Analysis of bovine sperm cells by a combined holographic and Raman microscopy approach

2015 ◽  
Author(s):  
Annalisa De Angelis ◽  
Maria Antonietta Ferrara ◽  
Giuseppe Di Caprio ◽  
Stefano Manago ◽  
Luigi Sirleto ◽  
...  
Keyword(s):  
Raman Microscopy ◽  
Sperm Cells ◽  
Bovine Sperm

Label-free biochemical characterization of bovine sperm cells using Raman microscopy

2013 ◽  
Author(s):  
A. C. De Luca ◽  
S. Manago ◽  
M. A. Ferrara ◽  
L. Sirleto ◽  
R. Puglisi ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Raman Microscopy ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

Label-free biochemical characterization of bovine sperm cells using Raman microscopy

2014 ◽  
Author(s):  
A. C. De Luca ◽  
S. Managò ◽  
M. A. Ferrara ◽  
L. Sirleto ◽  
R. Puglisi ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Raman Microscopy ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

scholarly journals Subcellular Localization of a Novel Alternative Splicing of IIIG9 and Colocalization with PPP1gamma Isoforms

2008 ◽  
Vol 14 (S3) ◽  
pp. 141-143
Author(s):  
C. Sousa ◽  
A.P. Vintém ◽  
M. Fardilha ◽  
O. da Cruz e Silva ◽  
E. da Cruz e Silva
Keyword(s):  
Alternative Splicing ◽  
Male Infertility ◽  
Cell Types ◽  
Sperm Cells ◽  
Regulatory Subunits ◽  
Specific Antibodies ◽  
Fluorescent Microscope ◽  
Bovine Sperm ◽  
Spermatogonia Cells ◽  
Different Cell Types

In testis we find mainly PPP1gamma2 isoform. We hypothesize that in different cell types we can find different regulatory subunits that may constitute targets for therapeutics of diseases such as male infertility, cancer and Alzheimer's disease. We identified a novel alternative splicing isoform of IIIG9 in testis, a known regulator of PPP1, IIIG9sT, and the aim of this study was its further characterization. We used a specific antibody for IIIG9sT in order to characterize its localization in bovine sperm cells. We also transfected IIIG9sT-GFP construct in mouse spermatogonia cells (GC-1 cells) and we used specific antibodies for each PPP1 isoform for the colocalization studies. We observed them under a fluorescent microscope and a LSM and quantified a high co-localization with PPP1gamma1 and 2 isoforms.

scholarly journals Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells

2016 ◽  
Vol 34 (4) ◽  
pp. 1419-1423
Author(s):  
María L Chiapparrone ◽  
Pedro Soto ◽  
María Catena
Keyword(s):  
Sperm Cells ◽  
Bovine Sperm ◽  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals Buserelin Acetate Reduces Mortality and DNA Defragmentation of Bovine Sperm Cells Exposed to Oxidative Stress

2020 ◽  
Vol 52 (5) ◽  
Author(s):  
Alicja Kowalczyk ◽  
Marian Kuczaj ◽  
Anna Szul ◽  
Ewa Czerniawska-Piatkowska
Keyword(s):  
Oxidative Stress ◽  
Sperm Cells ◽  
Bovine Sperm ◽  
Buserelin Acetate

scholarly journals Nanopolymer GluLa-DPG-PEG600-F Can Penetrate into Cells and Deposite in Rats Body

2019 ◽  
pp. 138-142
Author(s):  
Bogdan Chekh ◽  
Maria Ferens ◽  
Natalia Susol ◽  
Sergiy Varvarenko ◽  
Dmytro Ostapiv ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Survival ◽  
Metabolic Activity ◽  
Sperm Cell ◽  
Redox Activity ◽  
Sperm Cells ◽  
Oxygen Intake ◽  
Bovine Sperm ◽  
Muscle Tissues ◽  
High Doses

Via fluorescence microscopy we found that nanopolymer GluLa-DPG-PEG600-F can penetrate bovine sperm cells via binding membranes and deposit in the cite of injection in muscle tissues by binding proteins.Nanopolymer influences on sperm cells metabolic activity decreases oxygen intake and redox activity. We also determined sperm cell survival time, that was decreased of high doses of nanopolymer added to sperm.

Heat stress effects on bovine sperm cells: a chronological approach to early findings

2020 ◽  
Vol 64 (8) ◽  
pp. 1367-1378 ◽  
Author(s):  
Laura Nataly Garcia-Oliveros ◽  
Rubens Paes de Arruda ◽  
Leonardo Batissaco ◽  
Vitor Hugo Guilger Gonzaga ◽  
Vinícius José Moreira Nogueira ◽  
...  
Keyword(s):  
Heat Stress ◽  
Sperm Cells ◽  
Stress Effects ◽  
Bovine Sperm

Semen Sexing in Bovine: Current Status and the Need to Develop Alternative Techniques

2021 ◽  
Vol 1 (1) ◽  
pp. 17-31
Author(s):  
Himani Joshi ◽  
Manya Mathur ◽  
AK Mohanty ◽  
Sudarshan Kumar ◽  
JK Kaushik ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Review Paper ◽  
Current Status ◽  
Sperm Cells ◽  
Non Invasive ◽  
Bovine Sperm ◽  
The World ◽  
Alternative Techniques ◽  
Sperm Sorting ◽  
Cost Efficient

Livestock industry is one of the important pillars for country economy. Sperm sexing has the potential of significantly improving the quality and quantity of production in the livestock industries. Over years several attempts, involving several different parameters of sperm cells, have been made to develop an efficient sperm sorting procedure. Till date, the only commercially available method for bovine sperm is flow cytometry. The disadvantages associated with this approach, however, points towards the need for developing a more non-invasive and cost efficient approach for separation of X and Y chromosome bearing spermatozoa. In this review paper, we attempt to highlight all the previously developed approaches, as well as other emerging immunological and proteomics based methods that might have the potential of being developed as a promising breakthrough approach for semen sexing. Furthermore, we discuss the advantages and applications of semen sexing in India and around the world.

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