Optical tweezers force measurements to study parasites chemotaxis

2009 ◽  
Author(s):  
A. A. de Thomaz ◽  
L. Y. Pozzo ◽  
A. Fontes ◽  
D. B. Almeida ◽  
C. V. Stahl ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

scholarly journals Hemodynamic forces can be accurately measured in vivo with optical tweezers

2017 ◽  
Author(s):  
Sébastien Harlepp ◽  
Fabrice Thalmann ◽  
Gautier Follain ◽  
Jacky G. Goetz
Keyword(s):  
Optical Tweezers ◽  
Cell Biology ◽  
Accurate Determination ◽  
Force Measurements ◽  
Hemodynamic Forces ◽  
Non Invasive ◽  
Drag Forces ◽  
Measured Flow ◽  
Living Organisms

AbstractForce sensing and generation at the tissular and cellular scale is central to many biological events. There is a growing interest in modern cell biology for methods enabling force measurements in vivo. Optical trapping allows non-invasive probing of pico-Newton forces and thus emerged as a promising mean for assessing biomechanics in vivo. Nevertheless, the main obstacles rely in the accurate determination of the trap stiffness in heterogeneous living organisms, at any position where the trap is used. A proper calibration of the trap stiffness is thus required for performing accurate and reliable force measurements in vivo. Here, we introduce a method that overcomes these difficulties by accurately measuring hemodynamic profiles in order to calibrate the trap stiffness. Doing so, and using numerical methods to assess the accuracy of the experimental data, we measured flow profiles and drag forces imposed to trapped red blood cells of living zebrafish embryos. Using treatments enabling blood flow tuning, we demonstrated that such method is powerful in measuring hemodynamic forces in vivo with accuracy and confidence. Altogether, this study demonstrates the power of optical tweezing in measuring low range hemodynamic forces in vivo and offers an unprecedented tool in both cell and developmental biology.

Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

2001 ◽  
Author(s):  
Bahman Anvari ◽  
Zhiwei Li ◽  
Masayoshi Takashima ◽  
Peter Brecht ◽  
Jorge H. Torres ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Optical Tweezers ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
Force Measurements

scholarly journals Optical Tweezers for Quantitative Force Measurements and Live Cell Experiments

2014 ◽  
Vol 22 (4) ◽  
pp. 24-31 ◽  
Author(s):  
Philipp Rauch ◽  
Torsten Jähnke
Keyword(s):  
Optical Tweezers ◽  
Live Cell ◽  
Force Measurements

Precision force measurements with optical tweezers

2020 ◽  
Author(s):  
Declan Armstrong ◽  
Alexander B. Stilgoe ◽  
Timo Nieminen ◽  
Halina Rubinsztein-Dunlop
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

scholarly journals Calibration of Optical Tweezers for In Vivo Force Measurements: How do Different Approaches Compare?

2014 ◽  
Vol 107 (6) ◽  
pp. 1474-1484 ◽  
Author(s):  
Yonggun Jun ◽  
Suvranta K. Tripathy ◽  
Babu R.J. Narayanareddy ◽  
Michelle K. Mattson-Hoss ◽  
Steven P. Gross
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

Displacement and Force Measurements with Quadrant Photodetector in Optical Tweezers

2003 ◽  
Vol 20 (6) ◽  
pp. 950-952 ◽  
Author(s):  
Guo Hong-Lian ◽  
Liu Chun-Xiang ◽  
Li Zhao-Lin ◽  
Duan Jian-Fa ◽  
Han Xue-Hai ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Force Measurements ◽  
Quadrant Photodetector

scholarly journals Probing Ligand-Receptor Interaction in Living Cells Using Force Measurements With Optical Tweezers

2020 ◽  
Vol 8 ◽  
Author(s):  
Carolin Riesenberg ◽  
Christian Alejandro Iriarte-Valdez ◽  
Annegret Becker ◽  
Maria Dienerowitz ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Living Cells ◽  
Receptor Interaction ◽  
Force Measurements
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