scholarly journals Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy

2008 ◽  
Vol 16 (19) ◽  
pp. 14561 ◽  
Author(s):  
Graham M. Gibson ◽  
Jonathan Leach ◽  
Stephen Keen ◽  
Amanda J. Wright ◽  
Miles J. Padgett
Keyword(s):  
Optical Tweezers ◽  
High Speed ◽  
Video Microscopy ◽  
Particle Position ◽  
High Speed Video

scholarly journals V01-04 HIGH-SPEED VIDEO MICROSCOPY INVESTIGATIONS OF THE MECHANISMS OF STONE BREAKAGE WITH HOLMIUM:YAG LASER LITHOTRIPSY

2019 ◽  
Vol 201 (Supplement 4) ◽  
Author(s):  
Yuri Pishchalnikov* ◽  
William Behnke-Parks ◽  
Daniel Laser ◽  
Marshall Stoller
Keyword(s):  
High Speed ◽  
Laser Lithotripsy ◽  
Video Microscopy ◽  
Holmium:Yag Laser ◽  
High Speed Video

scholarly journals Intracellular Cl− Regulation of Ciliary Beating in Ciliated Human Nasal Epithelial Cells: Frequency and Distance of Ciliary Beating Observed by High-Speed Video Microscopy

2020 ◽  
Vol 21 (11) ◽  
pp. 4052
Author(s):  
Makoto Yasuda ◽  
Taka-aki Inui ◽  
Shigeru Hirano ◽  
Shinji Asano ◽  
Tomonori Okazaki ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
High Speed ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Video Microscopy ◽  
Nasal Epithelial Cells ◽  
Ciliary Beating ◽  
High Speed Video ◽  
Human Nasal Epithelial Cells ◽  
Ciliary Beat

Small inhaled particles, which are entrapped by the mucous layer that is maintained by mucous secretion via mucin exocytosis and fluid secretion, are removed from the nasal cavity by beating cilia. The functional activities of beating cilia are assessed by their frequency and the amplitude. Nasal ciliary beating is controlled by intracellular ions (Ca2+, H+ and Cl−), and is enhanced by a decreased concentration of intracellular Cl− ([Cl−]i) in ciliated human nasal epithelial cells (cHNECs) in primary culture, which increases the ciliary beat amplitude. A novel method to measure both ciliary beat frequency (CBF) and ciliary beat distance (CBD, an index of ciliary beat amplitude) in cHNECs has been developed using high-speed video microscopy, which revealed that a decrease in [Cl−]i increased CBD, but not CBF, and an increase in [Cl−]i decreased both CBD and CBF. Thus, [Cl−]i inhibits ciliary beating in cHNECs, suggesting that axonemal structures controlling CBD and CBF may have Cl− sensors and be regulated by [Cl−]i. These observations indicate that the activation of Cl− secretion stimulates ciliary beating (increased CBD) mediated via a decrease in [Cl−]i in cHNECs. Thus, [Cl−]i is critical for controlling ciliary beating in cHNECs. This review introduces the concept of Cl− regulation of ciliary beating in cHNECs.

Laboratory experiments on secondary ice production upon free falling drizzle droplets observed by high speed video and thermal imaging

2021 ◽  
Author(s):  
Alice Keinert ◽  
Judith Kleinheins ◽  
Alexei Kiselev ◽  
Thomas Leisner
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Video Microscopy ◽  
Freezing Process ◽  
Crack Open ◽  
High Speed Video ◽  
Pressure Release ◽  
Ice Particles ◽  
Ice Production ◽  
Ice Shell

<p>During the freezing of supercooled drizzle droplets, the ice shell forms at the droplet surface and propagates inwards, causing a pressure rise in the droplet core. If the pressure exceeds the mechanical stability of the ice shell, the shell can crack open and eject secondary ice particles or cause the full disintegration of the ice shell leading to droplet shattering. Recent in-cloud observations and modeling studies have suggested the importance of secondary ice production upon shattering of freezing drizzle droplets. The details of this process are poorly understood and the number of secondary ice particles produced during freezing remains to be quantified.</p><p>Here we present insight into experiments with freezing drizzle droplets levitated in electrodynamic balance under controlled conditions with respect to temperature, humidity and airflow velocity. Individual droplets are exposed to a flow of cold air from below, simulating free fall conditions. The freezing process is observed with high-speed video microscopy and a high-resolution infrared thermal measuring system. We show the observed frequencies for various events associated with the production of secondary ice particles during freezing for pure water droplets and aqueous solution of analogue sea salt droplets (300 µm in diameter) and report a strong enhancement of the shattering probability as compared to our previous study (Lauber et al., 2018) conducted in stagnant air. Analysis of pressure release events recorded by high-resolution infrared thermography suggest that pressure release events associated with the possible ejection of secondary ice particles occur far more frequent than previously quantified with observations by high speed video microscopy only.</p><p> </p><p>Lauber, A., A. Kiselev, T. Pander, P. Handmann, and T Leisner (2018). “Secondary Ice Formation during Freezing of Levitated Droplets”, Journal of the Atmospheric Sciences 75, pp. 2815–2826. </p>

Ciliary beat frequency study by high speed video microscopy

2016 ◽  
Author(s):  
Gerardo Vizmanos-Lamotte ◽  
Sandra Rovira ◽  
Alba Torrent ◽  
Antonio Moreno-Galdó
Keyword(s):  
High Speed ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Video Microscopy ◽  
High Speed Video ◽  
Ciliary Beat

Accuracy of high-speed video microscopy to diagnose primary ciliary dyskinesia

2018 ◽  
Author(s):  
Bruna Rubbo ◽  
Isabel Reading ◽  
Amelia Shoemark ◽  
Claire Jackson ◽  
Robert A. Hirst ◽  
...  
Keyword(s):  
Primary Ciliary Dyskinesia ◽  
High Speed ◽  
Video Microscopy ◽  
High Speed Video ◽  
Ciliary Dyskinesia

scholarly journals A How-To guide to: Quantitative high-speed video profiling to discriminate between variants of primary ciliary dyskinesia

2019 ◽  
Author(s):  
M. Chioccioli ◽  
L. Feriani ◽  
Q. Nguyen ◽  
J. Kotar ◽  
S. D. Dell ◽  
...  
Keyword(s):  
Primary Ciliary Dyskinesia ◽  
High Speed ◽  
Video Microscopy ◽  
High Speed Video ◽  
Ciliary Dyskinesia

AbstractThis document presents detailed steps to analysing the waveforms of beating cilia, measured in high speed video microscopy. We show that in the case of PCD caused by mutations inDNAH11andHYDINthe classification by a handful of parameters describing ciliary dynamics allows to distinguish the genotype, as well as (much more easily) distinguishing healthy from PCD samples. This document is intended to complement the brief highlight (1) and presents the details of the datasets used in that letter.

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