scholarly journals Label-free biochemical characterization of stem cells using vibrational spectroscopy

2009 ◽  
Vol 2 (11) ◽  
pp. 656-668 ◽  
Author(s):  
James W. Chan ◽  
Deborah K. Lieu
Keyword(s):  
Stem Cells ◽  
Vibrational Spectroscopy ◽  
Biochemical Characterization ◽  
Label Free

scholarly journals Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 750
Author(s):  
Pasquale Marrazzo ◽  
Valeria Pizzuti ◽  
Silvia Zia ◽  
Azzurra Sargenti ◽  
Daniele Gazzola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Defense Mechanisms ◽  
Live Cells ◽  
Label Free ◽  
Bacterial Diseases ◽  
Prospective Application ◽  
Therapy Strategies ◽  
Stromal Stem Cells ◽  
Different Sources

Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms.

scholarly journals Physical characterization of hematopoietic stem cells using multidirectional label-free light scatterings

2016 ◽  
Vol 24 (25) ◽  
pp. 28877 ◽  
Author(s):  
Hesam Shahin ◽  
Manisha Gupta ◽  
Anna Janowska-Wieczorek ◽  
Wojciech Rozmus ◽  
Ying Y. Tsui
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Physical Characterization ◽  
Label Free ◽  
Hematopoietic Stem

Label-free biochemical characterization of bovine sperm cells using Raman microscopy

2013 ◽  
Author(s):  
A. C. De Luca ◽  
S. Manago ◽  
M. A. Ferrara ◽  
L. Sirleto ◽  
R. Puglisi ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Raman Microscopy ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

scholarly journals Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells

Biosensors ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 141-157 ◽  
Author(s):  
Maria Ferrara ◽  
Giuseppe Di Caprio ◽  
Stefano Managò ◽  
Annalisa De Angelis ◽  
Luigi Sirleto ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

scholarly journals Role of Vibrational Spectroscopy in Stem Cell Research

2012 ◽  
Vol 27 ◽  
pp. 167-184 ◽  
Author(s):  
Ceren Aksoy ◽  
Feride Severcan
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Vibrational Spectroscopy ◽  
Cell Biology ◽  
Cell Types ◽  
Stem Cell Biology ◽  
Label Free ◽  
Monitoring Methods

Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the stem cells have some unwanted effects on the properties of stem cells, and these methods also do not provide real-time information about cellular conditions. These challenges enforce the usage of nondestructive, rapid, sensitive, high quality, label-free, cheep, and innovative chemical monitoring methods. In this context, vibrational spectroscopy provides promissing alternative to get new information into the field of stem cell biology for chemical analysis, quantification, and imaging of stem cells. Raman and infrared spectroscopy and imaging can be used as a new complimentary spectroscopic approaches to gain new insight into stem cell reseaches for future therapeutic and regenerative medicines. In this paper, recent developments in applications of vibrational spectroscopy techniques for stem cell characterization and identification are presented.

Label-free biochemical characterization of bovine sperm cells using Raman microscopy

2014 ◽  
Author(s):  
A. C. De Luca ◽  
S. Managò ◽  
M. A. Ferrara ◽  
L. Sirleto ◽  
R. Puglisi ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Raman Microscopy ◽  
Sperm Cells ◽  
Label Free ◽  
Bovine Sperm

Label-free identification and characterization of murine hair follicle stem cells located in thin tissue sections with Raman micro-spectroscopy

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2799-2805 ◽  
Author(s):  
Tsung-Hua Tsai ◽  
Michael A. Short ◽  
David I. McLean ◽  
Haishan Zeng ◽  
Kevin McElwee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Hair Follicle ◽  
Label Free ◽  
Tissue Sections ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells ◽  
Identification And Characterization

Stem cells offer tremendous opportunities for regenerative medicine.

scholarly journals Biochemical Profiles of In Vivo Oral Mucosa by Using a Portable Raman Spectroscopy System

Optics ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 134-147
Author(s):  
Marcelo Saito Nogueira ◽  
Victoria Ribeiro ◽  
Marianna Pires ◽  
Felipe Peralta ◽  
Luis Felipe das Chagas e Silva de Carvalho
Keyword(s):  
Raman Spectroscopy ◽  
Biochemical Characterization ◽  
Principal Component ◽  
Histopathological Analysis ◽  
Label Free ◽  
Healthy Individuals ◽  
Normal Tissues ◽  
The Difference

Most oral injuries are diagnosed by histopathological analysis of invasive and time-consuming biopsies. This analysis and conventional clinical observation cannot identify biochemically altered tissues predisposed to malignancy if no microstructural changes are detectable. With this in mind, detailed biochemical characterization of normal tissues and their differentiation features on healthy individuals is important in order to recognize biomolecular changes associated with early tissue predisposition to malignant transformation. Raman spectroscopy is a label-free method for characterization of tissue structure and specific composition. In this study, we used Raman spectroscopy to characterize the biochemistry of in vivo oral tissues of healthy individuals. We investigated this biochemistry based on the vibrational modes related to Raman spectra of four oral subsites (buccal, gingiva, lip and tongue) of ten volunteers as well as with principal component (PC) loadings for the difference between the four types of oral subsites. Therefore, we determined the biochemical characteristics of each type of healthy oral subsite and those corresponding to differentiation of the four types of subsites. In addition, we developed a spectral reference of oral healthy tissues of individuals in the Brazilian population for future diagnosis of early pathological conditions using real-time, noninvasive and label-free techniques such as Raman spectroscopy.

scholarly journals Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting

2017 ◽  
Vol 14 (133) ◽  
pp. 20170233 ◽  
Author(s):  
Miguel Xavier ◽  
María C. de Andrés ◽  
Daniel Spencer ◽  
Richard O. C. Oreffo ◽  
Hywel Morgan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dielectric Properties ◽  
Electrical Properties ◽  
Cell Size ◽  
Membrane Capacitance ◽  
Label Free ◽  
Biophysical Parameter ◽  
Mean Size

The capacity of bone and cartilage to regenerate can be attributed to skeletal stem cells (SSCs) that reside within the bone marrow (BM). Given SSCs are rare and lack specific surface markers, antibody-based sorting has failed to deliver the cell purity required for clinical translation. Microfluidics offers new methods of isolating cells based on biophysical features including, but not limited to, size, electrical properties and stiffness. Here we report the characterization of the dielectric properties of unexpanded SSCs using single-cell microfluidic impedance cytometry (MIC). Unexpanded SSCs had a mean size of 9.0 µm; larger than the majority of BM cells. During expansion, often used to purify and increase the number of SSCs, cell size and membrane capacitance increased significantly, highlighting the importance of characterizing unaltered SSCs. In addition, MIC was used to track the osteogenic differentiation of SSCs and showed an increased membrane capacitance with differentiation. The electrical properties of primary SSCs were indistinct from other BM cells precluding its use as an isolation method. However, the current studies indicate that cell size in combination with another biophysical parameter, such as stiffness, could be used to design label-free devices for sorting SSCs with significant clinical impact.

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