Thermal Stress Conditioning to Induce Osteogenic Protein Expression for Bone Regeneration

2012 ◽  
Author(s):  
Alana C. Sampson ◽  
Eunna Chung ◽  
Marissa Nichole Rylander
Keyword(s):  
Bone Regeneration ◽  
Bone Growth ◽  
Bone Defects ◽  
Regenerative Capacity ◽  
Wound Site ◽  
Essential Proteins ◽  
Osteogenic Protein ◽  
Exogenous Delivery ◽  
Critical Bone Defects ◽  
External Stimuli

Although bone has the intrinsic ability to “self-heal”, there are circumstances in which its regenerative capacity is limited or compromised, such as in critical bone defects. In these cases, the lack of osteogenic proteins at the wound site can prevent healing and external stimuli may be necessary to encourage bone growth [1]. Exogenous delivery of proteins and growth factors directly to the wound has been successful in bone regeneration, but is limited by the instability of the proteins and short half-lives. As a result, administration of multiple large doses of protein is necessary to retain a beneficial protein level. Due to these disadvantages, additional methods have been investigated to supply essential proteins to the bone defect [2].

3D-printed Poly-Lactic Co-Glycolic Acid (PLGA) scaffolds in non-critical bone defects impede bone regeneration in rabbit tibia bone

2021 ◽  
pp. 1-7
Author(s):  
Jin Xi Lim ◽  
Min He ◽  
Alphonsus Khin Sze Chong
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Volume Ratio ◽  
Bone Defects ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Rabbit Tibia ◽  
3D Printed ◽  
Critical Bone Defects

BACKGROUND: An increasing number of bone graft materials are commercially available and vary in their composition, mechanism of action, costs, and indications. OBJECTIVE: A commercially available PLGA scaffold produced using 3D printing technology has been used to promote the preservation of the alveolar socket after tooth extraction. We examined its influence on bone regeneration in long bones of New Zealand White rabbits. METHODS: 5.0-mm-diameter circular defects were created on the tibia bones of eight rabbits. Two groups were studied: (1) control group, in which the bone defects were left empty; (2) scaffold group, in which the PLGA scaffolds were implanted into the bone defect. Radiography was performed every two weeks postoperatively. After sacrifice, bone specimens were isolated and examined by micro-computed tomography and histology. RESULTS: Scaffolds were not degraded by eight weeks after surgery. Micro-computed tomography and histology showed that in the region of bone defects that was occupied by scaffolds, bone regeneration was compromised and the total bone volume/total volume ratio (BV/TV) was significantly lower. CONCLUSION: The implantation of this scaffold impedes bone regeneration in a non-critical bone defect. Implantation of bone scaffolds, if unnecessary, lead to a slower rate of fracture healing.

A Review of Platelet Derived Growth Factor Playing Pivotal Role in Bone Regeneration

2014 ◽  
Vol 40 (3) ◽  
pp. 330-340 ◽  
Author(s):  
Prasun Shah ◽  
Louise Keppler ◽  
James Rutkowski
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Bone Grafting ◽  
Bone Growth ◽  
Platelet Derived Growth Factor ◽  
Future Application ◽  
Second Phase ◽  
Bony Defect ◽  
Wound Site ◽  
Functioning Capillaries

This article is focused on the literature review and study of recent advances in the field of bone grafting, which involves platelet-derived growth factor (PDGF) as one of the facilitating factors in bone regeneration. This article includes a description of the mechanism of PDGF for use in surgeries where bone grafting is required, which promotes future application of PDGF for faster bone regeneration or inhibition of bone growth if required as in osteosarcoma. The important specific activities of PDGF include mitogenesis (increase in the cell populations of healing cells), angiogenesis (endothelial mitoses into functioning capillaries), and macrophage activation (debridement of the wound site and a second phase source of growth factors for continued repair and bone regeneration). Thus PDGF can be utilized in wound with bone defect to conceal the wound with repair of bony defect.

scholarly journals COMBINED USE OF HYALURONIC ACID WITH NANO-BIOACTIVE GLASS ENHANCED BIOCEMENT BASED SILICATE STIMULATED BONE REGENERATIVE CAPACITY IN TIBIAL BONE DEFECTS OF RABBITS: IN-VIVO STUDY

2021 ◽  
Vol 9 (5) ◽  
pp. 630-638
Author(s):  
Fatema Aziz Al-Sayed ◽  
◽  
Radwa Hamed Hegazy ◽  
Zeinab Amin Salem ◽  
Hanan Hassan El-Beheiry ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Bone Area ◽  
Regenerative Capacity ◽  
Tibial Bone ◽  
Group 3 ◽  
Area Percentage ◽  
Group 1

An ideal biomaterial for bone regeneration is a longstanding quest nowadays. This study aimed to evaluate the osteogenic potentiality of nano-bioactive glass enhanced biocement based silicate with or without hyaluronic acid seeded in rabbits’ tibial bone defects. For this, 24 male rabbits with two 5 mm defects (1 defect per tibia) were divided into three equal groups. Among the predefined three groups, for the rabbits of group 1(control) bone defects were left untreated while for the members of group 2 defects received nano-bioactive glass enhanced biocement based silicate cement, and group 3 defects received nano-bioactive glass cement mixed with hyaluronic acid. Animals of each group were divided equally for euthanization after 3 and 6 weeks. Bone specimens were processed and examined histologically with histomorphometrically analysis of new bone area percentage. The bone defects in group 3 showed significantly improved osseous healing histologically as compared to the group 1&2. The morphometric analysis also revealed a significant increase in the new bone area percentage in group 3 as compared to the group 1 and 2 (P < 0.05). The results of the present study can be concluded that bone defects could be treated with nano-bioactive glass and hyaluronic acid cement. Although, nano-bioactive glass alone was capable of bone regeneration the combination of both had significant regenerative capacity.

In vivo experimental study on bone regeneration in critical bone defects using an injectable biodegradable PLA/PGA copolymer

2005 ◽  
Vol 99 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Lia Rimondini ◽  
Nicolò Nicoli-Aldini ◽  
Milena Fini ◽  
Gaetano Guzzardella ◽  
Matilde Tschon ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Critical Bone Defects

scholarly journals Polydopamine functionalized VEGF gene-activated 3D printed scaffolds for bone regeneration

RSC Advances ◽  
2021 ◽  
Vol 11 (22) ◽  
pp. 13282-13291
Author(s):  
Jaidev L. Chakka ◽  
Timothy Acri ◽  
Noah Z. Laird ◽  
Ling Zhong ◽  
Kyungsup Shin ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Blood Vessels ◽  
Bone Defects ◽  
Vegf Gene ◽  
3D Printed ◽  
Critical Bone Defects

Bone is a highly vascularized organ and the formation of new blood vessels is essential to regenerate large critical bone defects.

scholarly journals Effects of collagen/β-tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects

2019 ◽  
Vol 17 (1) ◽  
pp. 228080001882049 ◽  
Author(s):  
Hamid Tebyanian ◽  
Mohammad Hadi Norahan ◽  
Hossein Eyni ◽  
Mansoureh Movahedin ◽  
SM Javad Mortazavi ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Bone Repair ◽  
Bone Growth ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Material Degradation ◽  
Alizarin Red ◽  
Calvarial Defects ◽  
Hematoxylin And Eosin

Bone defects remain a significant health issue and a major cause of morbidity in elderly patients. Composites based on collagen/calcium phosphate have been widely used for bone repair in clinical applications, owing to their comparability to bone extracellular matrix. This study aimed to evaluate the effects of a scaffold of collagen/calcium phosphate (COL/β-TCP) on bone formation to assess its potential use as a bone substitute to repair bone defects. Bilateral full-thickness critically sized calvarial defects (8 mm in diameter) were created in New Zealand white rabbits and treated with COL/β-TCP or COL scaffolds. One defect was also left unfilled as a control. Bone regeneration was assessed through histological evaluation using hematoxylin and eosin and Masson’s trichrome staining after 4 and 8 weeks. Alizarin Red staining was also utilized to observe the mineralization process. Our findings indicated that COL/β-TCP implantation could better enhance bone regeneration than COL and exhibited both new bone growth and scaffold material degradation.

Strontium folate loaded biohybrid scaffolds seeded with dental pulp stem cells induce in vivo bone regeneration in critical sized defects

2016 ◽  
Vol 4 (11) ◽  
pp. 1596-1604 ◽  
Author(s):  
Marcela Martin-del-Campo ◽  
Raul Rosales-Ibañez ◽  
Keila Alvarado ◽  
Jose G. Sampedro ◽  
Christian A. Garcia-Sepulveda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
Critical Bone Defects ◽  
Complete Regeneration

Strontium folate loaded biohybrid scaffolds enhance dental pulp stem cells replication and differentiation, promoting complete regeneration of critical bone defects.

scholarly journals In Vivo Investigation of Polymer-Ceramic PCL/HA and PCL/β-TCP 3D Composite Scaffolds and Electrical Stimulation for Bone Regeneration

Polymers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 65
Author(s):  
Júlia Venturini Helaehil ◽  
Carina Basqueira Lourenço ◽  
Boyang Huang ◽  
Luiza Venturini Helaehil ◽  
Isaque Xavier de Camargo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Electrical Stimulation ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
Electrical Therapy ◽  
Biological Recognition ◽  
3D Composite ◽  
Critical Bone Defects

Critical bone defects are a major clinical challenge in reconstructive bone surgery. Polycaprolactone (PCL) mixed with bioceramics, such as hydroxyapatite (HA) and tricalcium phosphate (TCP), create composite scaffolds with improved biological recognition and bioactivity. Electrical stimulation (ES) aims to compensate the compromised endogenous electrical signals and to stimulate cell proliferation and differentiation. We investigated the effects of composite scaffolds (PCL with HA; and PCL with β-TCP) and the use of ES on critical bone defects in Wistar rats using eight experimental groups: untreated, ES, PCL, PCL/ES, HA, HA/ES, TCP, and TCP/ES. The investigation was based on histomorphometry, immunohistochemistry, and gene expression analysis. The vascular area was greater in the HA/ES group on days 30 and 60. Tissue mineralization was greater in the HA, HA/ES, and TCP groups at day 30, and TCP/ES at day 60. Bmp-2 gene expression was higher in the HA, TCP, and TCP/ES groups at day 30, and in the TCP/ES and PCL/ES groups at day 60. Runx-2, Osterix, and Osteopontin gene expression were also higher in the TCP/ES group at day 60. These results suggest that scaffolds printed with PCL and TCP, when paired with electrical therapy application, improve bone regeneration.

Inductive biomaterials for bone regeneration

2017 ◽  
Vol 32 (6) ◽  
pp. 1047-1060 ◽  
Author(s):  
Rafid Kasir ◽  
Varadraj N. Vernekar ◽  
Cato T. Laurencin
Keyword(s):  
Bone Regeneration ◽  
Disease Transmission ◽  
Bone Growth ◽  
Bone Defects ◽  
The Other ◽  
Risk Of Disease ◽  
Autologous Bone ◽  
Materials Used ◽  
Autologous Bone Grafts ◽  
Induce Bone Formation

Inductive biomaterials are sought as alternatives to traditional materials used to treat bone defects. Traditional materials include autologous bone grafts that must be obtained surgically, and allografts that carry the risk of disease transmission and infection. Whereas the use of growth factors to stimulate bone growth has seen considerable advances, their efficacy is usually limited to supra-physiological doses with considerable side effects. On the other hand, certain biomaterials have an intrinsic ability to stimulate bone regeneration in lieu of growth factor use, and their use in repairing bone defects as well as improving the osteointegration of implants has been promising. These materials known as osteoinductive biomaterials include ceramics, metals, polymers, and composites of these materials. In this review, we examine the relevant properties of these different materials in their ability to induce bone formation.

scholarly journals Collagenous matrix as a predictor for bone formation: a digital technique for collagen quantification

2017 ◽  
Vol 22 (1) ◽  
Author(s):  
Gerson Arisoly Acasigua ◽  
Henrique Müller de Quevedo ◽  
Anna Christina Medeiros Fossati
Keyword(s):  
Bone Regeneration ◽  
Collagen Matrix ◽  
Bone Defects ◽  
Deciduous Teeth ◽  
Porous Scaffolds ◽  
Osteogenic Medium ◽  
Digital Technique ◽  
Collagenous Tissue ◽  
Collagenous Matrix ◽  
Critical Bone Defects

The collagenous matrix plays a fundamental role in the process of bone regeneration, so it is essential to study how it is primarily formed in situations in which critical bone defects are created. Objective: this study seeks to quantify the collagenous matrix formed in critical bone defects in the calvaria of mice over the process of bone regeneration promoted by the association of poly(lactide-co-glycolide) (PLGA) porous scaffolds and stem cells from deciduous teeth (SCDT). In addition, this study attempted to establish a precise protocol for the digital quantification of collagen through a histological method. Materials and method: Nine Wistar rats were used, in which critical defects of 8.0 mm of diameter were made in their calvarium. The animals were divided into three groups (n = 9): I – PLGA scaffolds; II – PLGA scaffolds/SCDT; III – PLGA scaffolds/SCDT maintained in osteogenic medium for 13 days. Within sixty postoperative days, calvaria were removed for histometric analysis following a digital protocol. A specific digital analysis method was designed for this study, in which a more precise quantification and differentiation between collagen fibers and non-collagenous tissue was possible, excluding factors that would normally alter the results. Results: it was noted that the association of PLGA scaffolds and SCDT maintained in osteogenic medium resulted in collagen matrix formation statistically higher than the other groups (p

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