Total internal reflection microscopy (TIRM) as a nondestructive subsurface damage assessment tool

1995 ◽  
Author(s):  
Zhi M. Liao ◽  
Simon J. Cohen ◽  
John R. Taylor
Keyword(s):  
Damage Assessment ◽  
Total Internal Reflection ◽  
Assessment Tool ◽  
Subsurface Damage ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy

Study on Total Internal Reflection Microscopy for Subsurface Damage

2014 ◽  
Vol 34 (6) ◽  
pp. 0612004
Author(s):  
崔辉 Cui Hui ◽  
刘世杰 Liu Shijie ◽  
赵元安 Zhao Yuanan ◽  
杨俊 Yang Jun ◽  
刘杰 Liu Jie ◽  
...  
Keyword(s):  
Total Internal Reflection ◽  
Subsurface Damage ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy

scholarly journals Quantitative Evaluation of Subsurface Damage by Improved Total Internal Reflection Microscopy

2019 ◽  
Vol 9 (9) ◽  
pp. 1819 ◽  
Author(s):  
Kaizao Ni ◽  
Xin Cheng ◽  
Baoming Huang ◽  
Shijie Liu ◽  
Jianda Shao ◽  
...  
Keyword(s):  
Total Internal Reflection ◽  
Damage Threshold ◽  
Fused Silica ◽  
Subsurface Damage ◽  
Internal Reflection ◽  
Depth Of Field ◽  
Small Depth ◽  
Total Internal Reflection Microscopy ◽  
Laser Induced Damage ◽  
Definition Of

Subsurface damage (SSD), having a great impact on the laser-induced damage threshold (LIDT) of ultra-smooth optics applied in high-power laser systems, should be tightly controlled. An improved total internal reflection microscopy (TIRM), combined with digital image processing techniques, is proposed to quantitatively inspect SSD. With the characteristic that there is a relatively small depth of field (DOF) for a microscope at high magnification (50×), a series of SSD images are captured along with the microscope focusing at different depths under the surface by means of micro-focusing control. The definition of each image is calculated through wavelet transformation. By simulation, the relationship between the definition of TIRM images and the depth of the SSD has been established. According to the definition curve, the SSD depth is acquired. Fused silica glasses polished after fine grinding are measured non-destructively by our TIRM setup. The results show that the improved TIRM is a useful method to evaluate SSD. It is helpful to improve the efficiency of optical fabrication.

Measurement of Ca2+ signaling dynamics in exocrine cells with total internal reflection microscopy

2006 ◽  
Vol 291 (1) ◽  
pp. G146-G155 ◽  
Author(s):  
Jong Hak Won ◽  
David I. Yule
Keyword(s):  
Plasma Membrane ◽  
Total Internal Reflection ◽  
Acinar Cells ◽  
Internal Reflection ◽  
Pancreatic Acinar Cells ◽  
Wide Field ◽  
Exocrine Cells ◽  
Parotid Acinar Cells ◽  
Signaling Dynamics ◽  
Total Internal Reflection Microscopy

In nonexcitable cells, such as exocrine cells from the pancreas and salivary glands, agonist-stimulated Ca2+ signals consist of both Ca2+ release and Ca2+ influx. We have investigated the contribution of these processes to membrane-localized Ca2+ signals in pancreatic and parotid acinar cells using total internal reflection fluorescence (TIRF) microscopy (TIRFM). This technique allows imaging with unsurpassed resolution in a limited zone at the interface of the plasma membrane and the coverslip. In TIRFM mode, physiological agonist stimulation resulted in Ca2+ oscillations in both pancreas and parotid with qualitatively similar characteristics to those reported using conventional wide-field microscopy (WFM). Because local Ca2+ release in the TIRF zone would be expected to saturate the Ca2+ indicator (Fluo-4), these data suggest that Ca2+ release is occurring some distance from the area subjected to the measurement. When acini were stimulated with supermaximal concentrations of agonists, an initial peak, largely due to Ca2+ release, followed by a substantial, maintained plateau phase indicative of Ca2+ entry, was observed. The contribution of Ca2+ influx and Ca2+ release in isolation to these near-plasma membrane Ca2+ signals was investigated by using a Ca2+ readmission protocol. In the absence of extracellular Ca2+, the profile and magnitude of the initial Ca2+ release following stimulation with maximal concentrations of agonist or after SERCA pump inhibition were similar to those obtained with WFM in both pancreas and parotid acini. In contrast, when Ca2+ influx was isolated by subsequent Ca2+ readmission, the Ca2+ signals evoked were more robust than those measured with WFM. Furthermore, in parotid acinar cells, Ca2+ readdition often resulted in the apparent saturation of Fluo-4 but not of the low-affinity dye Fluo-4-FF. Interestingly, Ca2+ influx as measured by this protocol in parotid acinar cells was substantially greater than that initiated in pancreatic acinar cells. Indeed, robust Ca2+ influx was observed in parotid acinar cells even at low physiological concentrations of agonist. These data indicate that TIRFM is a useful tool to monitor agonist-stimulated near-membrane Ca2+ signals mediated by Ca2+ influx in exocrine acinar cells. In addition, TIRFM reveals that the extent of Ca2+ influx in parotid acinar cells is greater than pancreatic acinar cells when compared using identical methodologies.

scholarly journals A light scattering model for total internal reflection microscopy of geometrically anisotropic particles

2019 ◽  
Vol 66 (10) ◽  
pp. 1139-1151 ◽  
Author(s):  
Adrian Doicu ◽  
Alina A. Vasilyeva ◽  
Dmitry S. Efremenko ◽  
Christopher L. Wirth ◽  
Thomas Wriedt
Keyword(s):  
Light Scattering ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Anisotropic Particles ◽  
Scattering Model ◽  
Total Internal Reflection Microscopy
Sign in / Sign up

Export Citation Format

Share Document