High-speed position and force measurements in optical tweezers (Conference Presentation)

2017 ◽  
Author(s):  
Anatolii V. Kashchuk ◽  
Alexander B. Stilgoe ◽  
Timo A. Nieminen ◽  
Halina H. Rubinsztein-Dunlop
Keyword(s):  
Optical Tweezers ◽  
High Speed ◽  
Force Measurements

scholarly journals Hemodynamic forces can be accurately measured in vivo with optical tweezers

2017 ◽  
Author(s):  
Sébastien Harlepp ◽  
Fabrice Thalmann ◽  
Gautier Follain ◽  
Jacky G. Goetz
Keyword(s):  
Optical Tweezers ◽  
Cell Biology ◽  
Accurate Determination ◽  
Force Measurements ◽  
Hemodynamic Forces ◽  
Non Invasive ◽  
Drag Forces ◽  
Measured Flow ◽  
Living Organisms

AbstractForce sensing and generation at the tissular and cellular scale is central to many biological events. There is a growing interest in modern cell biology for methods enabling force measurements in vivo. Optical trapping allows non-invasive probing of pico-Newton forces and thus emerged as a promising mean for assessing biomechanics in vivo. Nevertheless, the main obstacles rely in the accurate determination of the trap stiffness in heterogeneous living organisms, at any position where the trap is used. A proper calibration of the trap stiffness is thus required for performing accurate and reliable force measurements in vivo. Here, we introduce a method that overcomes these difficulties by accurately measuring hemodynamic profiles in order to calibrate the trap stiffness. Doing so, and using numerical methods to assess the accuracy of the experimental data, we measured flow profiles and drag forces imposed to trapped red blood cells of living zebrafish embryos. Using treatments enabling blood flow tuning, we demonstrated that such method is powerful in measuring hemodynamic forces in vivo with accuracy and confidence. Altogether, this study demonstrates the power of optical tweezing in measuring low range hemodynamic forces in vivo and offers an unprecedented tool in both cell and developmental biology.

Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

2001 ◽  
Author(s):  
Bahman Anvari ◽  
Zhiwei Li ◽  
Masayoshi Takashima ◽  
Peter Brecht ◽  
Jorge H. Torres ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Optical Tweezers ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
Force Measurements

scholarly journals Optical Tweezers for Quantitative Force Measurements and Live Cell Experiments

2014 ◽  
Vol 22 (4) ◽  
pp. 24-31 ◽  
Author(s):  
Philipp Rauch ◽  
Torsten Jähnke
Keyword(s):  
Optical Tweezers ◽  
Live Cell ◽  
Force Measurements

Precision force measurements with optical tweezers

2020 ◽  
Author(s):  
Declan Armstrong ◽  
Alexander B. Stilgoe ◽  
Timo Nieminen ◽  
Halina Rubinsztein-Dunlop
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

scholarly journals Functional morphology of suction discs and attachment performance of the Mediterranean medicinal leech ( Hirudo verbana Carena)

2016 ◽  
Vol 13 (117) ◽  
pp. 20160096 ◽  
Author(s):  
Tim Kampowski ◽  
Laura Eberhard ◽  
Friederike Gallenmüller ◽  
Thomas Speck ◽  
Simon Poppinga
Keyword(s):  
Functional Morphology ◽  
High Speed ◽  
Substrate Surface ◽  
Surrounding Medium ◽  
Medicinal Leech ◽  
Force Measurements ◽  
High Speed Video ◽  
Hirudo Verbana ◽  
Substrate Surface Roughness ◽  
Medicinal Leeches

Medicinal leeches use their suction discs for locomotion, adhesion to the host and, in the case of the anterior disc, also for blood ingestion. The biomechanics of their suction-based adhesion systems has been little understood until now. We investigated the functional morphology of the anterior and posterior suckers of Hirudo verbana by using light and scanning electron microscopy. Furthermore, we analysed the adhesion qualitatively and quantitatively by conducting behavioural and mechanical experiments. Our high-speed video analyses provide new insights into the attachment and detachment processes and we present a detailed description of the leech locomotion cycle. Pull-off force measurements of the anterior and posterior suction organs on seven different substrates under both aerial and water-submersed conditions reveal a significant influence of the surrounding medium, the substrate surface roughness and the tested organ on attachment forces and tenacities.

Optical tweezers force measurements to study parasites chemotaxis

2009 ◽  
Author(s):  
A. A. de Thomaz ◽  
L. Y. Pozzo ◽  
A. Fontes ◽  
D. B. Almeida ◽  
C. V. Stahl ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

scholarly journals Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy

2008 ◽  
Vol 16 (19) ◽  
pp. 14561 ◽  
Author(s):  
Graham M. Gibson ◽  
Jonathan Leach ◽  
Stephen Keen ◽  
Amanda J. Wright ◽  
Miles J. Padgett
Keyword(s):  
Optical Tweezers ◽  
High Speed ◽  
Video Microscopy ◽  
Particle Position ◽  
High Speed Video
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