Synchronized manipulation and force measurement by optical tweezers using high-speed laser scanning

2004 ◽  
Author(s):  
F. Arai ◽  
K. Yoshikawa ◽  
T. Sakami ◽  
T. Fukuda
Keyword(s):  
Optical Tweezers ◽  
High Speed ◽  
Laser Scanning ◽  
Force Measurement

Five-Dimensional Microscopy using Widefield-Deconvolution: Practical Considerations and Biological Applications

1996 ◽  
Vol 54 ◽  
pp. 268-269
Author(s):  
W.F. Marshall ◽  
K. Oegema ◽  
J. Nunnari ◽  
A.F. Straight ◽  
D.A. Agard ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
High Speed ◽  
Laser Scanning ◽  
Ccd Camera ◽  
Image Plane ◽  
Time Lapse ◽  
Laser Scanning Confocal Microscopy ◽  
Three Dimensions ◽  
Excitation Light ◽  
Cooled Ccd

The ability to image cells in three dimensions has brought about a revolution in biological microscopy, enabling many questions to be asked which would be inaccessible without this capability. There are currently two major methods of three dimensional microscopy: laser-scanning confocal microscopy and widefield-deconvolution microscopy. The method of widefield-deconvolution uses a cooled CCD to acquire images from a standard widefield microscope, and then computationally removes out of focus blur. Using such a scheme, it is easy to acquire time-lapse 3D images of living cells without killing them, and to do so for multiple wavelengths (using computer-controlled filter wheels). Thus, it is now not only feasible, but routine, to perform five dimensional microscopy (three spatial dimensions, plus time, plus wavelength).Widefield-deconvolution has several advantages over confocal microscopy. The two main advantages are high speed of acquisition (because there is no scanning, a single optical section is acquired at a time by using a cooled CCD camera) and the use of low excitation light levels Excitation intensity can be much lower than in a confocal microscope for three reasons: 1) longer exposures can be taken since the entire 512x512 image plane is acquired in parallel, so that dwell time is not an issue, 2) the higher quantum efficiently of a CCD detect over those typically used in confocal microscopy (although this is expected to change due to advances in confocal detector technology), and 3) because no pinhole is used to reject light, a much larger fraction of the emitted light is collected. Thus we can typically acquire images with thousands of photons per pixel using a mercury lamp, instead of a laser, for illumination. The use of low excitation light is critical for living samples, and also reduces bleaching. The high speed of widefield microscopy is also essential for time-lapse 3D microscopy, since one must acquire images quickly enough to resolve interesting events.

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

scholarly journals Shock Tube as an Impulsive Application Device

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Soumya Ranjan Nanda ◽  
Sumit Agarwal ◽  
Vinayak Kulkarni ◽  
Niranjan Sahoo
Keyword(s):  
Heat Transfer ◽  
Shock Tube ◽  
High Speed ◽  
Force Measurement ◽  
Peak Load ◽  
Wave Force ◽  
Impulsive Loading ◽  
Force Balance ◽  
Test Model ◽  
Impulse Facility

Current investigations solely focus on application of an impulse facility in diverse area of high-speed aerodynamics and structural mechanics. Shock tube, the fundamental impulse facility, is specially designed and calibrated for present objectives. Force measurement experiments are performed on a hemispherical test model integrated with the stress wave force balance. Similar test model is considered for heat transfer measurements using coaxial thermocouple. Force and heat transfer experiments demonstrated that the strain gauge and thermocouple have lag time of 11.5 and 9 microseconds, respectively. Response time of these sensors in measuring the peak load is also measured successfully using shock tube facility. As an outcome, these sensors are found to be suitable for impulse testing. Lastly, the response of aluminum plates subjected to impulsive loading is analyzed by measuring the in-plane strain produced during deformation. Thus, possibility of forming tests in shock is also confirmed.

Dissolution Behavior of Cast WC Particles in NiCrBSi-WC Coatings by Laser Induction Hybrid Cladding

2013 ◽  
Vol 668 ◽  
pp. 283-287
Author(s):  
Sheng Feng Zhou ◽  
Xiao Qin Dai
Keyword(s):  
High Speed ◽  
Laser Scanning ◽  
Metallic Matrix ◽  
High Hardness ◽  
Scanning Speed ◽  
Eutectic Structure ◽  
Dissolution Behavior ◽  
Positive Role ◽  
Laser Scanning Speed ◽  
Laser Induction

In order to characterize the dissolution of cast WC particles in Ni-based WC coatings by laser induction hybrid rapid cladding, NiCrBSi+50 wt.% WC coatings are produced on A3 steel by low and high speed laser induction hybrid cladding (LIHC). When laser scanning speed is only 600 mm/min, the crack-free coating has pores and its dilution is as high as 45%. At the bottom of coating, the cast WC particles are dissolved completely and the herringbone M6C eutectics are precipitated. In the center of coating, the cast WC particles are also dissolved completely and the acicular, blocky and dendritic carbides with relatively low hardness are precipitated. At two sides of coating, some cast WC particles are dissolved partially and interact with Ni-based alloy to form an alloyed reaction layer, while others preserve the primary eutectic structure and high hardness. When laser scanning speed and powder feeding rate are increased to 1500 mm/min and 85.6 g/min, the coating has cracks but no pores. Its dilution can be markedly decreased to 7.8%. Moreover, a majority of WC particles are still composed of primary eutectic structure and keep their high hardness, which can play a positive role in strengthening Ni-based metallic matrix.

High-speed hybrid laser scanning recorder

1981 ◽  
Vol 17 (12) ◽  
pp. 2478-2480
Author(s):  
M. Graser
Keyword(s):  
High Speed ◽  
Laser Scanning

High speed laser scanning using MEMS driven in-plane vibratory grating: Design, modeling and fabrication

2009 ◽  
Vol 156 (1) ◽  
pp. 134-144 ◽  
Author(s):  
Yu Du ◽  
Guangya Zhou ◽  
Kelvin K.L. Cheo ◽  
Qingxin Zhang ◽  
Hanhua Feng ◽  
...  
Keyword(s):  
High Speed ◽  
Laser Scanning ◽  
Grating Design

scholarly journals Spray deposition inside multiple-row nursery trees with a laser-guided sprayer1

2017 ◽  
Vol 35 (1) ◽  
pp. 13-23 ◽  
Author(s):  
H. Zhu ◽  
H. Liu ◽  
Y. Shen ◽  
Hengyu Liu ◽  
R. H. Zondag
Keyword(s):  
High Speed ◽  
Laser Scanning ◽  
Quercus Rubra ◽  
Spray Deposition ◽  
Field Tests ◽  
Red Oak ◽  
Northern Red Oak ◽  
Tree Structures ◽  
Speed Sensor ◽  
Tilia Tomentosa

Abstract Multiple-row container-grown trees require specially designed sprayers to achieve efficient spray delivery. A five-port air-assisted sprayer with both automatic and manual control modes was developed to discharge adequate spray deposition inside multiple-row trees. The sprayer resulted from integration of a high-speed laser-scanning sensor with a sophisticated nozzle flow control system, an embedded computer with a touch screen, a Doppler speed sensor, a specially-designed algorithm and an air-assisted sprayer base. It was able to detect target tree presence and measure target tree size, shape and leaf density. The sprayer then controlled the spray output of each nozzle to match tree structures. The sprayer was tested for its sprayer deposition quality inside canopies in a four-row sterling silver linden (Tilia tomentosa 'Sterling Silver' Moench) field and another six-row northern red oak (Quercus rubra L.) field. Tests were conducted with the sprayer in variable-rate application (VRA) and constant-rate application (CRA) modes. The average spray deposit on foliage of trees was 1.37±0.47 μL cm−2 from VRA and 1.29±0.42 μL cm−2 from CRA in linden, and was 2.15±0.57 μL cm−2 from VRA and 2.72±0.94 μL cm−2 from CRA in red oak, respectively. In comparison, spray coverage on foliage of trees was 19.8±3.0% from VRA and 20.9±4.3% from CRA in the linden trial, and was 27.9±3.7% from VRA and 30.5±5.4% from CRA, respectively, in the red oak trial. The newly developed air-assisted sprayer in both VRA and CRA modes would be able to discharge adequate spray deposition inside multiple-row tree plants while conserving pesticide. Index words: environmental protection, pest control, pesticide, precision sprayer, spray coverage. Species used in this study: sterling silver linden (Tilia tomentosa 'Sterling Silver' Moench), northern red oak (Quercus rubra L.).

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