Label-free biochemical characterization of bovine sperm cells using Raman microscopy

2014 ◽  
Author(s):  
A. C. De Luca ◽  
S. Managò ◽  
M. A. Ferrara ◽  
L. Sirleto ◽  
R. Puglisi ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Raman Microscopy ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

Label-free biochemical characterization of bovine sperm cells using Raman microscopy

2013 ◽  
Author(s):  
A. C. De Luca ◽  
S. Manago ◽  
M. A. Ferrara ◽  
L. Sirleto ◽  
R. Puglisi ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Raman Microscopy ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

scholarly journals Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells

Biosensors ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 141-157 ◽  
Author(s):  
Maria Ferrara ◽  
Giuseppe Di Caprio ◽  
Stefano Managò ◽  
Annalisa De Angelis ◽  
Luigi Sirleto ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

scholarly journals Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells

2016 ◽  
Vol 34 (4) ◽  
pp. 1419-1423
Author(s):  
María L Chiapparrone ◽  
Pedro Soto ◽  
María Catena
Keyword(s):  
Sperm Cells ◽  
Bovine Sperm ◽  
Campylobacter Fetus ◽  
Fetus Subsp

Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach

2015 ◽  
Author(s):  
Annalisa De Angelis ◽  
Maria Antonietta Ferrara ◽  
Giuseppe Di Caprio ◽  
Stefano Manago ◽  
Luigi Sirleto ◽  
...  
Keyword(s):  
Raman Microscopy ◽  
Sperm Cells ◽  
Bovine Sperm

Label-free Imaging and Characterization of Cancer Cell Responses to Polymethoxyflavones Using Raman Microscopy

2016 ◽  
Vol 64 (51) ◽  
pp. 9708-9713 ◽  
Author(s):  
Hua Zhang ◽  
Jinkai Zheng ◽  
Anna Liu ◽  
Hang Xiao ◽  
Lili He
Keyword(s):  
Cancer Cell ◽  
Raman Microscopy ◽  
Label Free ◽  
Cell Responses

scholarly journals The swim-up technique separates bovine sperm by metabolic rates, motility and tail length

2019 ◽  
Author(s):  
Veronika Magdanz ◽  
Sergii Boryshpolets ◽  
Clara Ridzewski ◽  
Barbara Eckel ◽  
Klaus Reinhardt
Keyword(s):  
Sperm Quality ◽  
Tail Length ◽  
Sperm Cells ◽  
Metabolic Rates ◽  
Label Free ◽  
Purification Method ◽  
Bovine Sperm ◽  
Consumption Rates ◽  
Simple Step

AbstractSwim-up is a sperm purification method that is being used daily in andrology labs around the world as a simple step for in vitro sperm selection. This method accumulates the most motile sperm in the upper fraction and leaves sperm with low or no motility in the lower fraction but the underlying reasons are not fully understood. In this article, we compare metabolic rate, motility and sperm tail length of bovine sperm cells of the upper and lower fraction. The metabolic assay platform reveals oxygen consumption rates and extracellular acidification rates simultaneously and thereby delivers the metabolic rates in real time. Our study confirms the upper fraction of bull sperm has improved motility compared to the cells in the lower fraction and shows higher metabolic rates. This pattern was consistent across media of two different levels of viscosity. Sperm with longer flagella are selected in the upper fraction. We conclude that the motility-based separation of the swim-up technique is based on metabolic differences. Metabolic assays could serve as additional or alternative, label-free method to evaluate sperm quality, which is likely particularly useful in cases of asthenozoospermia and teratospermia. Furthermore, metabolic measurements of sperm cells can reveal differences in metabolic pathways in different environments.

scholarly journals Biochemical Profiles of In Vivo Oral Mucosa by Using a Portable Raman Spectroscopy System

Optics ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 134-147
Author(s):  
Marcelo Saito Nogueira ◽  
Victoria Ribeiro ◽  
Marianna Pires ◽  
Felipe Peralta ◽  
Luis Felipe das Chagas e Silva de Carvalho
Keyword(s):  
Raman Spectroscopy ◽  
Biochemical Characterization ◽  
Principal Component ◽  
Histopathological Analysis ◽  
Label Free ◽  
Healthy Individuals ◽  
Normal Tissues ◽  
The Difference

Most oral injuries are diagnosed by histopathological analysis of invasive and time-consuming biopsies. This analysis and conventional clinical observation cannot identify biochemically altered tissues predisposed to malignancy if no microstructural changes are detectable. With this in mind, detailed biochemical characterization of normal tissues and their differentiation features on healthy individuals is important in order to recognize biomolecular changes associated with early tissue predisposition to malignant transformation. Raman spectroscopy is a label-free method for characterization of tissue structure and specific composition. In this study, we used Raman spectroscopy to characterize the biochemistry of in vivo oral tissues of healthy individuals. We investigated this biochemistry based on the vibrational modes related to Raman spectra of four oral subsites (buccal, gingiva, lip and tongue) of ten volunteers as well as with principal component (PC) loadings for the difference between the four types of oral subsites. Therefore, we determined the biochemical characteristics of each type of healthy oral subsite and those corresponding to differentiation of the four types of subsites. In addition, we developed a spectral reference of oral healthy tissues of individuals in the Brazilian population for future diagnosis of early pathological conditions using real-time, noninvasive and label-free techniques such as Raman spectroscopy.

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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