Strontium folate loaded biohybrid scaffolds seeded with dental pulp stem cells induce in vivo bone regeneration in critical sized defects

2016 ◽  
Vol 4 (11) ◽  
pp. 1596-1604 ◽  
Author(s):  
Marcela Martin-del-Campo ◽  
Raul Rosales-Ibañez ◽  
Keila Alvarado ◽  
Jose G. Sampedro ◽  
Christian A. Garcia-Sepulveda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
Critical Bone Defects ◽  
Complete Regeneration

Strontium folate loaded biohybrid scaffolds enhance dental pulp stem cells replication and differentiation, promoting complete regeneration of critical bone defects.

scholarly journals Preconditioning with Melatonin Enhances Osteogenic Differentiation of Dental Pulp Mesenchymal Stem Cells by Regulating MAPK Pathways and Promotes the Efficiency of Bone Regeneration in Calvarial Bone Defects

2021 ◽  
Author(s):  
Ya-Hui Chan ◽  
Kuo-Ning Ho ◽  
Yu-Chieh Lee ◽  
Meng-Jung Chou ◽  
Wei-Zhen Lew ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Bone Defects ◽  
Calvarial Bone ◽  
Alp Activity ◽  
Cox 2

Abstract Background:Mesenchymal stem cell (MSC)-based tissue engineering plays a major role in regenerative medicine. However, the efficiency of MSC transplantation and survival of engrafted stem cells remain challenging. Melatonin can regulate MSC biology. However, its function in the osteogenic differentiation of dental pulp-derived MSCs (DPSCs) remains unclear. We investigated the effects and mechanisms of melatonin preconditioning on the osteogenic differentiation and bone regeneration capacities of DPSCs.Methods:The biological effects and signaling mechanisms of melatonin with different concentrations on DPSCs were evaluated using a proliferation assay, the quantitative alkaline phosphatase (ALP) activity, Alizarin red staining, a real-time polymerase chain reaction, and a western blot in vitro cell culture model. The in vivo bone regeneration capacities were assessed among empty control, MBCP, MBCP+DPSCs, and MBCP+DPSCs+melatonin preconditioning in four-created calvarial bone defects by using micro–computed tomographic, histological, histomorphometric, and immunofluorescence analyses after 4 and 8 weeks of healing.Results:In vitro experiments revealed that melatonin (1, 10, and 100 μM) significantly and concentration-dependently promoted proliferation, surface marker expression (CD 146), ALP activity and extracellular calcium deposition, and osteogenic gene expression of DPSCs (p < 0.05). Melatonin activated the phosphorylation of RUNX-2 and OCN and inhibited COX-2/NF-κB phosphorylation. Furthermore, the phosphorylation of mitogen-activated protein kinase (MAPK) P38/ERK signaling was significantly increased in DPSCs treated with 100 μM melatonin, and their inhibitors significantly decreased osteogenic differentiation. In vivo experiments demonstrated that bone defects implanted with MBCP bone-grafting materials and melatonin-preconditioned melatonin exhibited significantly greater bone volume fraction, trabecular bone structural modeling, new bone formation, and osteogenesis-related protein expression than the other three groups at 4 and 8 weeks postoperatively (p < 0.05).Conclusions:These results suggest that melatonin promotes the proliferation and osteogenic differentiation of DPSCs by regulating COX-2/NF-κB and p38/ERK MAPK signaling pathways. Preconditioning DPSCs with melatonin before transplantation can efficiently enhance MSC function and regenerative capacities.

Fibroin Scaffold Repairs Critical-Size Bone Defects In Vivo Supported by Human Amniotic Fluid and Dental Pulp Stem Cells

2012 ◽  
Vol 18 (9-10) ◽  
pp. 1006-1013 ◽  
Author(s):  
Massimo Riccio ◽  
Tullia Maraldi ◽  
Alessandra Pisciotta ◽  
Giovanni B. La Sala ◽  
Adriano Ferrari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Dental Pulp ◽  
Critical Size ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
Human Amniotic Fluid

P-EP013. Superior migration propensity of dental pulp stem cells in in vitro and in vivo models of excitotoxic neurodegeneration

2021 ◽  
Vol 132 (8) ◽  
pp. e82-e83
Author(s):  
Sivapriya Senthilkumar ◽  
Chaitra Venugopal ◽  
K. Shobha ◽  
Bindu M. Kutty ◽  
Anandh Dhanushkodi
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
In Vivo Models ◽  
Migration Propensity

scholarly journals The Interplay of Dental Pulp Stem Cells and Endothelial Cells in an Injectable Peptide Hydrogel on Angiogenesis and Pulp Regeneration In Vivo

2015 ◽  
Vol 21 (3-4) ◽  
pp. 550-563 ◽  
Author(s):  
Waruna Lakmal Dissanayaka ◽  
Kenneth M. Hargreaves ◽  
Lijian Jin ◽  
Lakshman P. Samaranayake ◽  
Chengfei Zhang
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Pulp Regeneration ◽  
Peptide Hydrogel

scholarly journals Dental pulp stem cells: Potential significance in regenerative medicine

2008 ◽  
Vol 55 (3) ◽  
pp. 170-179 ◽  
Author(s):  
Vera Todorovic ◽  
Dejan Markovic ◽  
Nadezda Milosevic-Jovcic ◽  
Marijana Petakov ◽  
Bela Balint ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Matrix Protein ◽  
Dental Pulp Stem Cells ◽  
Deciduous Teeth ◽  
High Plasticity ◽  
Dental Stem Cells ◽  
Periodontal Tissues ◽  
Extracellular Matrix Components

To date, three types of dental stem cells have been isolated: Dental Pulp Stem Cells (DPSC), Stem Cells From Human Exfoliated Deciduous Teeth (SHED) and Immature Dental Pulp Stem Cells (IDPC). These dental stem cells are considered as mesenchymal stem cells. They reside within the perivascular niche of dental pulp. They are highly proliferative, clonogenic, multipotent and are similar to mesenchymal Bone Marrow Stem Cells (BMSC). Also, they have high plasticity and can be easy isolated. The expressions of the alkaline phosphatase gene, dentin matrix protein 1 and dentinsialophosphoprotein are verified in these cells. Analyses of gene expression patterns indicated several genes which encode extracellular matrix components, cell adhesion molecules, growth factors and transcription regulators, cell signaling, cell communication or cell metabolism. In both conditions, in vivo and in vitro, these cells have the ability to differentiate into odontoblasts, chondrocytes, osteoblasts, adipocytes, neurons, melanocytes, smooth and skeletal muscles and endothelial cells. In vivo, after implantation, they have shown potential to differentiate into dentin but also into tissues like bone, adipose or neural tissue. In general, DPSCs are considered to have antiinflammatory and immunomodulatory abilities. After being grafted into allogenic tissues these cells are ableto induce immunological tolerance. Immunosuppressive effect is shown through the ability to inhibit proliferation of T lymphocytes. Dental pulp stem cells open new perspectives in therapeutic use not only in dentin regeneration, periodontal tissues and skeletoarticular, tissues of craniofacial region but also in treatment of neurotrauma, autoimmune diseases, myocardial infarction, muscular dystrophy and connective tissue damages.

scholarly journals Regenerative potential of human dental pulp stem cells in the treatment of stress urinary incontinence: In vitro and in vivo study

2019 ◽  
Vol 52 (6) ◽  
Author(s):  
Alessio Zordani ◽  
Alessandra Pisciotta ◽  
Laura Bertoni ◽  
Giulia Bertani ◽  
Antonio Vallarola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
In Vivo Study ◽  
Human Dental Pulp
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