Real-Time Temperature Monitoring of Photoinduced Cargo Release inside Living Cells Using Hybrid Capsules Decorated with Gold Nanoparticles and Fluorescent Nanodiamonds

2021 ◽  
Author(s):  
Elena N. Gerasimova ◽  
Vitaly V. Yaroshenko ◽  
Pavel M. Talianov ◽  
Oleksii O. Peltek ◽  
Mikhail A. Baranov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Real Time ◽  
Living Cells ◽  
Temperature Monitoring ◽  
Fluorescent Nanodiamonds

scholarly journals Temperature monitoring during light-induced release of cargo using polymer capsules modified with gold nanoparticles and nanodiamonds

2021 ◽  
Vol 2015 (1) ◽  
pp. 012045
Author(s):  
E N Gerasimova ◽  
V V Yaroshenko ◽  
P M Talianov ◽  
O O Peltek ◽  
L V Mikhailova ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Side Effects ◽  
Temperature Measurement ◽  
Real Time ◽  
Au Nanoparticles ◽  
Living Cells ◽  
Temperature Monitoring ◽  
Biological Objects ◽  
Multifunctional Platform

Abstract Application of different light-sensitive drug delivery carriers is limited due to a risk of overheating of living cells. Therefore, a real-time temperature monitoring within biological objects that controls the photothermal release of different cargos from light-sensitive carriers is highly demanded. In this work, we develop a multifunctional platform comprised of polymer microcapsules modified with nitrogen vacancies (NV) centers as nanothermometers and gold (Au) nanoparticles (NPs) as heating elements for the realization of laser-induced cargo release with a simultaneous temperature measurement inside cells. Such platform allows to prevent unwanted side effects related with the overheating of living cells and tissues.

Real-time tracking of the autophagy process in living cells using plasmonically enhanced Raman spectroscopy of fucoidan-coated gold nanoparticles

2018 ◽  
Vol 6 (34) ◽  
pp. 5460-5465 ◽  
Author(s):  
Hongje Jang ◽  
Kyungtae Kang ◽  
Mostafa A. El-Sayed
Keyword(s):  
Gold Nanoparticles ◽  
Raman Spectroscopy ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Molecular Level ◽  
Living Cells ◽  
Biological Event ◽  
Real Time Tracking

Fucoidan-coated gold nanoparticle mediated autophagy triggering and PERS monitoring provide molecular level insight on a biological event.

A multicolor DNA tetrahedron nanoprobe for analyzing human telomerase in living cells

2021 ◽  
Author(s):  
Ruiyuan Zhang ◽  
Ruixue Zhang ◽  
Wei Jiang ◽  
Xiaowen Xu
Keyword(s):  
Real Time ◽  
Length Distribution ◽  
Telomerase Activity ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Dna Tetrahedron ◽  
Human Telomerase

A sequentially lighting-up multicolor DNA tetrahedron nanoprobe is constructed for imaging telomerase activity, real-time monitoring telomerase action and determining product length distribution in living cells.

scholarly journals Design of a Real-Time Breakdown Voltage and On-Chip Temperature Monitoring System for Single Photon Avalanche Diodes

Electronics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 25
Author(s):  
Shijie Deng ◽  
Alan P. Morrison ◽  
Yong Guo ◽  
Chuanxin Teng ◽  
Ming Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Breakdown Voltage ◽  
Count Rate ◽  
Single Photon ◽  
Photon Counting ◽  
Temperature Monitoring ◽  
Dark Count ◽  
Chip Temperature ◽  
Avalanche Diodes ◽  
On Chip

The design and implementation of a real-time breakdown voltage and on-chip temperature monitoring system for single photon avalanche diodes (SPADs) is described in this work. In the system, an on-chip shaded (active area of the detector covered by a metal layer) SPAD is used to provide a dark count rate for the breakdown voltage and temperature calculation. A bias circuit was designed to provide a bias voltage scanning for the shaded SPAD. A microcontroller records the pulses from the anode of the shaded SPAD and calculates its real-time dark count rate. An algorithm was developed for the microcontroller to calculate the SPAD’s breakdown voltage and the on-chip temperature in real time. Experimental results show that the system is capable of measuring the SPAD’s breakdown voltage with a mismatch of less than 1.2%. Results also show that the system can provide real-time on-chip temperature monitoring for the range of −10 to 50 °C with errors of less than 1.7 °C. The system proposed can be used for the real-time SPAD’s breakdown voltage and temperature estimation for dual-SPADs or SPAD arrays chip where identical detectors are fabricated on the same chip and one or more dummy SPADs are shaded. With the breakdown voltage and the on-chip temperature monitoring, intelligent control logic can be developed to optimize the performance of the SPAD-based photon counting system by adjusting the parameters such as excess bias voltage and dead-time. This is particularly useful for SPAD photon counting systems used in complex working environments such as the applications in 3D LIDAR imaging for geodesy, geology, geomorphology, forestry, atmospheric physics and autonomous vehicles.

Sign in / Sign up

Export Citation Format

Share Document