Optoporation and Recovery of Living Cells under Au Nanoparticle Layer-Mediated NIR-Laser Irradiation

2021 ◽  
Author(s):  
Timofey E. Pylaev ◽  
Yuri Efremov ◽  
Elena S. Avdeeva ◽  
Artem A. Antoshin ◽  
Anastasiia I. Shpichka ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Living Cells ◽  
Au Nanoparticle ◽  
Nanoparticle Layer ◽  
Nir Laser

Ag functionalized SnS2 with enhanced photothermal activity for safe and efficient wound disinfection

2021 ◽  
Author(s):  
Jun Yang ◽  
Lin Sun ◽  
Shuhan Hui ◽  
Peng Zhang ◽  
Jian Li ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Antibacterial Effect ◽  
Photothermal Conversion ◽  
New Type ◽  
Nir Laser

A new type of Ag@SnS2 was established with Ag decorated on SnS2 surface for improved photothermal conversion capability. Ag@SnS2 exhibited a robust in vitro antibacterial effect and in vivo wound disinfection under NIR laser irradiation.

scholarly journals Plasmonic photothermal microneedle arrays and single needles for minimally-invasive deep in-skin hyperthermia

2020 ◽  
Vol 8 (25) ◽  
pp. 5425-5433 ◽  
Author(s):  
Álvaro Cárcamo-Martínez ◽  
Brónach Mallon ◽  
Juan Domínguez-Robles ◽  
A. Sara Cordeiro ◽  
Maurizio Celentano ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Laser Irradiation ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Skin Model ◽  
Thickness Skin ◽  
Microneedle Arrays ◽  
First Time ◽  
Nir Laser ◽  
Deep Hyperthermia

We report, for the first time, crosslinked polymeric microneedle (MN) arrays and single needles (2 mm and 4.5 mm length) coated with gold nanorods (GnRs) to induce deep hyperthermia in a 3 mm-thickness skin model upon near infrared (NIR) laser irradiation.

scholarly journals Effect of Laser Irradiation on Cell Function and Its Implications in Raman Spectroscopy

2018 ◽  
Vol 84 (8) ◽  
pp. e02508-17 ◽  
Author(s):  
Xiaofei Yuan ◽  
Yanqing Song ◽  
Yizhi Song ◽  
Jiabao Xu ◽  
Yinhu Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cell Growth ◽  
Single Cell ◽  
Laser Irradiation ◽  
Living Cells ◽  
Growth Characteristics ◽  
Single Cell Level ◽  
Bacterial Cells ◽  
Cell Level ◽  
Gram Negative

ABSTRACTLasers are instrumental in advanced bioimaging and Raman spectroscopy. However, they are also well known for their destructive effects on living organisms, leading to concerns about the adverse effects of laser technologies. To implement Raman spectroscopy for cell analysis and manipulation, such as Raman-activated cell sorting, it is crucial to identify nondestructive conditions for living cells. Here, we evaluated quantitatively the effect of 532-nm laser irradiation on bacterial cell fate and growth at the single-cell level. Using a purpose-built microfluidic platform, we were able to quantify the growth characteristics, i.e., specific growth rates and lag times of individual cells, as well as the survival rate of a population in conjunction with Raman spectroscopy. Representative Gram-negative and Gram-positive species show similar trends in response to a laser irradiation dose. Laser irradiation could compromise the physiological function of cells, and the degree of destruction is both dose and strain dependent, ranging from reduced cell growth to a complete loss of cell metabolic activity and finally to physical disintegration. Gram-positive bacterial cells are more susceptible than Gram-negative bacterial strains to irradiation-induced damage. By directly correlating Raman acquisition with single-cell growth characteristics, we provide evidence of nondestructive characteristics of Raman spectroscopy on individual bacterial cells. However, while strong Raman signals can be obtained without causing cell death, the variety of responses from different strains and from individual cells justifies careful evaluation of Raman acquisition conditions if cell viability is critical.IMPORTANCEIn Raman spectroscopy, the use of powerful monochromatic light in laser-based systems facilitates the detection of inherently weak signals. This allows environmentally and clinically relevant microorganisms to be measured at the single-cell level. The significance of being able to perform Raman measurement is that, unlike label-based fluorescence techniques, it provides a “fingerprint” that is specific to the identity and state of any (unlabeled) sample. Thus, it has emerged as a powerful method for studying living cells under physiological and environmental conditions. However, the laser's high power also has the potential to kill bacteria, which leads to concerns. The research presented here is a quantitative evaluation that provides a generic platform and methodology to evaluate the effects of laser irradiation on individual bacterial cells. Furthermore, it illustrates this by determining the conditions required to nondestructively measure the spectra of representative bacteria from several different groups.

Au nanoparticle-based probe for selenol in living cells and selenium-rich tea and rice

Talanta ◽  
2020 ◽  
Vol 212 ◽  
pp. 120583 ◽  
Author(s):  
Yadan Guo ◽  
Yi Luo ◽  
Ning Wang ◽  
Minggen Tang ◽  
Jingcheng Xiao ◽  
...  
Keyword(s):  
Living Cells ◽  
Au Nanoparticle

Performance Enhancement of All-Inorganic Perovskite Quantum Dots (CsPbX3) by UV-NIR Laser Irradiation

2019 ◽  
Vol 123 (7) ◽  
pp. 4502-4511 ◽  
Author(s):  
Ying Zhang ◽  
Haiou Zhu ◽  
Jilin Zheng ◽  
Guangyue Chai ◽  
Zongpeng Song ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Laser Irradiation ◽  
Performance Enhancement ◽  
Inorganic Perovskite ◽  
Perovskite Quantum Dots ◽  
Nir Laser

Concurrent photothermal therapy and photodynamic therapy for cutaneous squamous cell carcinoma by gold nanoclusters under a single NIR laser irradiation

2019 ◽  
Vol 7 (44) ◽  
pp. 6924-6933 ◽  
Author(s):  
Pei Liu ◽  
Weitao Yang ◽  
Lei Shi ◽  
Haiyan Zhang ◽  
Yan Xu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Cell Carcinoma ◽  
Laser Irradiation ◽  
Squamous Cell ◽  
Photothermal Therapy ◽  
Gold Nanoclusters ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Single Drug ◽  
Nir Laser

The concurrent photothermal and photodynamic therapy of cutaneous squamous cell carcinoma by a single drug of Au25(Capt)18 nanoclusters is demonstrated, together with a preliminary immune response study conducted under a single NIR laser irradiation.

scholarly journals A nuclear targeted dual-photosensitizer for drug-resistant cancer therapy with NIR activated multiple ROS

2016 ◽  
Vol 7 (7) ◽  
pp. 4237-4244 ◽  
Author(s):  
Zhengze Yu ◽  
Wei Pan ◽  
Na Li ◽  
Bo Tang
Keyword(s):  
Reactive Oxygen Species ◽  
Drug Resistance ◽  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Laser Irradiation ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Oxygen Species ◽  
Cancer Multiple ◽  
Nir Laser

A nuclear targeted dual-photosensitizer was developed for photodynamic therapy against multidrug resistant cancer. Multiple reactive oxygen species (ROS) could be generated in the nucleus to directly break DNA double strands with a single 980 nm NIR laser irradiation, regardless of drug resistance.

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