The effect of laser irradiation on living cells incubated with gold nanoparticles

2015 ◽  
Author(s):  
Olga Bibikova ◽  
Prateek Singh ◽  
Alexey Popov ◽  
Georgy Akchurin ◽  
Ilya Skovorodkin ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Laser Irradiation ◽  
Living Cells

A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells

2016 ◽  
Vol 184 (2) ◽  
pp. 603-610 ◽  
Author(s):  
Man Li ◽  
Hongfang Gao ◽  
Xiaofei Wang ◽  
Yufeng Wang ◽  
Honglan Qi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticles ◽  
Tin Oxide ◽  
Living Cells ◽  
Oxide Electrode

Optoporation and Recovery of Living Cells under Au Nanoparticle Layer-Mediated NIR-Laser Irradiation

2021 ◽  
Author(s):  
Timofey E. Pylaev ◽  
Yuri Efremov ◽  
Elena S. Avdeeva ◽  
Artem A. Antoshin ◽  
Anastasiia I. Shpichka ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Living Cells ◽  
Au Nanoparticle ◽  
Nanoparticle Layer ◽  
Nir Laser

Rhodamine capped gold nanoparticles for the detection of Cr3+ ion in living cells and water samples

2018 ◽  
Vol 202 ◽  
pp. 282-288 ◽  
Author(s):  
N. Manjubaashini ◽  
T. Daniel Thangadurai ◽  
Ganapathi Bharathi ◽  
D. Nataraj
Keyword(s):  
Gold Nanoparticles ◽  
Water Samples ◽  
Living Cells

Multifunctional gold nanoparticles as smart nanovehicles with enhanced tumour-targeting abilities for intracellular pH mapping and in vivo MR/fluorescence imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 2002-2010 ◽  
Author(s):  
Kang-Kang Yu ◽  
Kun Li ◽  
Chun-Yan Lu ◽  
Yong-Mei Xie ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Living Cells ◽  
Tumour Targeting ◽  
Tumour Imaging ◽  
Pathological Changes

A number of multimodal agents have been developed for tumour imaging and diagnosis, but most of them cannot be used to study the detailed physiological or pathological changes in living cells at the same time.

Flower-shaped gold nanoparticles: synthesis, characterization and their application as SERS-active tags inside living cells

2010 ◽  
Vol 22 (5) ◽  
pp. 055702 ◽  
Author(s):  
Sanda Boca ◽  
Dumitrita Rugina ◽  
Adela Pintea ◽  
Lucian Barbu-Tudoran ◽  
Simion Astilean
Keyword(s):  
Gold Nanoparticles ◽  
Living Cells ◽  
Nanoparticles Synthesis

scholarly journals Effect of Laser Irradiation on Cell Function and Its Implications in Raman Spectroscopy

2018 ◽  
Vol 84 (8) ◽  
pp. e02508-17 ◽  
Author(s):  
Xiaofei Yuan ◽  
Yanqing Song ◽  
Yizhi Song ◽  
Jiabao Xu ◽  
Yinhu Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cell Growth ◽  
Single Cell ◽  
Laser Irradiation ◽  
Living Cells ◽  
Growth Characteristics ◽  
Single Cell Level ◽  
Bacterial Cells ◽  
Cell Level ◽  
Gram Negative

ABSTRACTLasers are instrumental in advanced bioimaging and Raman spectroscopy. However, they are also well known for their destructive effects on living organisms, leading to concerns about the adverse effects of laser technologies. To implement Raman spectroscopy for cell analysis and manipulation, such as Raman-activated cell sorting, it is crucial to identify nondestructive conditions for living cells. Here, we evaluated quantitatively the effect of 532-nm laser irradiation on bacterial cell fate and growth at the single-cell level. Using a purpose-built microfluidic platform, we were able to quantify the growth characteristics, i.e., specific growth rates and lag times of individual cells, as well as the survival rate of a population in conjunction with Raman spectroscopy. Representative Gram-negative and Gram-positive species show similar trends in response to a laser irradiation dose. Laser irradiation could compromise the physiological function of cells, and the degree of destruction is both dose and strain dependent, ranging from reduced cell growth to a complete loss of cell metabolic activity and finally to physical disintegration. Gram-positive bacterial cells are more susceptible than Gram-negative bacterial strains to irradiation-induced damage. By directly correlating Raman acquisition with single-cell growth characteristics, we provide evidence of nondestructive characteristics of Raman spectroscopy on individual bacterial cells. However, while strong Raman signals can be obtained without causing cell death, the variety of responses from different strains and from individual cells justifies careful evaluation of Raman acquisition conditions if cell viability is critical.IMPORTANCEIn Raman spectroscopy, the use of powerful monochromatic light in laser-based systems facilitates the detection of inherently weak signals. This allows environmentally and clinically relevant microorganisms to be measured at the single-cell level. The significance of being able to perform Raman measurement is that, unlike label-based fluorescence techniques, it provides a “fingerprint” that is specific to the identity and state of any (unlabeled) sample. Thus, it has emerged as a powerful method for studying living cells under physiological and environmental conditions. However, the laser's high power also has the potential to kill bacteria, which leads to concerns. The research presented here is a quantitative evaluation that provides a generic platform and methodology to evaluate the effects of laser irradiation on individual bacterial cells. Furthermore, it illustrates this by determining the conditions required to nondestructively measure the spectra of representative bacteria from several different groups.

scholarly journals Nucleation and growth of gold nanoparticles initiated by nanosecond and femtosecond laser irradiation of aqueous [AuCl4]−

2018 ◽  
Vol 20 (45) ◽  
pp. 28465-28475 ◽  
Author(s):  
Collin J. Rodrigues ◽  
Julian A. Bobb ◽  
Mallory G. John ◽  
Sergey P. Fisenko ◽  
M. Samy El-Shall ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Femtosecond Laser ◽  
Laser Irradiation ◽  
Surface Plasmon ◽  
Au Nanoparticles ◽  
Nucleation And Growth ◽  
Femtosecond Laser Irradiation ◽  
532 Nm ◽  
Polydisperse Particles ◽  
Autocatalytic Growth

Irradiation of aqueous [AuCl4]− with 532 nm, 8 ns pulses produces uniform 5 nm Au nanoparticles through surface plasmon-mediated photothermal autocatalytic growth, in contrast to the large polydisperse particles formed with 800 nm, 30 fs pulses.

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