽  
Gene Libraries ◽  
Recent Developments ◽  
Protein Libraries

The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library. The challenge in gene library preparation is to achieve controlled total or partial randomization at any predefined number and position of codons of a given gene, in order to obtain a library with a maximum number of potentially successful candidates. This purpose is best achieved by the usage of trinucleotide synthons for codon-based gene synthesis. We here review the strategies for the preparation of fully protected trinucleotides, emphasizing more recent developments for their synthesis on solid phase and on soluble polymers, and their use as synthons in standard DNA synthesis.

scholarly journals Synthesis and characterization of an antihuman T-lymphocyte saporin immunotoxin (OKT1-SAP) with in vivo stability into nonhuman primates

Blood ◽  
1988 ◽  
Vol 72 (2) ◽  
pp. 756-765
Author(s):  
S Siena ◽  
DA Lappi ◽  
M Bregni ◽  
A Formosa ◽  
S Villa ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Dna Synthesis ◽  
T Lymphocyte ◽  
Whole Blood ◽  
Solid Phase ◽  
Plasma Concentrations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Target Cells ◽  
Blood Components

The authors conjugated, by a disulphide bond, the antihuman T- lymphocyte (CD5) monoclonal antibody (MoAb) OKT1 to the saporin-6 (SAP) ribosome-inactivating protein of the plant Saponaria officinalis. The resulting OKT1-SAP immunotoxin bound to CD5-expressing target cells and under standard culture conditions specifically suppressed mitogen- induced-T-lymphocyte DNA and protein synthesis in a dose-related manner. T-lymphocyte killing was achieved by five-minute exposure of the target cells to OKT1-SAP. The concentration inhibiting 50% (IC50) of T-lymphocyte DNA synthesis was 0.32 nmol/L. The potency of OKT1-SAP was moderately enhanced by amantadine (IC50 0.08 nmol/L) but not by ammonium chloride or chloroquine. Whole blood components did not interfere with the efficacy of OKT1-SAP, as in vitro treatment of fresh whole blood resulted in effective elimination of clonable peripheral blood T-lymphocytes assessed by a limiting dilution assay. Because these characteristics of T-lymphocyte killing by OKT1-SAP (ie, rapidity of action, potency also without potentiators) and lack of inhibition by whole blood components may be relevant for the use of an immunotoxin as a therapeutic agent in humans, the authors evaluated the stability in vivo and the circulatory clearance of OKT1-SAP in cynomolgus monkeys. Following a single intravenous (IV) injection of nontoxic dosages (0.16 to 1.3 mg/kg), an initial rapid decline (t1/2 alpha = 1.0 to 4.1 hours) was followed by a long-lasting slower decrease (t1/2 beta = 11.6 to 20.6 hours) of OKT1-SAP plasma concentrations as detected by double- antibody solid phase enzyme-linked immunosorbent assay (ELISA) assay. Not only did OKT1-SAP remain intact immunologically but it also retained its biological activity, as measured by the ability of plasma samples from monkeys given immunotoxin to inhibit DNA synthesis in human T-lymphocytes. Taken together the findings presented in this article indicate the feasibility of using OKT1-SAP as a therapeutic tool and provide information that will facilitate the rational use of immunotoxins as a treatment modality in humans.

scholarly journals A New Universal Linker for Solid Phase DNA Synthesis

1996 ◽  
Vol 24 (14) ◽  
pp. 2793-2798 ◽  
Author(s):  
M. H. Lyttle ◽  
D. Hudson ◽  
R. M. Cook
Keyword(s):  
Dna Synthesis ◽  
Solid Phase

scholarly journals Photophysical properties of thin films and solid phase of switchable supermolecular anthracene-based rotaxanes

2001 ◽  
Vol 122 (1) ◽  
pp. 63-65 ◽  
Author(s):  
G. Giro ◽  
M. Cocchi ◽  
V. Fattori ◽  
G. Gadret ◽  
G. Ruani ◽  
...  
Keyword(s):  
Thin Films ◽  
Solid Phase ◽  
Photophysical Properties

SEMICONDUCTOR NANOPARTICLES AS REPORTERS IN MULTIPLEXED IMMUNOASSAY AND CELL ANALYSIS

2009 ◽  
Vol 08 (01n02) ◽  
pp. 163-167 ◽  
Author(s):  
TIM DUBROVSKY
Keyword(s):  
Quantum Dots ◽  
Research And Development ◽  
Spectral Properties ◽  
Solid Phase ◽  
Photophysical Properties ◽  
Cell Analysis ◽  
Biological Applications ◽  
Solid Phase Immunoassay ◽  
Multiplexed Immunoassay ◽  
Stokes Shifts

The development of new fluorophores has experienced a tremendous advance over the last two decades. Here, we discuss the photophysical properties of traditional fluorescent labels and the comparatively advantageous properties of quantum dots. The unique spectral properties of quantum dots, such as their large Stokes shifts and exceptional brightness, make them attractive probes in biological applications and prime candidates for further research and development in the field of solid-phase immunoassay and cell analysis.

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